Reviewed,
UniProtKB/Swiss-Prot Q67NM2 (LEXA_SYMTH)
Last modified
March 2, 2010.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: LexA repressor EC=3.4.21.88 | ||||
| Gene names |
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| Organism | Symbiobacterium thermophilum [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 2734 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium |
Protein attributes
| Sequence length | 205 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015 |
| Catalytic activity | Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015 |
| Subunit structure | Homodimer By similarity. HAMAP MF_00015 |
| Sequence similarities | Belongs to the peptidase S24 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation |
| Ligand | DNA-binding |
| Molecular function | Hydrolase Repressor |
| PTM | Autocatalytic cleavage |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: HAMAP SOS responseInferred from electronic annotation. Source: HAMAP cellular DNA replicationInferred from electronic annotation. Source: HAMAP cellular transcriptionInferred from electronic annotation. Source: UniProtKB-KW negative regulation of cellular transcription, DNA-dependentInferred from electronic annotation. Source: HAMAP proteolysisInferred from electronic annotation. Source: InterPro |
| Molecular function | DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 205 | 205 | LexA repressor HAMAP MF_00015 | PRO_0000170097 | |||||
Regions | |||||||||
| DNA binding | 29 – 49 | 21 | H-T-H motif By similarity | ||||||
Sites | |||||||||
| Active site | 128 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Active site | 165 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Site | 91 – 92 | 2 | Cleavage; by autolysis By similarity | ||||||
Sequences
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References
| [1] | "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AP006840 Genomic DNA. Translation: BAD40721.1. |
| RefSeq | YP_075565.1. |
3D structure databases | |
| SMR | Q67NM2. Positions 82-205. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S24.001. |
Genome annotation databases | |
| GeneID | 2979854. |
| GenomeReviews | Gene locus STH1736 in contig AP006840_GR. |
| KEGG | sth:STH1736. |
| NMPDR | fig|292459.1.peg.1674. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG679610. |
| OMA | KVIGVFR. |
| ProtClustDB | PRK00215. |
Enzyme and pathway databases | |
| BioCyc | STHE292459:STH1736-MONOMER. |
| BRENDA | 3.4.21.88. 20615. |
Family and domain databases | |
| HAMAP | MF_00015. LexA. [Tree] |
| InterPro | IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view] |
| PRINTS | PR00726. LEXASERPTASE. |
| SUPFAM | SSF51306. Pept_S24_S26_C. 1 hit. |
| TIGRFAMs | TIGR00498. lexA. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | LEXA_SYMTH | ||||||||
| Accession | Primary (citable) accession number: Q67NM2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
