Q67726 (NS1AB_HASV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-structural polyprotein 1AB

Cleaved into the following 5 chains:

1. Protein p19
2. Transmembrane protein 1A
3. Serine protease p27
   Short name=p27
   EC=3.4.21.-
4. Protein p20
5. RNA-directed RNA polymerase p57
   Short name=p57
   EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Human astrovirus-1 (HAstV-1)

Taxonomic identifier

12456 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host membrane Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q67726-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 919.

Isoform nsp1a (identifier: P0C6K4-1)

The sequence of this isoform can be found in the external entry P0C6K4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1436	1436	Non-structural polyprotein 1AB		PRO_0000327271
	Chain	1 – 175	175	Protein p19 Potential		PRO_0000327272
	Chain	176 – 419	244	Transmembrane protein 1A Potential		PRO_0000327273
	Chain	420 – 664	245	Serine protease p27 Potential		PRO_0000327274
	Chain	665 – 933	269	Protein p20 Potential		PRO_0000327275
	Chain	934 – 1436	503	RNA-directed RNA polymerase p57 Potential		PRO_0000327276
Regions
	Transmembrane	156 – 176	21	Helical; Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Transmembrane	286 – 306	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	344 – 364	21	Helical; Potential
	Domain	1181 – 1307	127	RdRp catalytic
	Coiled coil	104 – 142	39	Potential
	Coiled coil	587 – 620	34	Potential
Sites
	Active site	461	1	Charge relay system; for serine protease activity By similarity
	Active site	489	1	Charge relay system; for serine protease activity By similarity
	Active site	551	1	Charge relay system; for serine protease activity By similarity
	Site	175 – 176	2	Cleavage Potential
	Site	419 – 420	2	Cleavage Potential
	Site	664 – 665	2	Cleavage Potential
	Site	933 – 934	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CEC41D44BCACD32F	FASTA	1,436	163,874
	10 20 30 40 50 60 MAYGEPYYSS KPDKDFNFGS TMARRQMTPT MVTKLPKFVR NSPQAYDWIV RGLIFPTIGK 70 80 90 100 110 120 TYFQRVVVIT GGLEDGTYGS FAFDGKEWVG IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ 130 140 150 160 170 180 EEKATLALDV QFLQHENVRL KEMIPKPEPR KIQMKWIIMG AVLTFLSLIP GGYAHSQTNN 190 200 210 220 230 240 TIFTDMIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRAHWLRTVF 250 260 270 280 290 300 YYIHYYEMWN IFMFVLAIGT VMRSARPGTD LVTLATSHLS GFRMAVLPTI PFHTTMTLWV 310 320 330 340 350 360 MNTLMVCYYF DNLLAITLAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT 370 380 390 400 410 420 LTGTTTSLFV VILTCRFIRM ATVFIGTRFE IRDANGKVVA TVPTRIKNVA FDFFQKLKQS 430 440 450 460 470 480 GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HVVGNNTFVN VCYEGLMYEA 490 500 510 520 530 540 KVRYMPEKDI AFLTCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL 550 560 570 580 590 600 SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PADFHPVKAP SQVELLKEEI 610 620 630 640 650 660 ERLKAQLNSA TENATTVVTQ QPSAALEQKS VSDSDVVDLV RTAMEREMKV LRDEINGILA 670 680 690 700 710 720 PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY 730 740 750 760 770 780 PDYDDEDYYD EDDDGWGMVG DDVEFDYTEV INFDQAKPIP APRTTKQKIC PEPEVESQPL 790 800 810 820 830 840 DLSQKKEKQS EYEQQVVKST KPQQLEHEQQ VVKPIKPQKS EPQPYSQTYG KAPIWESYDF 850 860 870 880 890 900 DWDEDDAKFI LPAPHRLTKA DEIVLGSKIV KLRTIIETAI KTQNYSALPE AVFELDKAAY 910 920 930 940 950 960 EAGLEGFLQR VKSKNKAPEK QGPKKLQRAP EDQGAQNYHS LDAWKLLLEP PRERRCVPAN 970 980 990 1000 1010 1020 FPLLGHLPIN RPIFDDKKPR DDLLGLLPEP TWHAFEEYGP TTWGPQAFIK SFDKFFYAEP 1030 1040 1050 1060 1070 1080 IDFFSEYPQL CAFADWATYR EFRYLEDTRV IHITATEKNT DSTPAYPKMN YFDTEENYLE 1090 1100 1110 1120 1130 1140 AHGWAPYIRE FTRVYKGDKP EVLWYLFLKK EIIKEEKIRN SDIRQIVCAD PIYTRIGACL 1150 1160 1170 1180 1190 1200 EAHQNALMKQ HTDTSVGQCG WSPMEGGFKK TMQRLVNKGN KHFIEFDWTR YDGTIPPALF 1210 1220 1230 1240 1250 1260 KHIKEIRWNF INKDQREKYR HVHEWYVNNL LNRHVLLPSG EVTLQTRGNP SGQFSTTMDN 1270 1280 1290 1300 1310 1320 NMVNFWLQAF EFAYFNGPDR DLWKTYDTVV YGDDRLSTTP SVPDDYEERV ITMYRDIFGM 1330 1340 1350 1360 1370 1380 WVKPGKVICR DSIVGLSFCG FTVNENLEPV PTSPEKLMAS LLKPYKILPD LESLHGKLLC 1390 1400 1410 1420 1430 YQLLAAFMAE DHPFKVYVEH CLSRTAKQLR DSGLPARLTE EQLHRIWRGG PKKCDG
	Isoform nsp1a [UniParc]. See P0C6K4.		–	–

References

[1]	"The complete sequence of a human astrovirus." Willcocks M.M., Brown T.D., Madeley C.R., Carter M.J. J. Gen. Virol. 75:1785-1788(1994) [PubMed: 8021608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein." Lewis T.L., Greenberg H.B., Herrmann J.E., Smith L.S., Matsui S.M. J. Virol. 68:77-83(1994) [PubMed: 8254779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Processing of nonstructural protein 1a of human astrovirus." Geigenmuller U., Chew T., Ginzton N., Matsui S.M. J. Virol. 76:2003-2008(2002) [PubMed: 11799197] [Abstract] Cited for: NSP1A POLYPROTEIN PROCESSING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25771 Genomic RNA. Translation: CAA81033.1.
PIR	C49529.
3D structure databases
ProteinModelPortal	Q67726.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022068. Astroviridae_polyprotein_1. IPR009003. Pept_cys/ser_Trypsin-like. IPR001205. RNA-dir_pol_picornavirus. IPR007094. RNA-dir_pol_PSvirus. [Graphical view]
Pfam	PF12285. DUF3621. 2 hits. PF00680. RdRP_1. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_HASV1

Accession

Primary (citable) accession number: Q67726

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program