Q67NB6 (DXS_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	STH1842

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256490

Sequences

	Sequence		Length	Mass (Da)
	Q67NB6 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 236B8497DA0A6D00	FASTA	648	69,789
	10 20 30 40 50 60 MHLRDLTGPE QLKRLAPAEL AQLAAEIRRV ILETVATNGG HLAPNLGVVE LTLALHIVFD 70 80 90 100 110 120 SPRDKILWDV SHQSYVHKLL TGRLHQFHTL RQFGGIAGFT DPRESVHDHF HWGHASTSIS 130 140 150 160 170 180 AAVGMAKARD LAGDDYEVVA VIGDGALTGG MAYEALDHAG HDKTKVIVVL NDNSMSIAPN 190 200 210 220 230 240 VGGISNYLAR IRTGPSYQRV KHDVAEALRQ IPLIGPQALE LADRLKEGVK HLLVHNMFFE 250 260 270 280 290 300 DLGFTYLGPV DGHNVSALVD VLRQARAYPG PTVVHVVTTK GKGVPYAEQL PDKFHGGGPF 310 320 330 340 350 360 DVATGRTGPG SLTYSEVFGN VMCKLAAEDP RVCAITAAMP SGTGLSRFAR QFPDRYFDVG 370 380 390 400 410 420 IAEQHAVTFA AGLAKGGMRP VFAVYSTFLQ RAYDQVIHDV ALQNLPVTLA IDRGGLVEDG 430 440 450 460 470 480 ATHQGVFDVA YLRAIPNMVV MAPKDENELQ HMLYTALCHD GPAALRYPRG KAQGVPLDET 490 500 510 520 530 540 LQPLPIGRGE VMQEGADVAL IGLGTMARVC QEAARLLAEK SISAMVINPR FVKPLDAELL 550 560 570 580 590 600 LRAGREVGAV VTVEEACLAG GFGSAVLELY AAHGVNARVE RMGIPDEFVD HGQPARYLER 610 620 630 640 YGLTPEGVAQ RAEALLLRMR SDLAAQPARR SRSVRRLSGA KAAGNGET

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD40827.1.
RefSeq	YP_075671.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q67NB6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2981876.
GenomeReviews	Gene locus STH1842 in contig AP006840_GR.
KEGG	sth:STH1842.
NMPDR	fig|292459.1.peg.1777.
PATRIC	23781845. VBISymThe116959_1842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RFANEHD.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	STHE292459:STH1842-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_SYMTH

Accession

Primary (citable) accession number: Q67NB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program