Reviewed, UniProtKB/Swiss-Prot Q67QZ1 (NAPA_SYMTH)

Names and origin

Protein names

Recommended name:
    Nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	STH917

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Secreted Potential. Note: Membrane-associated Potential.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Secreted
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 754	723	Nitrate reductase HAMAP MF_01630		PRO_0000046008
Sites
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67QZ1-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2754BFA565A43AF0	FASTA	754	83,580
	10 20 30 40 50 60 MKFTRRSFVK ASALATAMVA AGCSPQPVAP KQNPETEGAT WYKTVCRYCG VGCGVMVAAK 70 80 90 100 110 120 DNRVVAVKGD TENPVNKGLL CVKGYYLDRI MNTEEGRILK PLIRKNGQLT EASWDEALDL 130 140 150 160 170 180 VAARFREAID QHGPDSVGFY GSGQNLAEET YIANKLFKGC IGTNNVEGNP RTCMASAVAG 190 200 210 220 230 240 FLSTFGKDEP MGNLDDIEHA DTFFIIGSNT AEAHPIIYSR ITTRKQTGKD VKVILADPRR 250 260 270 280 290 300 HRVADIADIF LPFRPGTDLA LLNAFAQVII EEGLHDPDFI ERHTTFQDGN GAITFEQYVA 310 320 330 340 350 360 FLQDYTPEKV APITGLSPDD IRAAAREIGA RGRKTMTLWC MGINQRTVGT WLNNAIYNLH 370 380 390 400 410 420 LLTGKICQPG NSPFSLTGQP SACGSVREGG GLSHLLPCGR QVTNEQHRKE VAAVWGVDYT 430 440 450 460 470 480 RMSDKVGYHT MEMFRAAGDG RIKALLISCT NPGHSLPNLN SVRASLEKTF LVVMDAFHNR 490 500 510 520 530 540 TTELADVVLP SALWCEKEGI YGNTERRTQH LAKAVEPKGE ARPDVWILLE IAKRLGYGEY 550 560 570 580 590 600 FSHYTSNEVI WEEFRKMGGG GTGYDYAPYE RYKQERGLRW PVNDKQPAGT TLRYVEGDDP 610 620 630 640 650 660 FVPEGAGIYF YGKPDGKAVI YARPHRDPAE VPDAEYPFYL STGRILEHWH TITMTKRVPE 670 680 690 700 710 720 IMKGAGEFYC EIHEEDAARL GIQNGDLVKL TSRRGQVVAT ARVGGRAVPQ KGLVFLLMHD 730 740 750 DRTERLANFL TNDAVDETSK QMEYKVCAVR VEKA

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006840 Genomic DNA. Translation: BAD39902.1.
RefSeq	YP_074746.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2979700.
GenomeReviews	Gene locus STH917 in contig AP006840_GR.
KEGG	sth:STH917.
NMPDR	fig|292459.1.peg.881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q67QZ1.
OMA	ERRTQAW.
Enzyme and pathway databases
BioCyc	STHE292459:STH917-MON.
BRENDA	1.7.99.4. 20615.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. TAT_signal. IPR019546. TAT_signal_subgr. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_SYMTH

Accession

Primary (citable) accession number: Q67QZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)