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Q67GH9

- LCPS_BLATR

UniProt

Q67GH9 - LCPS_BLATR

Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

carRA

Organism
Blakeslea trispora (Choanephora trispora)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

    Catalytic activityi

    Carotenoid psi-end group = carotenoid beta-end group.
    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. intramolecular lyase activity Source: InterPro
    3. lycopene beta cyclase activity Source: UniProtKB
    4. phytoene synthase activity Source: UniProtKB

    GO - Biological processi

    1. carotene biosynthetic process Source: UniProtKB
    2. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00799; UER00773.
    UPA00802.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional lycopene cyclase/phytoene synthase
    Including the following 2 domains:
    Lycopene beta-cyclase (EC:5.5.1.19)
    Alternative name(s):
    Lycopene cyclase
    Phytoene synthase (EC:2.5.1.32)
    Gene namesi
    Name:carRA
    OrganismiBlakeslea trispora (Choanephora trispora)
    Taxonomic identifieri4850 [NCBI]
    Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeChoanephoraceaeChoanephoroideaeBlakeslea

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Biotechnological usei

    Beta-carotene is a popular additive for butter, ice cream, orange juice and candies in the food industry. B.trispora is one of the microorganisms of choice for industrial production of this molecule.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 608608Bifunctional lycopene cyclase/phytoene synthasePRO_0000409234Add
    BLAST

    Expressioni

    Inductioni

    Strongly induced during mating of two strains of the opposite sex.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ67GH9.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei37 – 5620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei80 – 9718HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei150 – 17021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei218 – 23821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 240240Lycopene beta-cyclaseAdd
    BLAST
    Regioni247 – 608362Phytoene synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
    In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
    PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q67GH9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSILTYLEFH LYYTLPVLAA LCWLLKPFHS QQDNLKYKFL MLMAASTASI    50
    WDNYIVYHRA WWYCPTCVVA VIGYVPLEEY MFFIIMTLMT VAFSNFVMRW 100
    HLHTFFIRPN TSWKQTLLVR LVPVSALLAI TYHAWHLTLP NKPSFYGSCI 150
    LWYACPVLAI LWLGAGEYIL RRPVAVLLSI VIPSVYLCWA DIVAISAGTW 200
    HISLRTSTGK MVVPDLPVEE CLFFTLINTV LVFATCAIDR AQAILHLYKS 250
    SVQNQNPKQA ISLFQHVKEL AWAFCLPDQM LNNELFDDLT ISWDILRKAS 300
    KSFYTASAVF PSYVRQDLGV LYAFCRATDD LCDDESKSVQ ERRDQLDLTR 350
    QFVRDLFSQK TSAPIVIDWE LYQNQLPASC ISAFRAFTRL RHVLEVDPVE 400
    ELLDGYKWDL ERRPILDEQD LEAYSACVAS SVGEMCTRVI LAQDQKENDA 450
    WIIDRAREMG LVLQYVNIAR DIVTDSETLG RCYLPQQWLR KEETEQIQQG 500
    NARSLGDQRL LGLSLKLVGK ADAIMVRAKK GIDKLPANCQ GGVRAACQVY 550
    AAIGSVLKQQ KTTYPTRAHL KGSERAKIAL LSVYNLYQSE DKPVALRQAR 600
    KIKSFFVD 608
    Length:608
    Mass (Da):69,592
    Last modified:October 11, 2004 - v1
    Checksum:i346A3950D9DC459D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti143 – 1431P → S in strain: F-744 / L25; beta-carotene overproducing strain. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY176662 Genomic DNA. Translation: AAO46893.1.
    AY176663 Genomic DNA. Translation: AAO46895.1.
    AY884174 Genomic DNA. Translation: AAX20904.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY176662 Genomic DNA. Translation: AAO46893.1 .
    AY176663 Genomic DNA. Translation: AAO46895.1 .
    AY884174 Genomic DNA. Translation: AAX20904.1 .

    3D structure databases

    ProteinModelPortali Q67GH9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .
    UPA00802 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
    PROSITEi PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-143, FUNCTION, BIOTECHNOLOGY.
      Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309 and F-744 / L25.
    2. "Analysis of mating-dependent transcription of Blakeslea trispora carotenoid biosynthesis genes carB and carRA by quantitative real-time PCR."
      Schmidt A.D., Heinekamp T., Matuschek M., Liebmann B., Bollschweiler C., Brakhage A.A.
      Appl. Microbiol. Biotechnol. 67:549-555(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: ATCC 14272 / CBS 131.59 / IMI 195169 / NRRL 2457 / QM 6309.

    Entry informationi

    Entry nameiLCPS_BLATR
    AccessioniPrimary (citable) accession number: Q67GH9
    Secondary accession number(s): Q67GI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3