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Protein

Replication-associated protein

Gene

C1/C2

Organism
Wheat dwarf virus (isolate Sweden) (WDV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. Acts as an inhibitor of C-sense gene transcription (By similarity).By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations, possibly Mg2+ or Mn2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Divalent metal cationSequence analysis
Metal bindingi59 – 591Divalent metal cationSequence analysis
Metal bindingi61 – 611Divalent metal cationSequence analysis
Active sitei106 – 1061For DNA cleavage activityBy similarity
Metal bindingi110 – 1101Divalent metal cationSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2308ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • rolling circle single-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Repressor, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-)
Short name:
Rep
Gene namesi
ORF Names:C1/C2
OrganismiWheat dwarf virus (isolate Sweden) (WDV)
Taxonomic identifieri268789 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiAvena sativa (Oat) [TaxID: 4498]
Hordeum vulgare (Barley) [TaxID: 4513]
Lolium multiflorum (Italian ryegrass) (Lolium perenne subsp. multiflorum) [TaxID: 4521]
Secale cereale (Rye) [TaxID: 4550]
Triticum aestivum (Wheat) [TaxID: 4565]
Proteomesi
  • UP000007629 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Replication-associated proteinPRO_0000316945Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Homooligomer. Rep binds to repeated DNA motifs (iterons). Forms the O-complex, which is a Rep-DNA complex involved in the initiation of RCR. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ67622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 18513OligomerizationBy similarityAdd
BLAST
Regioni246 – 26419TransactivationBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi17 – 215RCR-1
Motifi59 – 646RCR-2
Motifi106 – 1094RCR-3
Motifi286 – 29611Nuclear localization signalSequence analysisAdd
BLAST

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Rep (identifier: Q67622-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSAPRFR VYSKYLFLTY PECTLEPQYA LDSLRTLLNK YEPLYIAAVR
60 70 80 90 100
ELHEDGSPHL HVLVQNKLRA SITNPNALNL RMDTSPFSIF HPNIQAAKDC
110 120 130 140 150
NQVRDYITKE VDSDVNTAEW GTFVAVSTPG RKDRDADMKQ IIESSSSREE
160 170 180 190 200
FLSMVCNRFP FEWSIRLKDF EYTARHLFPD PVATYTPEFP TESLICHETI
210 220 230 240 250
ESWKNEHLYS ESPGRHKSIY ICGPTRTGKT SWARSLGTHN YYNSLVDFTT
260 270 280 290 300
YDVNAKYNII DDIPFKFTPN WKCFVGAQRD FTVNPKYGKR KVIRGGIPCI
310 320 330 340 350
ILVNPDEDWL KDMTPEQSDY MYSNTVVHYM YEGETFINYS FASGEDVTAS

Q
Length:351
Mass (Da):40,511
Last modified:February 5, 2008 - v2
Checksum:i51664B5AD96A2463
GO
Isoform RepA (identifier: P06847-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P06847.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced from the unspliced transcript.
Length:264
Mass (Da):30,157
GO

Sequence cautioni

The sequence CAA26624 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA26625 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02869 Genomic DNA. Translation: CAA26624.1. Sequence problems.
X02869 Genomic DNA. Translation: CAA26625.1. Sequence problems.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02869 Genomic DNA. Translation: CAA26624.1. Sequence problems.
X02869 Genomic DNA. Translation: CAA26625.1. Sequence problems.

3D structure databases

ProteinModelPortaliQ67622.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiREP_WDVS
AccessioniPrimary (citable) accession number: Q67622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: October 14, 2015
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.