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Protein

Transcriptional activator protein

Gene

C2

Organism
Tomato yellow leaf curl Sardinia virus (isolate Spain-2) (TYLCSV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Strong activator of the late viral genes promoters. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. TrAP suppresses the host RNA silencing by inhibiting adenosine kinase 2 (ADK2), a kinase involved in a general methylation pathway. Also suppresses the host basal defense by interacting with and inhibiting SNF1 kinase, a key regulator of cell metabolism implicated in innate antiviral defense. Determines pathogenicity (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 54By similarityAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Suppressor of RNA silencing

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein
Short name:
TrAP
Alternative name(s):
Protein C2
Protein L2
Gene namesi
ORF Names:C2, L2
OrganismiTomato yellow leaf curl Sardinia virus (isolate Spain-2) (TYLCSV)
Taxonomic identifieri221538 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeBegomovirus
Virus hostiCynanchum acutum [TaxID: 185024]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Solanum nigrum (Black nightshade) [TaxID: 4112]
Proteomesi
  • UP000008266 Componenti: Genome

Subcellular locationi

  • Host nucleus By similarity
  • Host cytoplasm By similarity

  • Note: The phosphorylated form appears to accumulate almost exclusively in the nucleus, whereas the non-phosphorylated form is found in both nucleus and cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003236811 – 135Transcriptional activator proteinAdd BLAST135

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer. Homodimer. Homooligomer. Self-interaction correlates with nuclear localization and efficient activation of transcription. Monomers suppress local silencing by interacting with and inactivating host adenosine kinase 2 (ADK2) in the cytoplasm. Interacts with and inhibits host SNF1 kinase. Binds to ssDNA (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 135TransactivationBy similarityAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi17 – 32Nuclear localization signalBy similarityAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 32Poly-Arg4

Domaini

The zinc finger and the transactivation region are involved in PTGS suppression.By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 54By similarityAdd BLAST18

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR000942. Gemini_AL2.
[Graphical view]
PfamiPF01440. Gemini_AL2. 1 hit.
[Graphical view]
PRINTSiPR00230. GEMCOATAL2.
ProDomiPD001117. Gemini_AL2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q67619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSSPSTSH CSQIPIKIQH HIAKKRQVRR RRVDLDCGCS YYIHLDCINH
60 70 80 90 100
GFTHRGVHHC ASSNEWRLYL RDNKSPIFHD NQTQPTTIQQ QIQFTNISNQ
110 120 130
VQPQLEEGTG DSQMFSQLPH LDDLTVSDWS FFKSL
Length:135
Mass (Da):15,691
Last modified:November 1, 1996 - v1
Checksum:i95E21245624EC9AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27708 Genomic DNA. Translation: AAA47954.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27708 Genomic DNA. Translation: AAA47954.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000942. Gemini_AL2.
[Graphical view]
PfamiPF01440. Gemini_AL2. 1 hit.
[Graphical view]
PRINTSiPR00230. GEMCOATAL2.
ProDomiPD001117. Gemini_AL2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAP_TYCS2
AccessioniPrimary (citable) accession number: Q67619
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.