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Protein

B-cell CLL/lymphoma 9-like protein

Gene

Bcl9l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1.1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: MGI

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • positive regulation of epithelial to mesenchymal transition Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of cell morphogenesis Source: MGI
  • skeletal muscle cell differentiation Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell CLL/lymphoma 9-like protein
Short name:
B-cell lymphoma 9-like protein
Short name:
BCL9-like protein
Alternative name(s):
BCL9-related beta-catenin-binding protein
Protein BCL9-2
Gene namesi
Name:Bcl9l
Synonyms:B9l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933114. Bcl9l.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14941494B-cell CLL/lymphoma 9-like proteinPRO_0000314080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei36 – 361N6-acetyllysineCombined sources
Modified residuei88 – 881PhosphoserineBy similarity
Modified residuei108 – 1081N6-acetyllysineCombined sources
Modified residuei110 – 1101N6-acetyllysineCombined sources
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei118 – 1181PhosphoserineBy similarity
Modified residuei137 – 1371N6-acetyllysineCombined sources
Modified residuei747 – 7471PhosphoserineBy similarity
Modified residuei810 – 8101PhosphoserineBy similarity
Modified residuei912 – 9121PhosphoserineCombined sources
Modified residuei923 – 9231PhosphoserineCombined sources
Modified residuei939 – 9391PhosphoserineCombined sources
Modified residuei944 – 9441PhosphoserineCombined sources
Modified residuei972 – 9721PhosphoserineBy similarity
Modified residuei984 – 9841PhosphoserineCombined sources
Modified residuei988 – 9881PhosphoserineCombined sources
Modified residuei994 – 9941PhosphoserineCombined sources
Modified residuei1001 – 10011PhosphoserineCombined sources
Modified residuei1007 – 10071PhosphoserineCombined sources
Modified residuei1014 – 10141PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ67FY2.
MaxQBiQ67FY2.
PaxDbiQ67FY2.
PRIDEiQ67FY2.

PTM databases

iPTMnetiQ67FY2.
PhosphoSiteiQ67FY2.

Expressioni

Tissue specificityi

Expressed in kidney, liver, lung, testis, brain, spleen, heart and skeletal muscle. Highly expressed in numerous colorectal tumors compared to corresponding non-cancerous tissues.1 Publication

Developmental stagei

Expressed in embryo.1 Publication

Gene expression databases

BgeeiQ67FY2.
CleanExiMM_BCL9L.
GenevisibleiQ67FY2. MM.

Interactioni

Subunit structurei

Found in a complex with CDC73; CTNNB1 and PYGO1 (By similarity). Interacts with CTNNB1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
WWOXQ9NZC73EBI-5234367,EBI-4320739From a different organism.

GO - Molecular functioni

  • beta-catenin binding Source: MGI

Protein-protein interaction databases

BioGridi219776. 1 interaction.
IntActiQ67FY2. 5 interactions.
STRINGi10090.ENSMUSP00000074516.

Structurei

3D structure databases

ProteinModelPortaliQ67FY2.
SMRiQ67FY2. Positions 238-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni302 – 530229Necessary for interaction with CTNNB1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 32447Pro-richAdd
BLAST
Compositional biasi557 – 657101Pro-richAdd
BLAST
Compositional biasi686 – 834149Met-richAdd
BLAST
Compositional biasi952 – 1373422Pro-richAdd
BLAST

Domaini

Tne C-terminal domain is important for its transactivation activity.

Sequence similaritiesi

Belongs to the BCL9 family.Curated

Phylogenomic databases

eggNOGiENOG410IEDB. Eukaryota.
ENOG410XRMX. LUCA.
GeneTreeiENSGT00730000110915.
HOGENOMiHOG000060118.
HOVERGENiHBG031116.
InParanoidiQ67FY2.
OMAiHQLMEKR.
OrthoDBiEOG7327N1.
PhylomeDBiQ67FY2.
TreeFamiTF331144.

