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Q67JH9 (FTHS_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:STH3189
OrganismSymbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]
Taxonomic identifier292459 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199401

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q67JH9 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 49822EE6AAD9BDAE

FASTA55659,450
        10         20         30         40         50         60 
MLSDIAIAQR ARLEPITKVA EQIGLGPEDL ELYGRYKAKV ADHVWPRVRS NPDGKLILVT 

        70         80         90        100        110        120 
AISPTPAGEG KTTVTVGLGQ AMSRIGKRAI IALREPSLGP AFGVKGGAAG GGYSQVVPMD 

       130        140        150        160        170        180 
EINLHFTGDF HAVTAANNLL AAMIDNHLHQ GNKLGLDPRQ ITFKRVLDMN DRALRSVVIG 

       190        200        210        220        230        240 
LGGKNGGVPR QEEFMITPAS EVMATLCLAE DLADLKRRCG EIIVGYTYDG APVRARDLKA 

       250        260        270        280        290        300 
EGAMATLLKE AIKPNLVQTL ENTPAFVHGG PFANIAHGCN TVVATRLALK LADYVITEAG 

       310        320        330        340        350        360 
FGADLGAEKF IDIKCRLSGL RPDAVVVVAT IRSLKMHGGL KKDDLAREDL EALQRGSANL 

       370        380        390        400        410        420 
MRHLRNVTEV FGLPAVVAIN RFAADTEAEI ALLRALVEEA GATAVVADVH ARGGDGGIEL 

       430        440        450        460        470        480 
AQRVVELVEQ PNRFRFAYDD EDSLKTKIEK VATRIYGADG VDFTREASRM LKKLESEGFG 

       490        500        510        520        530        540 
RAPVCIAKTQ YSFSDDPKKL GAPTGWRLTV REVRPSAGAG FVVALTGEIM TMPGLPPVPA 

       550 
AESIDVSDDG EITGLF 

« Hide

References

[1]"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T / IAM 14863.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006840 Genomic DNA. Translation: BAD42171.1.
RefSeqYP_077015.1. NC_006177.1.

3D structure databases

ProteinModelPortalQ67JH9.
SMRQ67JH9. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292459.STH3189.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD42171; BAD42171; STH3189.
GeneID2981679.
KEGGsth:STH3189.
PATRIC23784579. VBISymThe116959_3173.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMAIANSVSM.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycSTHE292459:GJMM-3293-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_SYMTH
AccessionPrimary (citable) accession number: Q67JH9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways