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Q67GH9

- LCPS_BLATR

UniProt

Q67GH9 - LCPS_BLATR

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Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

carRA

Organism
Blakeslea trispora (Choanephora trispora)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
  2. intramolecular lyase activity Source: InterPro
  3. lycopene beta cyclase activity Source: UniProtKB
  4. phytoene synthase activity Source: UniProtKB

GO - Biological processi

  1. carotene biosynthetic process Source: UniProtKB
  2. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
Name:carRA
OrganismiBlakeslea trispora (Choanephora trispora)
Taxonomic identifieri4850 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeChoanephoraceaeChoanephoroideaeBlakeslea

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Biotechnological usei

Beta-carotene is a popular additive for butter, ice cream, orange juice and candies in the food industry. B.trispora is one of the microorganisms of choice for industrial production of this molecule.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Bifunctional lycopene cyclase/phytoene synthasePRO_0000409234Add
BLAST

Expressioni

Inductioni

Strongly induced during mating of two strains of the opposite sex.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ67GH9.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence AnalysisAdd
BLAST
Transmembranei37 – 5620HelicalSequence AnalysisAdd
BLAST
Transmembranei80 – 9718HelicalSequence AnalysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 240240Lycopene beta-cyclaseAdd
BLAST
Regioni247 – 608362Phytoene synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q67GH9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSILTYLEFH LYYTLPVLAA LCWLLKPFHS QQDNLKYKFL MLMAASTASI
60 70 80 90 100
WDNYIVYHRA WWYCPTCVVA VIGYVPLEEY MFFIIMTLMT VAFSNFVMRW
110 120 130 140 150
HLHTFFIRPN TSWKQTLLVR LVPVSALLAI TYHAWHLTLP NKPSFYGSCI
160 170 180 190 200
LWYACPVLAI LWLGAGEYIL RRPVAVLLSI VIPSVYLCWA DIVAISAGTW
210 220 230 240 250
HISLRTSTGK MVVPDLPVEE CLFFTLINTV LVFATCAIDR AQAILHLYKS
260 270 280 290 300
SVQNQNPKQA ISLFQHVKEL AWAFCLPDQM LNNELFDDLT ISWDILRKAS
310 320 330 340 350
KSFYTASAVF PSYVRQDLGV LYAFCRATDD LCDDESKSVQ ERRDQLDLTR
360 370 380 390 400
QFVRDLFSQK TSAPIVIDWE LYQNQLPASC ISAFRAFTRL RHVLEVDPVE
410 420 430 440 450
ELLDGYKWDL ERRPILDEQD LEAYSACVAS SVGEMCTRVI LAQDQKENDA
460 470 480 490 500
WIIDRAREMG LVLQYVNIAR DIVTDSETLG RCYLPQQWLR KEETEQIQQG
510 520 530 540 550
NARSLGDQRL LGLSLKLVGK ADAIMVRAKK GIDKLPANCQ GGVRAACQVY
560 570 580 590 600
AAIGSVLKQQ KTTYPTRAHL KGSERAKIAL LSVYNLYQSE DKPVALRQAR

KIKSFFVD
Length:608
Mass (Da):69,592
Last modified:October 11, 2004 - v1
Checksum:i346A3950D9DC459D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti143 – 1431P → S in strain: F-744 / L25; beta-carotene overproducing strain. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY176662 Genomic DNA. Translation: AAO46893.1.
AY176663 Genomic DNA. Translation: AAO46895.1.
AY884174 Genomic DNA. Translation: AAX20904.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY176662 Genomic DNA. Translation: AAO46893.1 .
AY176663 Genomic DNA. Translation: AAO46895.1 .
AY884174 Genomic DNA. Translation: AAX20904.1 .

3D structure databases

ProteinModelPortali Q67GH9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00799 ; UER00773 .
UPA00802 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
PROSITEi PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-143, FUNCTION, BIOTECHNOLOGY.
    Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309 and F-744 / L25.
  2. "Analysis of mating-dependent transcription of Blakeslea trispora carotenoid biosynthesis genes carB and carRA by quantitative real-time PCR."
    Schmidt A.D., Heinekamp T., Matuschek M., Liebmann B., Bollschweiler C., Brakhage A.A.
    Appl. Microbiol. Biotechnol. 67:549-555(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309.

Entry informationi

Entry nameiLCPS_BLATR
AccessioniPrimary (citable) accession number: Q67GH9
Secondary accession number(s): Q67GI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 11, 2004
Last modified: October 1, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3