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Protein

Bactericidal permeability-increasing protein

Gene

Bpi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Bactericidal permeability-increasing protein
Short name:
BPI
Gene namesi
Name:Bpi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3045315. Bpi.

Subcellular locationi

  • Secreted By similarity
  • Cytoplasmic granule membrane 1 Publication

  • Note: Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.1 Publication

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: InterPro
  • membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 483456Bactericidal permeability-increasing proteinPRO_0000358332Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi161 ↔ 201By similarity
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ67E05.
PRIDEiQ67E05.

PTM databases

iPTMnetiQ67E05.
PhosphoSiteiQ67E05.

Expressioni

Tissue specificityi

Expressed in testis, epididymis, and bone marrow, as well as in Sertoli and promyelocytic cell lines. Upon stimulation with different TLR ligands, it is strongly expressed in granulocytes and in bone marrow-derived dendritic cells.2 Publications

Inductioni

By lipopolysaccharide (LPS) through TRL4-TRIF-dependent pathway. Expression in Sertoli and promyelocytic cells is enhanced several-fold by all-trans retinoic acid.2 Publications

Gene expression databases

BgeeiQ67E05.

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067837.

Structurei

3D structure databases

ProteinModelPortaliQ67E05.
SMRiQ67E05. Positions 29-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 188183N-terminal barrel By similarityBy similarityAdd
BLAST
Regioni189 – 25466Central sheet By similarityBy similarityAdd
BLAST
Regioni235 – 2406Cleavage sites for elastaseBy similarity
Regioni255 – 426172C-terminal barrel By similarityBy similarityAdd
BLAST

Domaini

The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested (By similarity).By similarity
The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4160. Eukaryota.
ENOG410Z88E. LUCA.
GeneTreeiENSGT00730000110583.
HOGENOMiHOG000231250.
HOVERGENiHBG002797.
InParanoidiQ67E05.
OMAiQLYRKYP.
OrthoDBiEOG76739B.
TreeFamiTF315617.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR030181. BPI.
IPR030675. BPI/LBP.
IPR032942. BPI/LBP/Plunc.
IPR001124. Lipid-bd_serum_glycop_C.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 1 hit.
PTHR10504:SF84. PTHR10504:SF84. 1 hit.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
PF02886. LBP_BPI_CETP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002417. Lipid_binding_protein. 1 hit.
SMARTiSM00328. BPI1. 1 hit.
SM00329. BPI2. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q67E05-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTWAPDNVRK WSALLLLAII GTALTAATDP GFVAMISQKG LDFACQQGVV
60 70 80 90 100
ELQKELQAIS VPDFSGVFKI KHLGKGSYEF YSMAVDGFHI PNPKIEMLPS
110 120 130 140 150
DGLRVFIKDA SIKINGKWMS RKNFLKAGGN FELSIQGVSI STDLILGSDS
160 170 180 190 200
SGHITTICSN CDSHIDSVHI KISGSMLGWL IRLFHRKIET SLKNIIYKKI
210 220 230 240 250
CKIVRDSVSS KLQPYLKTLS VITRVDDVTS VDYSLLAPLT TTNQFLEGQL
260 270 280 290 300
KGEFFWRGHR DPLPIHPPVM RFVPNGAYMV CMGISDYFFN TEVLAYQQSG
310 320 330 340 350
TLKMTLGGQL LSNNGRFQLN TDFLRTFLPK VAKMFPSMGV QLLISAPVPV
360 370 380 390 400
HLSIQPSGLS FNPKLETQAF VVLPNASLVP LFVLGMKTNA SLEVDAEENR
410 420 430 440 450
LVGEMKLGSR WLLELKESKF GPFKVEYLED VINYLVSTLV LPKINERLRR
460 470 480
GFPLPLPAGI RFSHFTFYPY QNFLLLEADL HLI
Length:483
Mass (Da):53,940
Last modified:October 11, 2004 - v1
Checksum:i4AA2D48095C52B74
GO
Isoform 2 (identifier: Q67E05-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     409-409: Missing.

