Q67LP5 (KATG_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	STH2416

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Catalase-peroxidase HAMAP MF_01961		PRO_0000354940
Sites
	Active site	89	1	Proton acceptor By similarity
	Metal binding	252	1	Iron (heme axial ligand) By similarity
	Site	85	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	88 ↔ 211		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-237) By similarity
	Cross-link	211 ↔ 237		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-88) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67LP5 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 951B04CA6355A898	FASTA	725	81,957
	10 20 30 40 50 60 MREEDIRSTA GHGTSNKDWW PNQLNLRILH QHSEKANPMG PDFNYREEFK KLDYWALKED 70 80 90 100 110 120 LRKLMTESQD WWPADFGHYG PLIIRMAWHS AGTYRIQDGR GGAESGAQRF APLNSWPDNI 130 140 150 160 170 180 NLDKARRLLW PIKQKYGRRI SWADLMILAG NVALESMGLK TIGFAGGRAD VWEPEEDIYW 190 200 210 220 230 240 GSEQQWLGRD RFGEEGKLED PLAASEMGLI YVNPEGPGRE PDPLKAAQQI RETFKRMGMN 250 260 270 280 290 300 DEETVALIAG GHTFGKTHGA ASPSHLGPEP EAAPIEEMGL GWKNSYGTGK GGDTITSGLE 310 320 330 340 350 360 VTWTSSPTKW TSNFLWNLFG YEWELTKSPA GAWQWRPKNG AGEGTVPDAH DPNKRHAPGM 370 380 390 400 410 420 LTTDIALRVD PVYEKIARRF LENPDEFAKA FARAWFKLTH RDLGPRSRYL GPEVPEEEFI 430 440 450 460 470 480 WQDPLPKRDY DLIDEGDIAE LKKRIQASGM SIREMVMTAW ASASTFRGSD KRGGANGARI 490 500 510 520 530 540 RLAPQIGWEV NEPEQLRPVL ETLEGIQQEF NRSQTGRKRV SLADLIVLAG CVGIEQAARN 550 560 570 580 590 600 AGFEITVPFT PGRVDATQEQ TDVESFSYLE PVHDGFRNYL KRKFSVPAEH LLIDRANLLT 610 620 630 640 650 660 LTAPEMTVLI GGLRVLDCNW GRTKHGVLTD RPGALTNDFF VNLLDMRWKW NATDDENVFE 670 680 690 700 710 720 GRDRATGELK WTATRVDLIF GSNAQLRAIA EVYASNDGQE KFVQDFVKAW TKVMNLDRFD LLVKK

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD41401.1.
RefSeq	YP_076245.1. NC_006177.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q67LP5.
SMR	Q67LP5. Positions 11-721.
ModBase	Search...
Protein family/group databases
PeroxiBase	2350. SthCP01_IAM14863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2980843.
GenomeReviews	Gene locus STH2416 in contig AP006840_GR.
KEGG	sth:STH2416.
NMPDR	fig|292459.1.peg.2313.
PATRIC	23783002. VBISymThe116959_2412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	STHE292459:STH2416-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SYMTH

Accession

Primary (citable) accession number: Q67LP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program