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Q67GH9 (LCPS_BLATR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

  1. Lycopene beta-cyclase
    EC=5.5.1.19
    Alternative name(s):
    Lycopene cyclase
  2. Phytoene synthase
    EC=2.5.1.32
Gene names
Name:carRA
OrganismBlakeslea trispora (Choanephora trispora)
Taxonomic identifier4850 [NCBI]
Taxonomic lineageEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeChoanephoraceaeChoanephoroideaeBlakeslea

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase). Ref.1

Catalytic activity

Carotenoid psi-end group = carotenoid beta-end group.

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; beta-carotene biosynthesis.

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Induction

Strongly induced during mating of two strains of the opposite sex. Ref.2

Biotechnological use

Beta-carotene is a popular additive for butter, ice cream, orange juice and candies in the food industry. B.trispora is one of the microorganisms of choice for industrial production of this molecule. Ref.1

Sequence similarities

In the N-terminal section; belongs to the lycopene beta-cyclase family.

In the C-terminal section; belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Bifunctional lycopene cyclase/phytoene synthase
PRO_0000409234

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane37 – 5620Helical; Potential
Transmembrane80 – 9718Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane150 – 17021Helical; Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane218 – 23821Helical; Potential
Region1 – 240240Lycopene beta-cyclase
Region247 – 608362Phytoene synthase

Natural variations

Natural variant1431P → S in strain: F-744 / L25; beta-carotene overproducing strain. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q67GH9 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 346A3950D9DC459D

FASTA60869,592
        10         20         30         40         50         60 
MSILTYLEFH LYYTLPVLAA LCWLLKPFHS QQDNLKYKFL MLMAASTASI WDNYIVYHRA 

        70         80         90        100        110        120 
WWYCPTCVVA VIGYVPLEEY MFFIIMTLMT VAFSNFVMRW HLHTFFIRPN TSWKQTLLVR 

       130        140        150        160        170        180 
LVPVSALLAI TYHAWHLTLP NKPSFYGSCI LWYACPVLAI LWLGAGEYIL RRPVAVLLSI 

       190        200        210        220        230        240 
VIPSVYLCWA DIVAISAGTW HISLRTSTGK MVVPDLPVEE CLFFTLINTV LVFATCAIDR 

       250        260        270        280        290        300 
AQAILHLYKS SVQNQNPKQA ISLFQHVKEL AWAFCLPDQM LNNELFDDLT ISWDILRKAS 

       310        320        330        340        350        360 
KSFYTASAVF PSYVRQDLGV LYAFCRATDD LCDDESKSVQ ERRDQLDLTR QFVRDLFSQK 

       370        380        390        400        410        420 
TSAPIVIDWE LYQNQLPASC ISAFRAFTRL RHVLEVDPVE ELLDGYKWDL ERRPILDEQD 

       430        440        450        460        470        480 
LEAYSACVAS SVGEMCTRVI LAQDQKENDA WIIDRAREMG LVLQYVNIAR DIVTDSETLG 

       490        500        510        520        530        540 
RCYLPQQWLR KEETEQIQQG NARSLGDQRL LGLSLKLVGK ADAIMVRAKK GIDKLPANCQ 

       550        560        570        580        590        600 
GGVRAACQVY AAIGSVLKQQ KTTYPTRAHL KGSERAKIAL LSVYNLYQSE DKPVALRQAR 


KIKSFFVD 

« Hide

References

[1]"Blakeslea trispora genes for carotene biosynthesis."
Rodriguez-Saiz M., Paz B., De La Fuente J.L., Lopez-Nieto M.J., Cabri W., Barredo J.L.
Appl. Environ. Microbiol. 70:5589-5594(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-143, FUNCTION, BIOTECHNOLOGY.
Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309 and F-744 / L25.
[2]"Analysis of mating-dependent transcription of Blakeslea trispora carotenoid biosynthesis genes carB and carRA by quantitative real-time PCR."
Schmidt A.D., Heinekamp T., Matuschek M., Liebmann B., Bollschweiler C., Brakhage A.A.
Appl. Microbiol. Biotechnol. 67:549-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY176662 Genomic DNA. Translation: AAO46893.1.
AY176663 Genomic DNA. Translation: AAO46895.1.
AY884174 Genomic DNA. Translation: AAX20904.1.

3D structure databases

ProteinModelPortalQ67GH9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR03462. CarR_dom_SF. 2 hits.
PROSITEPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCPS_BLATR
AccessionPrimary (citable) accession number: Q67GH9
Secondary accession number(s): Q67GI1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways