Reviewed, UniProtKB/Swiss-Prot Q67E01 (DUSTY_XENLA)

Names and origin

Protein names

Recommended name:
    Dual serine/threonine and tyrosine protein kinase
    EC=2.7.12.1
Alternative name(s):
    Dusty protein kinase
      Short name=Dusty PK
    Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	dstyk
Synonyms:	ripk5

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May induce both caspase-dependent apoptosis and caspase-independent cell death By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 916	916	Dual serine/threonine and tyrosine protein kinase		PRO_0000233125
Regions
	Domain	641 – 895	255	Protein kinase
	Nucleotide binding	647 – 655	9	ATP By similarity
Sites
	Active site	766	1	Proton acceptor By similarity
	Binding site	670	1	ATP By similarity
Experimental info
	Sequence conflict	605	1	E → K in AAH97571. Ref.2
	Sequence conflict	739	1	S → T in AAH97571. Ref.2
	Sequence conflict	762	1	V → L in AAH97571. Ref.2
	Sequence conflict	907 – 916	10	ETGKALEDST → KKRKKKKKKKKKK in AAH97571. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q67E01-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5B622AF8C5D57A40	FASTA	916	104,164
	10 20 30 40 50 60 MQRDGTRSAR RMDEGDRRTG SAGRSGSREL GRGFSYYNKY LARLQQNLRD TKRFFRDIKL 70 80 90 100 110 120 TYSGAPGGPD TEFSGAEGEF GQLHSITFPR QEEEYLKLTV RCRPCIFILG QNCSGRGRVA 130 140 150 160 170 180 NGLLGGQLLP ILTHGDIECC KRRRIRFRHG KQTLVSLALP EQYELVHQLV AHQGKWDTIP 190 200 210 220 230 240 EEDLDVPEDE EDPAHRLAEL EVTLPHQLLQ DVDIVVSPCR SSQAVSMTLE DYVDHVQSIV 250 260 270 280 290 300 VYAVSEEMLS KQDEEELTEI KEKYKLPVFF IRTSSTKDRL IGGSEGVRSV LYEQLIELGY 310 320 330 340 350 360 LQRGLCNCGA AGSGSTAPSM LVEQFEKIRQ LSTFSRQVLQ MHLVDAAIVL NMVHSRCLDL 370 380 390 400 410 420 FINKAFDMHR DLQITPKRLE YTRQKENELY ESLMRISDRK QEELKDMIVE TLNSMREQLL 430 440 450 460 470 480 EDAANMQFKD IVIPQNGEPV SAHEVKCCIF QIKELIISRL NQAVVNKLIS SVDYLRESFV 490 500 510 520 530 540 GTMERCLRSL EKSQQESSSH VTSNHLKQIL NAAYHVEVTF NSGSTVTRMV WEQIVQIIQR 550 560 570 580 590 600 ITWVSPPTIT PEWKRRVAQD AIETLSASKL AKSICSQFCK RLKSSHEAFA ASLKQLEVGH 610 620 630 640 650 660 SGRLEKTNDL WLRVRKDHAP RLARLSLESR SLQDVLLHGK PRIGRELGRG QYGVVYLCDS 670 680 690 700 710 720 WGGHFPCALK SVVPPDEKHW NDLALEFHYM RSLPKHERLV DLHGSVIDYS YGGGSSIAVL 730 740 750 760 770 780 LITERLHRDL YVGLKTGLSL ETRLQIALDV VEGIRFLHNQ GVVHRDIKLK NVLLDKKHRA 790 800 810 820 830 840 KITDLGFCKP EAMMSGSIVG TPIHMAPELF SGKYDNSVDV YAFGILFWYI CSGSVKLPEA 850 860 870 880 890 900 FEKCASKDHL WNNVRKGARP ERLTIFDEEC WKLMEACWNG DPSQRPLMGI VQPMLQGIMD 910 RLCHTQETGK ALEDST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY364232 mRNA. Translation: AAR13047.1. AY429678 mRNA. Translation: AAS55394.1. BC097571 mRNA. Translation: AAH97571.1.
RefSeq	NP_001087088.1.
UniGene	Xl.5447
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	446954.
KEGG	xla:446954.
Organism-specific databases
CTD	446954.
Xenbase	XB-FEAT-971396. dstyk.
Phylogenomic databases
HOVERGEN	Q67E01.
Enzyme and pathway databases
BRENDA	2.7.11.1. 648.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSTY_XENLA

Accession

Primary (citable) accession number: Q67E01
Secondary accession number(s): Q4QR51

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	October 11, 2004
Last modified:	September 1, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project