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Q67Q27

- SYI_SYMTH

UniProt

Q67Q27 - SYI_SYMTH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei604 – 6041ATPUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSTHE292459:GJMM-1300-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:STH1231
    OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
    Taxonomic identifieri292459 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium
    ProteomesiUP000000417: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098488Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi292459.STH1231.

    Structurei

    3D structure databases

    ProteinModelPortaliQ67Q27.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi601 – 6055"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q67Q27-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEKTDYKAT LNMPRTDFPM RANLPTREPE QLKKWEEMDL YNLVQRATAG    50
    RPKFVLHDGP PYANGDIHLG TALNKILKDI IVKHATMAGY DAPYVPGWDM 100
    HGLPIELRAL KDMNIDRRKI DPLELRAKCY EYAHHWLNVQ REQFKRLGVR 150
    GDWENPYRTV APEFEAKEVE VFGAMAAKGY IYRGLKPVYW CPYCETALAE 200
    AEIEYNEKTS YSIYVRFPVV DPRGKLPEGS YLVIWTTTPW TIPANLAVAV 250
    HPEVEYGVYA TEKGNLVVAT ALAEKFFQAV NLPAAEPVAT LKGADLEGIT 300
    YRHLLYDRVS PVILGDHVTT EDGTGLVHTA PGHGHEDFEV GQKYGLPVLN 350
    PVNDQGVFTA EAGPFAGMFI EKANPEIIKA LDEAGMLLGQ GKIRHQYAHC 400
    WRCKNPVIYR ATVQWFVKVE GFMDIAKEAM NHVRWIPDWG YNRMYAMIDG 450
    LADWCISRQR AWGLPIPILT CSACDEPSFE PKMFEKIAEI FRAEGSDAWW 500
    RRPAEDFMPE GGLTCKKCGG RTFHKEKDIL DVWFDSGSSH VGVLETRPEL 550
    TWPADLYLEG SDQHRGWFKS SLLTAVVARD GKPPYKAVLT HGFTVDEQGR 600
    KMSKSLGNVV DPADVIKRYG ADILRLWVAS TDYRHDMALS ENILKQVADA 650
    YRKIRNTLRY LLGNLYDFNP DTDMVERDDL LEIDRWQMHR LQEVIRKVTE 700
    AYREYEYHIV YHTLNNYCAV DLSAVYLDIL KDRLYTSAPA SRERRSAQTV 750
    LYHVADALIR MLTPILTFTA EEAYSHLPKP AGSPPTSQLL MMPQPDPAYL 800
    DENLAAEWDR LMELRDAVQV VLERARVDKL IGSSQEAAVN LYASGGEGSW 850
    AELLDRHLPD LPSIFIVSDV KLFVGGQSAP SGTYFGEGPG DLSVEVVRAE 900
    GEKCERCWNY RKVGAIEQHP TLCERCAGVV LSLNLDNA 938
    Length:938
    Mass (Da):106,189
    Last modified:October 11, 2004 - v1
    Checksum:iA21A5E420CA8C381
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006840 Genomic DNA. Translation: BAD40216.1.
    RefSeqiYP_075060.1. NC_006177.1.

    Genome annotation databases

    EnsemblBacteriaiBAD40216; BAD40216; STH1231.
    GeneIDi2979836.
    KEGGisth:STH1231.
    PATRICi23780624. VBISymThe116959_1233.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006840 Genomic DNA. Translation: BAD40216.1 .
    RefSeqi YP_075060.1. NC_006177.1.

    3D structure databases

    ProteinModelPortali Q67Q27.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 292459.STH1231.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD40216 ; BAD40216 ; STH1231 .
    GeneIDi 2979836.
    KEGGi sth:STH1231.
    PATRICi 23780624. VBISymThe116959_1233.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci STHE292459:GJMM-1300-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
      Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
      Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: T / IAM 14863.

    Entry informationi

    Entry nameiSYI_SYMTH
    AccessioniPrimary (citable) accession number: Q67Q27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3