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Q67QZ1 (NAPA_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:STH917
OrganismSymbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]
Taxonomic identifier292459 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Secreted Potential. Note: Membrane-associated Potential. HAMAP-Rule MF_01630

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Ontologies

Keywords
   Biological processElectron transport
Nitrate assimilation
Transport
   Cellular componentSecreted
   DomainSignal
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
Molybdenum
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processnitrate assimilation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

molybdenum ion binding

Inferred from electronic annotation. Source: InterPro

nitrate reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 754723Nitrate reductase HAMAP-Rule MF_01630
PRO_0000046008

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q67QZ1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 2754BFA565A43AF0

FASTA75483,580
        10         20         30         40         50         60 
MKFTRRSFVK ASALATAMVA AGCSPQPVAP KQNPETEGAT WYKTVCRYCG VGCGVMVAAK 

        70         80         90        100        110        120 
DNRVVAVKGD TENPVNKGLL CVKGYYLDRI MNTEEGRILK PLIRKNGQLT EASWDEALDL 

       130        140        150        160        170        180 
VAARFREAID QHGPDSVGFY GSGQNLAEET YIANKLFKGC IGTNNVEGNP RTCMASAVAG 

       190        200        210        220        230        240 
FLSTFGKDEP MGNLDDIEHA DTFFIIGSNT AEAHPIIYSR ITTRKQTGKD VKVILADPRR 

       250        260        270        280        290        300 
HRVADIADIF LPFRPGTDLA LLNAFAQVII EEGLHDPDFI ERHTTFQDGN GAITFEQYVA 

       310        320        330        340        350        360 
FLQDYTPEKV APITGLSPDD IRAAAREIGA RGRKTMTLWC MGINQRTVGT WLNNAIYNLH 

       370        380        390        400        410        420 
LLTGKICQPG NSPFSLTGQP SACGSVREGG GLSHLLPCGR QVTNEQHRKE VAAVWGVDYT 

       430        440        450        460        470        480 
RMSDKVGYHT MEMFRAAGDG RIKALLISCT NPGHSLPNLN SVRASLEKTF LVVMDAFHNR 

       490        500        510        520        530        540 
TTELADVVLP SALWCEKEGI YGNTERRTQH LAKAVEPKGE ARPDVWILLE IAKRLGYGEY 

       550        560        570        580        590        600 
FSHYTSNEVI WEEFRKMGGG GTGYDYAPYE RYKQERGLRW PVNDKQPAGT TLRYVEGDDP 

       610        620        630        640        650        660 
FVPEGAGIYF YGKPDGKAVI YARPHRDPAE VPDAEYPFYL STGRILEHWH TITMTKRVPE 

       670        680        690        700        710        720 
IMKGAGEFYC EIHEEDAARL GIQNGDLVKL TSRRGQVVAT ARVGGRAVPQ KGLVFLLMHD 

       730        740        750 
DRTERLANFL TNDAVDETSK QMEYKVCAVR VEKA 

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References

[1]"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T / IAM 14863.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006840 Genomic DNA. Translation: BAD39902.1.
RefSeqYP_074746.1. NC_006177.1.

3D structure databases

ProteinModelPortalQ67QZ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292459.STH917.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD39902; BAD39902; STH917.
GeneID2979700.
KEGGsth:STH917.
PATRIC23779962. VBISymThe116959_0911.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFHAVLQD.
OrthoDBEOG6CVV7G.
ProtClustDBCLSK748640.

Enzyme and pathway databases

BioCycSTHE292459:GJMM-977-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SYMTH
AccessionPrimary (citable) accession number: Q67QZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families