Q67KF8 (PURL_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	STH2855

Organism

Symbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]

Taxonomic identifier

292459 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales incertae sedis › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium › Symbiobacterium thermophilum

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 778	778	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100495
Regions
	Nucleotide binding	101 – 112	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q67KF8 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: BEC34D07094EADC4	FASTA	778	82,896
	10 20 30 40 50 60 MSEMQQPWSL VGLTEAEYRR VVEILGREPN PTELHMFGVM WSEHCAYKHS KAALRRLPTR 70 80 90 100 110 120 GEHVLQGPGE NAGVVRIDDD LAVAFKLESH NHPSFVDPFN GAATGVGGIL RDVFTMGARP 130 140 150 160 170 180 VATLNSLRFG PLDEPKQREL LRGVVAGIGH YGNTVGVPCI GGEVYFDESY RGNCLVNAMC 190 200 210 220 230 240 IGILRPDRIH RGIAAGPGNA IMVVGNPTGR DGIHAASLLA SAEFSGSEAE AALPTVPVGD 250 260 270 280 290 300 PFAEKMLMEA CLELFETDAV VGIQDMGAAG LISSSSEMAA RGGVGVELDV RKVPAREEGM 310 320 330 340 350 360 EPWEFLLSES QERMLVCVKK GREAEVEAIC RKWGVGCAVI GRVTDDGMVR VLDDGRVVAE 370 380 390 400 410 420 VPARALAEAP VYHPAKAEPA YLAELRAFDW SRLPEPEDWN ETLLRLLGSP NIGARAWIYE 430 440 450 460 470 480 QFDHMVQAGT VLGPGGDAGV IRLGAAVAPG AHGLTRQPPG EGLPGRSGQA GPPKGIAASV 490 500 510 520 530 540 DCNGRYVYLN PRRGTAIAVA EAARNCVVTG ARPVAITNNC NFGNPEKPEI FWTFDEAITG 550 560 570 580 590 600 MAEACEALGT PVTGGNVSFY NETSGEAIHP TPTIGMIAVH ENLDRLTTPG FKQVGDVILL 610 620 630 640 650 660 LGETRDELGG SEYARLIHGV LAGDAPALDL AFEKRLQDVV LRAIHEGLVT AAHDVAEGGL 670 680 690 700 710 720 AVALAEMAIA AAEPSLGCQV SIFLGEGRVD GQLFGESQSR ILVTATREQV GRLQALLMVE 730 740 750 760 770 QIPFRVLGDV TADGRFRLAA LAPGSGSAHV YRRQELIDLP VADLVRAHKE AIPRWMDA

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD41840.1.
RefSeq	YP_076684.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q67KF8.
ModBase	Search...
Protein-protein interaction databases
STRING	292459.STH2855.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD41840; BAD41840; STH2855.
GeneID	2979754.
KEGG	sth:STH2855.
PATRIC	23783891. VBISymThe116959_2834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_SYMTH

Accession

Primary (citable) accession number: Q67KF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program