Q67QF3 (SYFA_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Synonyms:	pheSA
Ordered Locus Names:	STH1105

Organism

Symbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]

Taxonomic identifier

292459 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales incertae sedis › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium › Symbiobacterium thermophilum

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00281
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_0000126779
Sites
	Metal binding	258	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67QF3 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 920E073E177DCE5E	FASTA	343	37,988
	10 20 30 40 50 60 MKDQLLKLRD EALAAIAAAA DVGAIQEMRV RYLGKKSELS QVLGGLGRLP TVEERKAMGA 70 80 90 100 110 120 LGNEIKQAIT AALDAREAEL AAAALQARLA SERIDVTLPG APVRRGHLHI LNQVIHRIEE 130 140 150 160 170 180 IFIAMGYEVA ESRQVETDWY NFEALNIPKG HPARDAQDSL FLSEEVLLRT HTSNTQIRYM 190 200 210 220 230 240 LEVARGRTPV KIICPGRVFR RDFEDATHAM MFHQVEGLVI DKGITMASLK GALTEMARAL 250 260 270 280 290 300 FGPDVGIRLR PSYFPFTEPS AEMDISCIFC GGKGCRTCKG SGWIEIGGSG MVHPNVLRAG 310 320 330 340 GYDPEEVSGW AFGYGPERVA MLMYGIEDIR HFVNNDMRFL RQF

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD40090.1.
RefSeq	YP_074934.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q67QF3.
SMR	Q67QF3. Positions 83-343.
ModBase	Search...
Protein-protein interaction databases
STRING	292459.STH1105.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD40090; BAD40090; STH1105.
GeneID	2979552.
KEGG	sth:STH1105.
PATRIC	23780354. VBISymThe116959_1106.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HOG000242675.
KO	K01889.
OMA	LTHTPMF.
Enzyme and pathway databases
BioCyc	STHE292459:GJMM-1166-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_SYMTH

Accession

Primary (citable) accession number: Q67QF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program