Family and domain databases

InterProiIPR015668. Bcl-9.
IPR024670. BCL9_beta-catenin-bd_dom.
[Graphical view]
PANTHERiPTHR15185. PTHR15185. 1 hit.
PfamiPF11502. BCL9. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q67FY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRILANKTRL PHPRRREAPG SPPLSPRGHC PPAPAKPMHP ENKLTNHGKT
60 70 80 90 100
GNGGAQSQHQ NVNQGPTCNL GSKGVGAGSH GAKANQISPS NSSLKNPQAG
110 120 130 140 150
VSPFSSLKGK VKRERSVSVD SGEQREAGTP SLDSEAKEVA PRSKRRCVLE
160 170 180 190 200
RKQPYSGDEW CSGPDSEEDD KPIAAAHNCN VADPAMVTPQ LGPGQTAQLP
210 220 230 240 250
LSESSAPGPQ HGPQPGLRPD VPGGGGGGVP GKPPSQFVYV FTTHLANTAA
260 270 280 290 300
EAVLQGRAES ILAYHQQNVP RAKLDQAPKV PPTPEPLPLN TPSAGTPQSQ
310 320 330 340 350
PPPLPPPPPA PGSAPPALPP EGPPEDTSQD LAPNSVGAAS TGGGTGGTHP
360 370 380 390 400
NTPTAATANN PLPPGGDPGS APGSALLGEA TPTGNGQRNL VGSEGLSKEQ
410 420 430 440 450
LEHRERSLQT LRDIERLLLR SGETEPFLKG PPGGAGEGGP PAQAPSAAQP
460 470 480 490 500
PPSAPPGGLK KYEEPLQSMI SQTQSLGGPP LEHEVPGHPQ GGDMGQQMNM
510 520 530 540 550
MMQRLGQDSL TPEQVAWRKL QEEYYEEKRR KEEQIGLHGG RPLQDMVGMG
560 570 580 590 600
GMMGRGPPPP YHSKPGDQWP PGMGAQLRGP MDVQDPMQLR PGPPFPGPRF
610 620 630 640 650
PGNQMQRVPG FGGMQSMPME VPMNAMQRPV RPGMAWNEDL PPIGGPSNFA
660 670 680 690 700
QNAVPYPGGQ GEAERFMTPR VREELLRHQL LEKRSMGMQR PLGMAGSGMG
710 720 730 740 750
QSMEMERMIQ AHRQMDPAMF PGQMTGGDGL AGTPMGIEFG GGRGLLSPPM
760 770 780 790 800
GQSGLREVDP PMGPGNLNMN MNVNMNMNMN LNVQMTPQQQ MLMSQKMRGP
810 820 830 840 850
GDMMGPQGLS PEEMARVRAQ NSSGMMGGPQ KMLMPSQFPN QGQQGFSGGQ
860 870 880 890 900
GPYQAMPQDM GNTPDMFSPD QSSVPMGTVG TARLSHMPLP PASNPPGSVH
910 920 930 940 950
LASNRGLGRR PSDLTISINQ MGSPGMGHLK SPTLSQVHSP LVTSPSANLK
960 970 980 990 1000
SPQTPSQMVP LPSANPPGPL KSPQVLSSSL GVRSPTGSPS RLKSPSMAVP
1010 1020 1030 1040 1050
SPGWVASPKT AMPSPGVSQN KQPPLSINSS STLGNVEQGA LPPSAPRNSS
1060 1070 1080 1090 1100
SAPPANPSSG LMNPSLPFTS SPDPTPSQNP LSLMMSQMSK YAMPSSTPLY
1110 1120 1130 1140 1150
HNAIKTIATS DDELLPDRPL LPPPPPPQGS GPGISNNQPN QMHMNPAAAQ
1160 1170 1180 1190 1200
SPMGMNLPGQ QPLSHEPPPT MLPSPTPLGS NIPLHPNAQG TGGSSQNSMM
1210 1220 1230 1240 1250
MAPGGPDSLN APCGPVPSSS QMMSFPPRLQ QPHGAMAPTG AGGPGLQQHY
1260 1270 1280 1290 1300
PSGMALPPED LPTQPPGPIP PQQHLMGKGM TGRMGDAYPP GVLPGVASVL
1310 1320 1330 1340 1350
NDPELSEVIR PTPTGIPEFD LSRIIPSEKP SSTLQYFPKS ENQPPKAQPP
1360 1370 1380 1390 1400
NLHLMNLQNM MAEQTPSRPP NLPGQQGVQR GLSMSMCHPG QMSLLGRTGV
1410 1420 1430 1440 1450
PPQQGMVPHG LHQGVMSPPQ GLMTQQNFML MKQRGVGGEV YTQPPHMLSP
1460 1470 1480 1490
QGSLMGPPPQ QNLMVSHPLR QRSVSLDSQM GYLPTPGSMA NLPF
Length:1,494
Mass (Da):156,680
Last modified:October 11, 2004 - v1
Checksum:i31A9904C5923581C
GO
Isoform 2 (identifier: Q67FY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: Missing.

