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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi258 – 2581MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-1166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheSUniRule annotation
Synonyms:pheSA
Ordered Locus Names:STH1105
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSymbiobacteriaceaeSymbiobacterium
ProteomesiUP000000417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Phenylalanine--tRNA ligase alpha subunitPRO_0000126779Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi292459.STH1105.

Structurei

3D structure databases

ProteinModelPortaliQ67QF3.
SMRiQ67QF3. Positions 83-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0016.
HOGENOMiHOG000242675.
KOiK01889.
OMAiKGTGWLE.
OrthoDBiEOG6WX4QN.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q67QF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDQLLKLRD EALAAIAAAA DVGAIQEMRV RYLGKKSELS QVLGGLGRLP
60 70 80 90 100
TVEERKAMGA LGNEIKQAIT AALDAREAEL AAAALQARLA SERIDVTLPG
110 120 130 140 150
APVRRGHLHI LNQVIHRIEE IFIAMGYEVA ESRQVETDWY NFEALNIPKG
160 170 180 190 200
HPARDAQDSL FLSEEVLLRT HTSNTQIRYM LEVARGRTPV KIICPGRVFR
210 220 230 240 250
RDFEDATHAM MFHQVEGLVI DKGITMASLK GALTEMARAL FGPDVGIRLR
260 270 280 290 300
PSYFPFTEPS AEMDISCIFC GGKGCRTCKG SGWIEIGGSG MVHPNVLRAG
310 320 330 340
GYDPEEVSGW AFGYGPERVA MLMYGIEDIR HFVNNDMRFL RQF
Length:343
Mass (Da):37,988
Last modified:October 11, 2004 - v1
Checksum:i920E073E177DCE5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD40090.1.
RefSeqiWP_011195237.1. NC_006177.1.

Genome annotation databases

EnsemblBacteriaiBAD40090; BAD40090; STH1105.
KEGGisth:STH1105.
PATRICi23780354. VBISymThe116959_1106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD40090.1.
RefSeqiWP_011195237.1. NC_006177.1.

3D structure databases

ProteinModelPortaliQ67QF3.
SMRiQ67QF3. Positions 83-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292459.STH1105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD40090; BAD40090; STH1105.
KEGGisth:STH1105.
PATRICi23780354. VBISymThe116959_1106.

Phylogenomic databases

eggNOGiCOG0016.
HOGENOMiHOG000242675.
KOiK01889.
OMAiKGTGWLE.
OrthoDBiEOG6WX4QN.

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-1166-MONOMER.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
    Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
    Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T / IAM 14863.

Entry informationi

Entry nameiSYFA_SYMTH
AccessioniPrimary (citable) accession number: Q67QF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 11, 2004
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.