Q67KX6 (ILVD_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	STH2685

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225430
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q67KX6 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F5E5B2479EC6E4F3	FASTA	565	59,431
	10 20 30 40 50 60 MEGSTVRSDL IKKGPDRAPH RALLKATGVT DADMGKPFIG IANSYIDIVP GHVHLKEFGD 70 80 90 100 110 120 LVKEAVREAG GVPFIFNTIG VDDGIAMGHI GMRYSLPSRE LIADAVETVI EAHQLDALIC 130 140 150 160 170 180 IPNCDKITPG MLMAAMRVNI PTIFISGGPM AAGRLKDGRR ADLITIFEGV GAHSRGMISD 190 200 210 220 230 240 EELKELEDLA CPSCGSCSGM FTANSMNCLC EALGLALPGN GTALARSAER EALARAAARR 250 260 270 280 290 300 IVEMALAGGP RPRDIVTPEA IDNAFALDIA MGGSTNTVLH TLAVATEAGI AYPLERINAL 310 320 330 340 350 360 SARVPYLCKV SPATAEVHIE DVHAAGGVMA ILKELSRKEG TLHPHCLTVT GKTIGELWAE 370 380 390 400 410 420 AENRNREVIR PVEDPYAETG GLAILFGNLA PDGAALKVGA VDPSITTFTG RAICFDSQDE 430 440 450 460 470 480 ALQGIFGGRV QPGHVVVIRY EGPKGGPGMP EMLAPTSAIV GMGLGKEVAL ITDGRFSGGT 490 500 510 520 530 540 RGICLGHISP EAAEGGPIAL VRDGDVITID IPGRRLTLEV PADELERRRA AWVKPAPKVR 550 560 KGWLGRYAAL VTSANTGAVL RTPEE

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD41670.1.
RefSeq	YP_076514.1. NC_006177.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2980170.
GenomeReviews	Gene locus STH2685 in contig AP006840_GR.
KEGG	sth:STH2685.
NMPDR	fig|292459.1.peg.2573.
PATRIC	23783546. VBISymThe116959_2678.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
Enzyme and pathway databases
BioCyc	STHE292459:STH2685-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_SYMTH

Accession

Primary (citable) accession number: Q67KX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program