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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-3293-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:STH3189
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSymbiobacteriaceaeSymbiobacterium
ProteomesiUP000000417 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000199401Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi292459.STH3189.

Structurei

3D structure databases

ProteinModelPortaliQ67JH9.
SMRiQ67JH9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q67JH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSDIAIAQR ARLEPITKVA EQIGLGPEDL ELYGRYKAKV ADHVWPRVRS
60 70 80 90 100
NPDGKLILVT AISPTPAGEG KTTVTVGLGQ AMSRIGKRAI IALREPSLGP
110 120 130 140 150
AFGVKGGAAG GGYSQVVPMD EINLHFTGDF HAVTAANNLL AAMIDNHLHQ
160 170 180 190 200
GNKLGLDPRQ ITFKRVLDMN DRALRSVVIG LGGKNGGVPR QEEFMITPAS
210 220 230 240 250
EVMATLCLAE DLADLKRRCG EIIVGYTYDG APVRARDLKA EGAMATLLKE
260 270 280 290 300
AIKPNLVQTL ENTPAFVHGG PFANIAHGCN TVVATRLALK LADYVITEAG
310 320 330 340 350
FGADLGAEKF IDIKCRLSGL RPDAVVVVAT IRSLKMHGGL KKDDLAREDL
360 370 380 390 400
EALQRGSANL MRHLRNVTEV FGLPAVVAIN RFAADTEAEI ALLRALVEEA
410 420 430 440 450
GATAVVADVH ARGGDGGIEL AQRVVELVEQ PNRFRFAYDD EDSLKTKIEK
460 470 480 490 500
VATRIYGADG VDFTREASRM LKKLESEGFG RAPVCIAKTQ YSFSDDPKKL
510 520 530 540 550
GAPTGWRLTV REVRPSAGAG FVVALTGEIM TMPGLPPVPA AESIDVSDDG

EITGLF
Length:556
Mass (Da):59,450
Last modified:October 10, 2004 - v1
Checksum:i49822EE6AAD9BDAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD42171.1.
RefSeqiYP_077015.1. NC_006177.1.

Genome annotation databases

EnsemblBacteriaiBAD42171; BAD42171; STH3189.
GeneIDi2981679.
KEGGisth:STH3189.
PATRICi23784579. VBISymThe116959_3173.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD42171.1.
RefSeqiYP_077015.1. NC_006177.1.

3D structure databases

ProteinModelPortaliQ67JH9.
SMRiQ67JH9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292459.STH3189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD42171; BAD42171; STH3189.
GeneIDi2981679.
KEGGisth:STH3189.
PATRICi23784579. VBISymThe116959_3173.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSTHE292459:GJMM-3293-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
    Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
    Nucleic Acids Res. 32:4937-4944(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T / IAM 14863.

Entry informationi

Entry nameiFTHS_SYMTH
AccessioniPrimary (citable) accession number: Q67JH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2005
Last sequence update: October 10, 2004
Last modified: January 6, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.