Reviewed, UniProtKB/Swiss-Prot Q67N73 (DNAE2_SYMTH)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	STH1885

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1085	1085	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103400

Sequences

	Sequence		Length	Mass (Da)
	Q67N73-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 1BC11470BF0DB77E	FASTA	1,085	120,631
	10 20 30 40 50 60 MSFVHLHVHS PFSFLDGASR LDDLIAEAAL HGMPALALTD HNNVSGAVRF QRKAQEAGIQ 70 80 90 100 110 120 PIQGAELTLE GGYHLTLLAT GPKGYANLNR LITAAHLGQE PRATPGAMFA LAPGHDRLQP 130 140 150 160 170 180 ACPLSALSHD TDGLIALSGC RRGEIPSLIL RGRLAEAEAA ARRYAAWFPG RFYLELHDER 190 200 210 220 230 240 LPGEQALNRA LRDLGEALGL PLVVTSNVHH RTREEFFVHD LLTCVRTLTR IDQPHPERPL 250 260 270 280 290 300 NDQRYLKPPE VMIERFREFP EAVKNTLAIA ERCRPVLDLD ARLHPEFQTP DGSHPAAYLT 310 320 330 340 350 360 HLVYDGAARR YGRITERIRR RLEHELTIIT RLGYEDYFLL VWDVVRFARE RGIRCAGRGS 370 380 390 400 410 420 AADSAVAYCL FITDVDAIER GLLFERFMSL ERAQKPDIDI DFDARRRDEV AAYVYRKYGA 430 440 450 460 470 480 DHVASVCTYN TFQARSAVRD LGKAMGFQEA DLDYLAKRIP WHAGADEILT VMARYPELRQ 490 500 510 520 530 540 SGIPWHKFEQ LVQAAERVAR FPRFIGTHLG GLVISRRPLL EVTPLQMAAK GQVVCQFDKE 550 560 570 580 590 600 YVEDLGLVKL DLLSLRTFTA VDDAVRAIGD IDYEQIPHGD AATYEMLGVG ESIGVFQLES 610 620 630 640 650 660 PAQRALQARL KPDRFEDIVA SVAIIRPGPI KGNMVEPFLA RRHGKEEVTY LHPKLKPILE 670 680 690 700 710 720 KTYGVVLFQE QVIEIATAVA GFTPGEADQL RRVMTHARNR EDMEAIGRLF RQKAAAAGVD 730 740 750 760 770 780 PAVADTIFSY IQGYASYGFC EAHAAAFANT AYKTAYLVRH YPAEYFAALM SAQPMGYYPI 790 800 810 820 830 840 NTLAVEARRR GVGLLPVDIN RSEEAFTVEE WSREAWEAFW GMEPFAPEYP ATGKAIRIGL 850 860 870 880 890 900 RAVKGVGEPA AAAILAARDG GEFRSLADLV RRTGGAVPRG PLEALVLAGA FDRLHSNRRA 910 920 930 940 950 960 ALWQVAHLLE AERIRQERGA GQAGLLDGDA PGLGAQAAEA LEAASPQGTP AIADFGAAER 970 980 990 1000 1010 1020 YLKEYELTGL MVRTHFMRFV RERLAREGYL TAQEVRQRRA GELVKVAGLP VCPHRPPTRS 1030 1040 1050 1060 1070 1080 GKIVVFLSLE DETGLIDLTI FEDVYQRYGH LIFTDPRPPL AALGRVDRRG GHVSITVNRL RLLDT

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006840 Genomic DNA. Translation: BAD40870.1.
RefSeq	YP_075714.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2980226.
GenomeReviews	Gene locus STH1885 in contig AP006840_GR.
KEGG	sth:STH1885.
NMPDR	fig|292459.1.peg.1819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q67N73.
OMA	YGRITER.
Enzyme and pathway databases
BioCyc	STHE292459:STH1885-MON.
BRENDA	2.7.7.7. 20615.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_SYMTH

Accession

Primary (citable) accession number: Q67N73

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)