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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei559Aminoacyl-adenylateUniRule annotation1
Binding sitei604ATPUniRule annotation1
Metal bindingi904ZincUniRule annotation1
Metal bindingi907ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSTHE292459:G1G28-1263-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:STH1231
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSymbiobacteriaceaeSymbiobacterium
Proteomesi
  • UP000000417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984881 – 938Isoleucine--tRNA ligaseAdd BLAST938

Proteomic databases

PRIDEiQ67Q27

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi292459.STH1231

Structurei

3D structure databases

ProteinModelPortaliQ67Q27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 71"HIGH" regionAdd BLAST11
Motifi601 – 605"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q67Q27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKTDYKAT LNMPRTDFPM RANLPTREPE QLKKWEEMDL YNLVQRATAG
60 70 80 90 100
RPKFVLHDGP PYANGDIHLG TALNKILKDI IVKHATMAGY DAPYVPGWDM
110 120 130 140 150
HGLPIELRAL KDMNIDRRKI DPLELRAKCY EYAHHWLNVQ REQFKRLGVR
160 170 180 190 200
GDWENPYRTV APEFEAKEVE VFGAMAAKGY IYRGLKPVYW CPYCETALAE
210 220 230 240 250
AEIEYNEKTS YSIYVRFPVV DPRGKLPEGS YLVIWTTTPW TIPANLAVAV
260 270 280 290 300
HPEVEYGVYA TEKGNLVVAT ALAEKFFQAV NLPAAEPVAT LKGADLEGIT
310 320 330 340 350
YRHLLYDRVS PVILGDHVTT EDGTGLVHTA PGHGHEDFEV GQKYGLPVLN
360 370 380 390 400
PVNDQGVFTA EAGPFAGMFI EKANPEIIKA LDEAGMLLGQ GKIRHQYAHC
410 420 430 440 450
WRCKNPVIYR ATVQWFVKVE GFMDIAKEAM NHVRWIPDWG YNRMYAMIDG
460 470 480 490 500
LADWCISRQR AWGLPIPILT CSACDEPSFE PKMFEKIAEI FRAEGSDAWW
510 520 530 540 550
RRPAEDFMPE GGLTCKKCGG RTFHKEKDIL DVWFDSGSSH VGVLETRPEL
560 570 580 590 600
TWPADLYLEG SDQHRGWFKS SLLTAVVARD GKPPYKAVLT HGFTVDEQGR
610 620 630 640 650
KMSKSLGNVV DPADVIKRYG ADILRLWVAS TDYRHDMALS ENILKQVADA
660 670 680 690 700
YRKIRNTLRY LLGNLYDFNP DTDMVERDDL LEIDRWQMHR LQEVIRKVTE
710 720 730 740 750
AYREYEYHIV YHTLNNYCAV DLSAVYLDIL KDRLYTSAPA SRERRSAQTV
760 770 780 790 800
LYHVADALIR MLTPILTFTA EEAYSHLPKP AGSPPTSQLL MMPQPDPAYL
810 820 830 840 850
DENLAAEWDR LMELRDAVQV VLERARVDKL IGSSQEAAVN LYASGGEGSW
860 870 880 890 900
AELLDRHLPD LPSIFIVSDV KLFVGGQSAP SGTYFGEGPG DLSVEVVRAE
910 920 930
GEKCERCWNY RKVGAIEQHP TLCERCAGVV LSLNLDNA
Length:938
Mass (Da):106,189
Last modified:October 11, 2004 - v1
Checksum:iA21A5E420CA8C381
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA Translation: BAD40216.1
RefSeqiWP_011195362.1, NC_006177.1

Genome annotation databases

EnsemblBacteriaiBAD40216; BAD40216; STH1231
KEGGisth:STH1231

Similar proteinsi

Entry informationi

Entry nameiSYI_SYMTH
AccessioniPrimary (citable) accession number: Q67Q27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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