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Q67QZ1

- NAPA_SYMTH

UniProt

Q67QZ1 - NAPA_SYMTH

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Protein

Nitrate reductase

Gene

napA

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. molybdenum ion binding Source: InterPro
  3. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-977-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:STH917
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium
ProteomesiUP000000417: Chromosome

Subcellular locationi

Secreted UniRule annotation
Note: Membrane-associated.UniRule annotation

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 754723Nitrate reductasePRO_0000046008Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi292459.STH917.

Structurei

3D structure databases

ProteinModelPortaliQ67QZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q67QZ1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKFTRRSFVK ASALATAMVA AGCSPQPVAP KQNPETEGAT WYKTVCRYCG
60 70 80 90 100
VGCGVMVAAK DNRVVAVKGD TENPVNKGLL CVKGYYLDRI MNTEEGRILK
110 120 130 140 150
PLIRKNGQLT EASWDEALDL VAARFREAID QHGPDSVGFY GSGQNLAEET
160 170 180 190 200
YIANKLFKGC IGTNNVEGNP RTCMASAVAG FLSTFGKDEP MGNLDDIEHA
210 220 230 240 250
DTFFIIGSNT AEAHPIIYSR ITTRKQTGKD VKVILADPRR HRVADIADIF
260 270 280 290 300
LPFRPGTDLA LLNAFAQVII EEGLHDPDFI ERHTTFQDGN GAITFEQYVA
310 320 330 340 350
FLQDYTPEKV APITGLSPDD IRAAAREIGA RGRKTMTLWC MGINQRTVGT
360 370 380 390 400
WLNNAIYNLH LLTGKICQPG NSPFSLTGQP SACGSVREGG GLSHLLPCGR
410 420 430 440 450
QVTNEQHRKE VAAVWGVDYT RMSDKVGYHT MEMFRAAGDG RIKALLISCT
460 470 480 490 500
NPGHSLPNLN SVRASLEKTF LVVMDAFHNR TTELADVVLP SALWCEKEGI
510 520 530 540 550
YGNTERRTQH LAKAVEPKGE ARPDVWILLE IAKRLGYGEY FSHYTSNEVI
560 570 580 590 600
WEEFRKMGGG GTGYDYAPYE RYKQERGLRW PVNDKQPAGT TLRYVEGDDP
610 620 630 640 650
FVPEGAGIYF YGKPDGKAVI YARPHRDPAE VPDAEYPFYL STGRILEHWH
660 670 680 690 700
TITMTKRVPE IMKGAGEFYC EIHEEDAARL GIQNGDLVKL TSRRGQVVAT
710 720 730 740 750
ARVGGRAVPQ KGLVFLLMHD DRTERLANFL TNDAVDETSK QMEYKVCAVR

VEKA
Length:754
Mass (Da):83,580
Last modified:October 11, 2004 - v1
Checksum:i2754BFA565A43AF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39902.1.
RefSeqiYP_074746.1. NC_006177.1.

Genome annotation databases

EnsemblBacteriaiBAD39902; BAD39902; STH917.
GeneIDi2979700.
KEGGisth:STH917.
PATRICi23779962. VBISymThe116959_0911.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39902.1 .
RefSeqi YP_074746.1. NC_006177.1.

3D structure databases

ProteinModelPortali Q67QZ1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 292459.STH917.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD39902 ; BAD39902 ; STH917 .
GeneIDi 2979700.
KEGGi sth:STH917.
PATRICi 23779962. VBISymThe116959_0911.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci STHE292459:GJMM-977-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
    Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
    Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T / IAM 14863.

Entry informationi

Entry nameiNAPA_SYMTH
AccessioniPrimary (citable) accession number: Q67QZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 11, 2004
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3