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Q675L5

- TPSD2_PICAB

UniProt

Q675L5 - TPSD2_PICAB

Protein

Isopimaradiene synthase, chloroplastic

Gene

TPS-ISO

Organism
Picea abies (Norway spruce) (Picea excelsa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Involved in defensive oleoresin formation in conifers in response to insect attack or other injury By similarity. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to isopimara-7,15-diene.By similarity1 Publication

    Catalytic activityi

    Geranylgeranyl diphosphate = (+)-copalyl diphosphate.1 Publication
    +-copalyl diphosphate = isopimara-7,15-diene + diphosphate.1 Publication

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi619 – 6191Magnesium 1By similarity
    Metal bindingi619 – 6191Magnesium 2By similarity
    Metal bindingi623 – 6231Magnesium 1By similarity
    Metal bindingi623 – 6231Magnesium 2By similarity
    Metal bindingi763 – 7631Magnesium 3By similarity
    Metal bindingi767 – 7671Magnesium 3By similarity
    Metal bindingi771 – 7711Magnesium 3By similarity

    GO - Molecular functioni

    1. copalyl diphosphate synthase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. terpene synthase activity Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12744.
    BRENDAi4.2.3.35. 4815.
    UniPathwayiUPA00924.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isopimaradiene synthase, chloroplastic
    Alternative name(s):
    Diterpene synthase
    PaTPS-Iso
    Including the following 2 domains:
    Isopimara-7,15-diene synthase (EC:4.2.3.44)
    Short name:
    Isopimaradiene synthase
    Copalyl diphosphate synthase (EC:5.5.1.12)
    Gene namesi
    Name:TPS-ISO
    OrganismiPicea abies (Norway spruce) (Picea excelsa)
    Taxonomic identifieri3329 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePicea

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6868ChloroplastSequence AnalysisAdd
    BLAST
    Chaini69 – 867799Isopimaradiene synthase, chloroplasticPRO_0000348953Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ675L5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi619 – 6235DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family. Tpsd subfamily.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q675L5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLSSSLSS QIPTGSHPLT HTQCIPHFST TINAGISAGK PRSFYLRWGK    50
    GSNKIIACVG EGTTSLPYQS AEKTDSLSAP TLVKREFPPG FWKDHVIDSL 100
    TSSHKVSAAE EKRMETLISE IKNIFRSMGY GETNPSAYDT AWVARIPAVD 150
    GSEHPEFPET LEWILQNQLK DGSWGEGFYF LAYDRILATL ACIITLTLWR 200
    TGETQIRKGI EFFKTQAGKI EDEADSHRPS GFEIVFPAML KEAKVLGLDL 250
    PYELPFIKQI IEKREAKLER LPTNILYALP TTLLYSLEGL QEIVDWEKII 300
    KLQSKDGSFL TSPASTAAVF MRTGNKKCLE FLNFVLKKFG NHVPCHYPLD 350
    LFERLWAVDT VERLGIDHHF KEEIKDALDY VYSHWDERGI GWARENPIPD 400
    IDDTAMGLRI LRLHGYNVSS DVLKTFRDEN GEFFCFLGQT QRGVTDMLNV 450
    NRCSHVAFPG ETIMQEAKLC TERYLRNALE DVGAFDKWAL KKNIRGEVEY 500
    ALKYPWHRSM PRLEARSYIE HYGPNDVWLG KTMYMMPYIS NLKYLELAKL 550
    DFNHVQSLHQ KELRDLRRWW KSSGLSELKF TRERVTEIYF SAASFIFEPE 600
    FATCRDVYTK ISIFTVILDD LYDAHGTLDN LELFSEGVKR WDLSLVDRMP 650
    QDMKICFTVL YNTVNEIAVE GRKRQGRDVL GYIRNVLEIL LAAHTKEAEW 700
    SAARYVPSFD EYIENASVSI SLGTLVLISV LFTGEILTDD VLSKIGRGSR 750
    FLQLMGLTGR LVNDTKTYEA ERGQGEVASA VQCYMKEHPE ISEEEALKHV 800
    YTVMENALDE LNREFVNNRD VPDSCRRLVF ETARIMQLFY MEGDGLTLSH 850
    EMEIKEHVKN CLFQPVA 867
    Length:867
    Mass (Da):99,203
    Last modified:November 22, 2005 - v2
    Checksum:i7E27A5BBCC6062D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY473620 mRNA. Translation: AAS47690.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY473620 mRNA. Translation: AAS47690.2 .

    3D structure databases

    ProteinModelPortali Q675L5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00924 .
    BioCyci MetaCyc:MONOMER-12744.
    BRENDAi 4.2.3.35. 4815.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 3 hits.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily."
      Martin D.M., Faeldt J., Bohlmann J.
      Plant Physiol. 135:1908-1927(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, GENE FAMILY, NOMENCLATURE.
    2. Martin D.M., Keeling C., Bohlmann J.
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiTPSD2_PICAB
    AccessioniPrimary (citable) accession number: Q675L5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 50 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3