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Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

carRA

Organism
Blakeslea trispora (Choanephora trispora)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi: beta-carotene biosynthesis

This protein is involved in the pathway beta-carotene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway beta-carotene biosynthesis and in Carotenoid biosynthesis.

Pathwayi: phytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional lycopene cyclase/phytoene synthase (carRA)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Transferase
Biological processCarotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773
UPA00802

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
Name:carRA
OrganismiBlakeslea trispora (Choanephora trispora)
Taxonomic identifieri4850 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotaMucoromycotinaMucoralesMucorineaeChoanephoraceaeChoanephoroideaeBlakeslea

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Transmembranei37 – 56HelicalSequence analysisAdd BLAST20
Transmembranei80 – 97HelicalSequence analysisAdd BLAST18
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Biotechnological usei

Beta-carotene is a popular additive for butter, ice cream, orange juice and candies in the food industry. B.trispora is one of the microorganisms of choice for industrial production of this molecule.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004092341 – 608Bifunctional lycopene cyclase/phytoene synthaseAdd BLAST608

Expressioni

Inductioni

Strongly induced during mating of two strains of the opposite sex.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ67GH9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 240Lycopene beta-cyclaseAdd BLAST240
Regioni247 – 608Phytoene synthaseAdd BLAST362

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR017825 Lycopene_cyclase_dom
IPR002060 Squ/phyt_synthse
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR03462 CarR_dom_SF, 2 hits
PROSITEiView protein in PROSITE
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q67GH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILTYLEFH LYYTLPVLAA LCWLLKPFHS QQDNLKYKFL MLMAASTASI
60 70 80 90 100
WDNYIVYHRA WWYCPTCVVA VIGYVPLEEY MFFIIMTLMT VAFSNFVMRW
110 120 130 140 150
HLHTFFIRPN TSWKQTLLVR LVPVSALLAI TYHAWHLTLP NKPSFYGSCI
160 170 180 190 200
LWYACPVLAI LWLGAGEYIL RRPVAVLLSI VIPSVYLCWA DIVAISAGTW
210 220 230 240 250
HISLRTSTGK MVVPDLPVEE CLFFTLINTV LVFATCAIDR AQAILHLYKS
260 270 280 290 300
SVQNQNPKQA ISLFQHVKEL AWAFCLPDQM LNNELFDDLT ISWDILRKAS
310 320 330 340 350
KSFYTASAVF PSYVRQDLGV LYAFCRATDD LCDDESKSVQ ERRDQLDLTR
360 370 380 390 400
QFVRDLFSQK TSAPIVIDWE LYQNQLPASC ISAFRAFTRL RHVLEVDPVE
410 420 430 440 450
ELLDGYKWDL ERRPILDEQD LEAYSACVAS SVGEMCTRVI LAQDQKENDA
460 470 480 490 500
WIIDRAREMG LVLQYVNIAR DIVTDSETLG RCYLPQQWLR KEETEQIQQG
510 520 530 540 550
NARSLGDQRL LGLSLKLVGK ADAIMVRAKK GIDKLPANCQ GGVRAACQVY
560 570 580 590 600
AAIGSVLKQQ KTTYPTRAHL KGSERAKIAL LSVYNLYQSE DKPVALRQAR

KIKSFFVD
Length:608
Mass (Da):69,592
Last modified:October 11, 2004 - v1
Checksum:i346A3950D9DC459D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti143P → S in strain: F-744 / L25; beta-carotene overproducing strain. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY176662 Genomic DNA Translation: AAO46893.1
AY176663 Genomic DNA Translation: AAO46895.1
AY884174 Genomic DNA Translation: AAX20904.1

Similar proteinsi

Entry informationi

Entry nameiLCPS_BLATR
AccessioniPrimary (citable) accession number: Q67GH9
Secondary accession number(s): Q67GI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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