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Q67Q27

- SYI_SYMTH

UniProt

Q67Q27 - SYI_SYMTH

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Protein
Isoleucine--tRNA ligase
Gene
ileS, STH1231
Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei604 – 6041ATP By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-1300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:STH1231
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium
ProteomesiUP000000417: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098488Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi292459.STH1231.

Structurei

3D structure databases

ProteinModelPortaliQ67Q27.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionUniRule annotation
Add
BLAST
Motifi601 – 6055"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q67Q27-1 [UniParc]FASTAAdd to Basket

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MAEKTDYKAT LNMPRTDFPM RANLPTREPE QLKKWEEMDL YNLVQRATAG    50
RPKFVLHDGP PYANGDIHLG TALNKILKDI IVKHATMAGY DAPYVPGWDM 100
HGLPIELRAL KDMNIDRRKI DPLELRAKCY EYAHHWLNVQ REQFKRLGVR 150
GDWENPYRTV APEFEAKEVE VFGAMAAKGY IYRGLKPVYW CPYCETALAE 200
AEIEYNEKTS YSIYVRFPVV DPRGKLPEGS YLVIWTTTPW TIPANLAVAV 250
HPEVEYGVYA TEKGNLVVAT ALAEKFFQAV NLPAAEPVAT LKGADLEGIT 300
YRHLLYDRVS PVILGDHVTT EDGTGLVHTA PGHGHEDFEV GQKYGLPVLN 350
PVNDQGVFTA EAGPFAGMFI EKANPEIIKA LDEAGMLLGQ GKIRHQYAHC 400
WRCKNPVIYR ATVQWFVKVE GFMDIAKEAM NHVRWIPDWG YNRMYAMIDG 450
LADWCISRQR AWGLPIPILT CSACDEPSFE PKMFEKIAEI FRAEGSDAWW 500
RRPAEDFMPE GGLTCKKCGG RTFHKEKDIL DVWFDSGSSH VGVLETRPEL 550
TWPADLYLEG SDQHRGWFKS SLLTAVVARD GKPPYKAVLT HGFTVDEQGR 600
KMSKSLGNVV DPADVIKRYG ADILRLWVAS TDYRHDMALS ENILKQVADA 650
YRKIRNTLRY LLGNLYDFNP DTDMVERDDL LEIDRWQMHR LQEVIRKVTE 700
AYREYEYHIV YHTLNNYCAV DLSAVYLDIL KDRLYTSAPA SRERRSAQTV 750
LYHVADALIR MLTPILTFTA EEAYSHLPKP AGSPPTSQLL MMPQPDPAYL 800
DENLAAEWDR LMELRDAVQV VLERARVDKL IGSSQEAAVN LYASGGEGSW 850
AELLDRHLPD LPSIFIVSDV KLFVGGQSAP SGTYFGEGPG DLSVEVVRAE 900
GEKCERCWNY RKVGAIEQHP TLCERCAGVV LSLNLDNA 938
Length:938
Mass (Da):106,189
Last modified:October 11, 2004 - v1
Checksum:iA21A5E420CA8C381
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006840 Genomic DNA. Translation: BAD40216.1.
RefSeqiYP_075060.1. NC_006177.1.

Genome annotation databases

EnsemblBacteriaiBAD40216; BAD40216; STH1231.
GeneIDi2979836.
KEGGisth:STH1231.
PATRICi23780624. VBISymThe116959_1233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006840 Genomic DNA. Translation: BAD40216.1 .
RefSeqi YP_075060.1. NC_006177.1.

3D structure databases

ProteinModelPortali Q67Q27.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 292459.STH1231.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD40216 ; BAD40216 ; STH1231 .
GeneIDi 2979836.
KEGGi sth:STH1231.
PATRICi 23780624. VBISymThe116959_1233.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci STHE292459:GJMM-1300-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
    Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
    Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T / IAM 14863.

Entry informationi

Entry nameiSYI_SYMTH
AccessioniPrimary (citable) accession number: Q67Q27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi