Q67PC7 (Q67PC7_SYMTH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

STH1481

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q67PC7 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2C0A3C19F04B345F	FASTA	735	83,210
	10 20 30 40 50 60 MPKSLIIVES PAKAKTIEKF LGRKYAVKAS MGHVRDLPKS QLGVSVENDF EPKYITIRGK 70 80 90 100 110 120 GDILKELRES ARKADRVYLA TDPDREGEAI SWHLAQVLKL PLDEPLRIEF HEITKDAIQR 130 140 150 160 170 180 AIKQPRPIDL NRVEAQQARR VLDRLVGYKL SPLLWRKVRR GLSAGRVQSV AVRLIVDRER 190 200 210 220 230 240 EIQAFQPEEY WTLDARLSTA FAQSFSARYW GMGEQKADLK TQDQVRAVVH QVAGDRLPAG 250 260 270 280 290 300 NAAVSGEGTP GDPFTEVLDL AGEGLVLQVR SVKRREKKRN PAYPFTTSSL QQEASRKLGF 310 320 330 340 350 360 SVRRTMAVAQ QLYEGLPLGE EGHTGLVTYI RTDSTRIADE AARAAAEFIE RQYGKDYVPE 370 380 390 400 410 420 RRREPDRRAG EQGAHEAIRP TDVTRTPESV KPYLTPDQYR LYRLIWERFL ASQMAPAVLD 430 440 450 460 470 480 TVTVELVAGE HVFRASGQSI RFPGFMKVYI EGEDDDGQRE EGDDLLPELA EGQQVTLQTL 490 500 510 520 530 540 EARQHFTQPP PRYSEAMLVK ALEERGIGRP STYAPIIGTI QTRGYVEKRD KRFYPTELGV 550 560 570 580 590 600 LVTDILKEYF PDIIDVEFTA HLEGKLDEVE EGRVNWRELI RRFYGPFEET LKQAEEKVGG 610 620 630 640 650 660 FELEDEVSDV PCEKCGRLMV VKHGRFGKFL ACPGFPECKS TKPILEETGV TCPACGTGRI 670 680 690 700 710 720 VERKSKKGGR RFYGCTNYPD CNFVSWEKPV NRLCPECGAP YLVEKRIKEL GLSHVCKQPD 730 CGYVEPVESM EEVHA

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD40466.1.
RefSeq	YP_075310.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q67PC7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2979454.
GenomeReviews	Gene locus STH1481 in contig AP006840_GR.
KEGG	sth:STH1481.
NMPDR	fig|292459.1.peg.1422.
PATRIC	23781115. VBISymThe116959_1478.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
Enzyme and pathway databases
BioCyc	STHE292459:STH1481-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 3 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q67PC7_SYMTH

Accession

Primary (citable) accession number: Q67PC7

Entry history

Integrated into UniProtKB/TrEMBL:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)