Show »
Length:1,457
Mass (Da):152,637
Checksum:i4FD2B47ADDE92A33
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti569 – 5702WP → CA in BAD24964 (PubMed:15574752).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3737Missing in isoform 2. 2 PublicationsVSP_030209Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128033 mRNA. Translation: BAD24964.1.
AY296058 mRNA. Translation: AAQ62696.1.
BC072555 mRNA. Translation: AAH72555.1.
BC082304 mRNA. Translation: AAH82304.1.
CCDSiCCDS40601.1. [Q67FY2-1]
RefSeqiNP_084532.2. NM_030256.2. [Q67FY2-1]
XP_006510764.1. XM_006510701.2. [Q67FY2-1]
XP_006510765.1. XM_006510702.2. [Q67FY2-1]
XP_006510766.1. XM_006510703.2. [Q67FY2-1]
XP_006510767.1. XM_006510704.2. [Q67FY2-1]
XP_006510768.1. XM_006510705.2. [Q67FY2-2]
UniGeneiMm.370270.

Genome annotation databases

EnsembliENSMUST00000074989; ENSMUSP00000074516; ENSMUSG00000063382. [Q67FY2-1]
GeneIDi80288.
KEGGimmu:80288.
UCSCiuc009pdu.1. mouse. [Q67FY2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128033 mRNA. Translation: BAD24964.1.
AY296058 mRNA. Translation: AAQ62696.1.
BC072555 mRNA. Translation: AAH72555.1.
BC082304 mRNA. Translation: AAH82304.1.
CCDSiCCDS40601.1. [Q67FY2-1]
RefSeqiNP_084532.2. NM_030256.2. [Q67FY2-1]
XP_006510764.1. XM_006510701.2. [Q67FY2-1]
XP_006510765.1. XM_006510702.2. [Q67FY2-1]
XP_006510766.1. XM_006510703.2. [Q67FY2-1]
XP_006510767.1. XM_006510704.2. [Q67FY2-1]
XP_006510768.1. XM_006510705.2. [Q67FY2-2]
UniGeneiMm.370270.

3D structure databases

ProteinModelPortaliQ67FY2.
SMRiQ67FY2. Positions 238-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219776. 1 interaction.
IntActiQ67FY2. 5 interactions.
STRINGi10090.ENSMUSP00000074516.

PTM databases

iPTMnetiQ67FY2.
PhosphoSiteiQ67FY2.

Proteomic databases

EPDiQ67FY2.
MaxQBiQ67FY2.
PaxDbiQ67FY2.
PRIDEiQ67FY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074989; ENSMUSP00000074516; ENSMUSG00000063382. [Q67FY2-1]
GeneIDi80288.
KEGGimmu:80288.
UCSCiuc009pdu.1. mouse. [Q67FY2-1]

Organism-specific databases

CTDi283149.
MGIiMGI:1933114. Bcl9l.

Phylogenomic databases

eggNOGiENOG410IEDB. Eukaryota.
ENOG410XRMX. LUCA.
GeneTreeiENSGT00730000110915.
HOGENOMiHOG000060118.
HOVERGENiHBG031116.
InParanoidiQ67FY2.
OMAiHQLMEKR.
OrthoDBiEOG7327N1.
PhylomeDBiQ67FY2.
TreeFamiTF331144.

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.

Miscellaneous databases

ChiTaRSiBcl9l. mouse.
NextBioi349989.
PROiQ67FY2.
SOURCEiSearch...

Gene expression databases

BgeeiQ67FY2.
CleanExiMM_BCL9L.
GenevisibleiQ67FY2. MM.

Family and domain databases

InterProiIPR015668. Bcl-9.
IPR024670. BCL9_beta-catenin-bd_dom.
[Graphical view]
PANTHERiPTHR15185. PTHR15185. 1 hit.
PfamiPF11502. BCL9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Role of a BCL9-related beta-catenin-binding protein, B9L, in tumorigenesis induced by aberrant activation of Wnt signaling."
    Adachi S., Jigami T., Yasui T., Nakano T., Ohwada S., Omori Y., Sugano S., Ohkawara B., Shibuya H., Nakamura T., Akiyama T.
    Cancer Res. 64:8496-8501(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CTNNB1, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions."
    Brembeck F.H., Schwarz-Romond T., Bakkers J., Wilhelm S., Hammerschmidt M., Birchmeier W.
    Genes Dev. 18:2225-2230(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH CTNNB1.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1091-1494 (ISOFORM 1/2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Crystal structure of a beta-catenin/BCL9/Tcf4 complex."
    Sampietro J., Dahlberg C.L., Cho U.S., Hinds T.R., Kimelman D., Xu W.
    Mol. Cell 24:293-300(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CTNNB1.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-994; SER-1001 AND SER-1007, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-912; SER-923; SER-939; SER-944; SER-984; SER-988; SER-994; SER-1001 AND SER-1007, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver, Lung, Spleen and Testis.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-36; LYS-108; LYS-110 AND LYS-137, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiBCL9L_MOUSE
AccessioniPrimary (citable) accession number: Q67FY2
Secondary accession number(s): Q641L9, Q6GQY0, Q6I7B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.