Show »
Length:482
Mass (Da):53,853
Checksum:i5AA75217D0F72260
GO
Isoform 3 (identifier: Q67E05-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     386-386: M → MVRR

Show »
Length:486
Mass (Da):54,352
Checksum:i9D8F627EA5496D62
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei386 – 3861M → MVRR in isoform 3. 1 PublicationVSP_036069
Alternative sequencei409 – 4091Missing in isoform 2. CuratedVSP_036070

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY853179 mRNA. Translation: AAW50819.1.
AY648037 mRNA. Translation: AAV65841.1.
AY363993 mRNA. Translation: AAR13289.1.
AK033770 mRNA. Translation: BAC28468.1.
AL663063 Genomic DNA. Translation: CAM23524.1.
AL663063 Genomic DNA. Translation: CAM23525.1.
AL663063 Genomic DNA. Translation: CAM23526.1.
BC118049 mRNA. Translation: AAI18050.1.
BC118504 mRNA. Translation: AAI18505.1.
CCDSiCCDS16987.1. [Q67E05-3]
RefSeqiNP_808518.1. NM_177850.3. [Q67E05-3]
UniGeneiMm.260883.

Genome annotation databases

EnsembliENSMUST00000065039; ENSMUSP00000067837; ENSMUSG00000052922. [Q67E05-3]
ENSMUST00000109499; ENSMUSP00000105125; ENSMUSG00000052922. [Q67E05-1]
ENSMUST00000109500; ENSMUSP00000105126; ENSMUSG00000052922. [Q67E05-2]
GeneIDi329547.
KEGGimmu:329547.
UCSCiuc008npw.1. mouse. [Q67E05-3]
uc008npx.1. mouse. [Q67E05-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY853179 mRNA. Translation: AAW50819.1.
AY648037 mRNA. Translation: AAV65841.1.
AY363993 mRNA. Translation: AAR13289.1.
AK033770 mRNA. Translation: BAC28468.1.
AL663063 Genomic DNA. Translation: CAM23524.1.
AL663063 Genomic DNA. Translation: CAM23525.1.
AL663063 Genomic DNA. Translation: CAM23526.1.
BC118049 mRNA. Translation: AAI18050.1.
BC118504 mRNA. Translation: AAI18505.1.
CCDSiCCDS16987.1. [Q67E05-3]
RefSeqiNP_808518.1. NM_177850.3. [Q67E05-3]
UniGeneiMm.260883.

3D structure databases

ProteinModelPortaliQ67E05.
SMRiQ67E05. Positions 29-480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067837.

PTM databases

iPTMnetiQ67E05.
PhosphoSiteiQ67E05.

Proteomic databases

PaxDbiQ67E05.
PRIDEiQ67E05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065039; ENSMUSP00000067837; ENSMUSG00000052922. [Q67E05-3]
ENSMUST00000109499; ENSMUSP00000105125; ENSMUSG00000052922. [Q67E05-1]
ENSMUST00000109500; ENSMUSP00000105126; ENSMUSG00000052922. [Q67E05-2]
GeneIDi329547.
KEGGimmu:329547.
UCSCiuc008npw.1. mouse. [Q67E05-3]
uc008npx.1. mouse. [Q67E05-1]

Organism-specific databases

CTDi671.
MGIiMGI:3045315. Bpi.

Phylogenomic databases

eggNOGiKOG4160. Eukaryota.
ENOG410Z88E. LUCA.
GeneTreeiENSGT00730000110583.
HOGENOMiHOG000231250.
HOVERGENiHBG002797.
InParanoidiQ67E05.
OMAiQLYRKYP.
OrthoDBiEOG76739B.
TreeFamiTF315617.

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.

Miscellaneous databases

PROiQ67E05.
SOURCEiSearch...

Gene expression databases

BgeeiQ67E05.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR030181. BPI.
IPR030675. BPI/LBP.
IPR032942. BPI/LBP/Plunc.
IPR001124. Lipid-bd_serum_glycop_C.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 1 hit.
PTHR10504:SF84. PTHR10504:SF84. 1 hit.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
PF02886. LBP_BPI_CETP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002417. Lipid_binding_protein. 1 hit.
SMARTiSM00328. BPI1. 1 hit.
SM00329. BPI2. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A murine antibacterial ortholog to human bactericidal/permeability-increasing protein (BPI) is expressed in testis, epididymis, and bone marrow."
    Lennartsson A., Pieters K., Vidovic K., Gullberg U.
    J. Leukoc. Biol. 77:369-377(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Testis.
  2. "Endotoxin-induced expression of murine bactericidal permeability/increasing protein is mediated exclusively by toll/IL-1 receptor domain-containing adaptor inducing IFN-beta-dependent pathways."
    Eckert M., Wittmann I., Roellinghoff M., Gessner A., Schnare M.
    J. Immunol. 176:522-528(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  3. Bingle C.D., Craven J.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Swiss Webster.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Epididymis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiBPI_MOUSE
AccessioniPrimary (citable) accession number: Q67E05
Secondary accession number(s): A2AC63, Q5I5I4, Q8BSF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.