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Q67LB8 (MDH_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:STH2543
OrganismSymbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]
Taxonomic identifier292459 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113474

Regions

Nucleotide binding10 – 156NAD By similarity
Nucleotide binding121 – 1233NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site341NAD By similarity
Binding site851Substrate By similarity
Binding site911Substrate By similarity
Binding site981NAD By similarity
Binding site1231Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q67LB8 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 8277B3A4A25CDF1A

FASTA31533,229
        10         20         30         40         50         60 
MKRKKIAIVG SGFTGQGTAL FAAARDLGDI VLVDLPAREN YAKGVALDMM EAMPVYGSDT 

        70         80         90        100        110        120 
RLVGTSDYAE IAGADVVVIT AGVPRKPGMS REDLVNTNAG IVKDVAEKVA RYAPDSVIIV 

       130        140        150        160        170        180 
LTNPVDSMTY VALKASGFPK NRVIGQSGVL DTARFRTFLA NAIGCSFQDV VGCVLGGHGD 

       190        200        210        220        230        240 
DMVPLVRYTS AGGIPVEKLL PKETIDAIVE RTRKGGGEIV NLMGTSAGYA PGMALVEMID 

       250        260        270        280        290        300 
AILNDRKRIL PSIAYLEGEY GYTDMCLGVM TVLGGGGLEK VIELDLTDEE KVALDKGAQG 

       310 
VRNLIEMLKA GILAQ 

« Hide

References

[1]"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T / IAM 14863.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006840 Genomic DNA. Translation: BAD41528.1.
RefSeqYP_076372.1. NC_006177.1.

3D structure databases

ProteinModelPortalQ67LB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292459.STH2543.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD41528; BAD41528; STH2543.
GeneID2979396.
KEGGsth:STH2543.
PATRIC23783256. VBISymThe116959_2536.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAYGQNDIC.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycSTHE292459:GJMM-2624-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_SYMTH
AccessionPrimary (citable) accession number: Q67LB8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families