Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Gentisate 1,2-dioxygenase

Gene
N/A
Organism
Pseudaminobacter salicylatoxidans
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Iron; via tele nitrogenCombined sources
Metal bindingi121 – 1211Iron; via tele nitrogenCombined sources
Metal bindingi160 – 1601Iron; via tele nitrogenCombined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

DioxygenaseImported, Oxidoreductase

Keywords - Ligandi

IronCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15927.

Names & Taxonomyi

Protein namesi
Submitted name:
Gentisate 1,2-dioxygenaseImported
OrganismiPseudaminobacter salicylatoxidansImported
Taxonomic identifieri93369 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaePseudaminobacter

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PHDX-ray2.90A/B/C/D1-368[»]
3NJZX-ray2.10A1-368[»]
3NKTX-ray2.35A1-368[»]
3NL1X-ray2.15A1-368[»]
3NSTX-ray2.40A1-368[»]
3NVCX-ray2.45A1-368[»]
3NW4X-ray2.00A1-368[»]
4FAGX-ray2.50A1-367[»]
4FAHX-ray2.50A1-367[»]
4FBFX-ray2.70A1-367[»]
ProteinModelPortaliQ67FT0.
SMRiQ67FT0. Positions 15-366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ67FT0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 17468Cupin_2InterPro annotationAdd
BLAST
Domaini295 – 34147Cupin_2InterPro annotationAdd
BLAST

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR013096. Cupin_2.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67FT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNEKLDHES VTQAMQPKDT PELRALYKSF EEESIIPLWT QLGDLMPIHP
60 70 80 90 100
KSKAVPHVWK WSTLLRLARK SGELVPVGRG GERRALGLAN PGLGGNAYIS
110 120 130 140 150
PTMWAGIQYL GPRETAPEHR HSQNAFRFVV EGEGVWTVVN GDPVRMSRGD
160 170 180 190 200
LLLTPGWCFH GHHNDTDQPM AWIDGLDIPF SQQMDVGFFE FGSDRVTDYA
210 220 230 240 250
TPNFSRGERL WCHPGLRPLS GLQNTVASPI GAYRWEFTDR ALTEQLLLED
260 270 280 290 300
EGQPATVAPG HAAIRYVNPT TGGDVMPTLR CEFHRLRAGT ETATRNEVGS
310 320 330 340 350
TVFQVFEGAG AVVMNGETTK LEKGDMFVVP SWVPWSLQAE TQFDLFRFSD
360
APIMEALSFM RTKIEGQK
Length:368
Mass (Da):41,106
Last modified:October 11, 2004 - v1
Checksum:i92954E1BC60D040E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323951 Genomic DNA. Translation: AAQ91293.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323951 Genomic DNA. Translation: AAQ91293.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PHDX-ray2.90A/B/C/D1-368[»]
3NJZX-ray2.10A1-368[»]
3NKTX-ray2.35A1-368[»]
3NL1X-ray2.15A1-368[»]
3NSTX-ray2.40A1-368[»]
3NVCX-ray2.45A1-368[»]
3NW4X-ray2.00A1-368[»]
4FAGX-ray2.50A1-367[»]
4FAHX-ray2.50A1-367[»]
4FBFX-ray2.70A1-367[»]
ProteinModelPortaliQ67FT0.
SMRiQ67FT0. Positions 15-366.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15927.

Miscellaneous databases

EvolutionaryTraceiQ67FT0.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR013096. Cupin_2.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Biochemical and molecular characterization of a ring fission dioxygenase with the ability to oxidize (substituted) salicylate(s) from Pseudaminobacter salicylatoxidans."
    Hintner J.P., Reemtsma T., Stolz A.
    J. Biol. Chem. 279:37250-37260(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Crystal Structure Determination of a Salicylate 1,2-Dioxygenase from Pseudaminobacter salicylatoxidans."
    Ferraroni M., Matera I., Briganti F., Stolz A., Burger S.
    Submitted (APR-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) IN COMPLEX WITH IRON.
  3. "Crystal Structures of Salicylate 1,2-dioxygenase G106A mutant from Pseudoaminobacter salicylatoxidans and its adducts with substrates."
    Matera I., Ferraroni M., Briganti F., Stolz A., Steimer L., Burger S.
    Submitted (JUL-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH IRON, X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH IRON, X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH IRON.
  4. "Crystal structures of salicylate 1,2-dioxygenase-substrates adducts: A step towards the comprehension of the structural basis for substrate selection in class III ring cleaving dioxygenases."
    Ferraroni M., Matera I., Steimer L., Burger S., Scozzafava A., Stolz A., Briganti F.
    J. Struct. Biol. 177:431-438(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH IRON.
  5. "The generation of a 1-hydroxy-2-naphthoate 1,2-dioxygenase by single point mutations of salicylate 1,2-dioxygenase--rational design of mutants and the crystal structures of the A85H and W104Y variants."
    Ferraroni M., Steimer L., Matera I., Burger S., Scozzafava A., Stolz A., Briganti F.
    J. Struct. Biol. 180:563-571(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1-367 IN COMPLEX WITH IRON.

Entry informationi

Entry nameiQ67FT0_PSESE
AccessioniPrimary (citable) accession number: Q67FT0
Entry historyi
Integrated into UniProtKB/TrEMBL: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.