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Protein

Lactosylceramide 4-alpha-galactosyltransferase

Gene

A4galt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins (By similarity).By similarity

Catalytic activityi

UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide = UDP + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • galactosyltransferase activity Source: MGI
  • lactosylceramide 4-alpha-galactosyltransferase activity Source: MGI
  • toxic substance binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide 4-alpha-galactosyltransferase (EC:2.4.1.228)
Alternative name(s):
Alpha-1,4-N-acetylglucosaminyltransferase
Alpha-1,4-galactosyltransferase
Alpha4Gal-T1
Globotriaosylceramide synthase
Short name:
Gb3 synthase
UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
Gene namesi
Name:A4galtImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:3512453. A4galt.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicSequence analysisAdd
BLAST
Transmembranei31 – 5121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini52 – 359308LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Lactosylceramide 4-alpha-galactosyltransferasePRO_0000080579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ67BJ4.
PRIDEiQ67BJ4.

PTM databases

iPTMnetiQ67BJ4.
PhosphoSiteiQ67BJ4.

Expressioni

Gene expression databases

BgeeiQ67BJ4.
CleanExiMM_A4GALT.
ExpressionAtlasiQ67BJ4. baseline and differential.
GenevisibleiQ67BJ4. MM.

Interactioni

Protein-protein interaction databases

MINTiMINT-4997421.
STRINGi10090.ENSMUSP00000057999.

Structurei

3D structure databases

ProteinModelPortaliQ67BJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi198 – 2003DXD motifBy similarity

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG050427.
InParanoidiQ67BJ4.
KOiK01988.
OMAiFVAHYNG.
OrthoDBiEOG75MVW8.
PhylomeDBiQ67BJ4.
TreeFamiTF324053.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67BJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGISCSHLEE TMSKPPDCLL RMLRGTPRQR VFTFFIISFK FMFLISILIY
60 70 80 90 100
WHTVGAPKDQ REYSLPVDFS CPQLAFPRVS APGNIFFLET SDRTSPNFLF
110 120 130 140 150
MCSVESAARA HPESQVVVLM KGLPRDTTAQ PRNLGISLLS CFPNVWIRPL
160 170 180 190 200
DLQELFEDTP LAAWYSEARH RWEPYQLPVL SDASRIALLW KFGGIYLDTD
210 220 230 240 250
FIVLKNLLNL TNTLGIQSRY VLNGAFLAFE RKHEFLALCL HDFVANYNGW
260 270 280 290 300
IWGHQGPQLL TRVFKKWCSI QSLEKSHACR GVTALPPEAF YPIPWQNWKK
310 320 330 340 350
YFEDISPEEL TQLLNATYAV HVWNKKSQGT HLEATSKALL AQLHARYCPT

THRAMKMYL
Length:359
Mass (Da):41,366
Last modified:October 11, 2004 - v1
Checksum:iB6380E88BF0A4676
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY371179 mRNA. Translation: AAR18365.1.
CCDSiCCDS27699.1.
RefSeqiNP_001004150.1. NM_001004150.3.
NP_001164425.1. NM_001170954.1.
UniGeneiMm.323805.

Genome annotation databases

EnsembliENSMUST00000049530; ENSMUSP00000057999; ENSMUSG00000047878.
ENSMUST00000164614; ENSMUSP00000129719; ENSMUSG00000047878.
GeneIDi239559.
KEGGimmu:239559.
UCSCiuc007xae.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

a4GalT

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY371179 mRNA. Translation: AAR18365.1.
CCDSiCCDS27699.1.
RefSeqiNP_001004150.1. NM_001004150.3.
NP_001164425.1. NM_001170954.1.
UniGeneiMm.323805.

3D structure databases

ProteinModelPortaliQ67BJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997421.
STRINGi10090.ENSMUSP00000057999.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

PTM databases

iPTMnetiQ67BJ4.
PhosphoSiteiQ67BJ4.

Proteomic databases

PaxDbiQ67BJ4.
PRIDEiQ67BJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049530; ENSMUSP00000057999; ENSMUSG00000047878.
ENSMUST00000164614; ENSMUSP00000129719; ENSMUSG00000047878.
GeneIDi239559.
KEGGimmu:239559.
UCSCiuc007xae.2. mouse.

Organism-specific databases

CTDi53947.
MGIiMGI:3512453. A4galt.

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG050427.
InParanoidiQ67BJ4.
KOiK01988.
OMAiFVAHYNG.
OrthoDBiEOG75MVW8.
PhylomeDBiQ67BJ4.
TreeFamiTF324053.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ67BJ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ67BJ4.
CleanExiMM_A4GALT.
ExpressionAtlasiQ67BJ4. baseline and differential.
GenevisibleiQ67BJ4. MM.

Family and domain databases

InterProiIPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Mouse Gb3 synthase."
    Li Y., Abe A., Hiraoka M., Shayman J.A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiA4GAT_MOUSE
AccessioniPrimary (citable) accession number: Q67BJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.