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Protein

Bifunctional isopimaradiene synthase, chloroplastic

Gene

TPS-ISO

Organism
Picea abies (Norway spruce) (Picea excelsa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury (By similarity). Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to isopimara-7,15-diene.By similarity1 Publication

Catalytic activityi

Geranylgeranyl diphosphate = (+)-copalyl diphosphate.1 Publication
+-copalyl diphosphate = isopimara-7,15-diene + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: oleoresin biosynthesis

This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi619Magnesium 1By similarity1
Metal bindingi619Magnesium 2By similarity1
Metal bindingi623Magnesium 1By similarity1
Metal bindingi623Magnesium 2By similarity1
Metal bindingi763Magnesium 3By similarity1
Metal bindingi767Magnesium 3By similarity1
Metal bindingi771Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12744.
BRENDAi4.2.3.35. 4815.
4.2.3.44. 4815.
UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional isopimaradiene synthase, chloroplastic
Alternative name(s):
Diterpene synthase
PaTPS-Iso
Including the following 2 domains:
Isopimara-7,15-diene synthase (EC:4.2.3.44)
Short name:
Isopimaradiene synthase
Copalyl diphosphate synthase (EC:5.5.1.12)
Gene namesi
Name:TPS-ISO
OrganismiPicea abies (Norway spruce) (Picea excelsa)
Taxonomic identifieri3329 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePicea

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68ChloroplastSequence analysisAdd BLAST68
ChainiPRO_000034895369 – 867Bifunctional isopimaradiene synthase, chloroplasticAdd BLAST799

Structurei

3D structure databases

ProteinModelPortaliQ675L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi400 – 403DXDD motifCurated4
Motifi619 – 623DDXXD motifCurated5

Domaini

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity in the class II active site relevant for the cyclization of GGPP. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.Curated

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK14042.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q675L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLSSSLSS QIPTGSHPLT HTQCIPHFST TINAGISAGK PRSFYLRWGK
60 70 80 90 100
GSNKIIACVG EGTTSLPYQS AEKTDSLSAP TLVKREFPPG FWKDHVIDSL
110 120 130 140 150
TSSHKVSAAE EKRMETLISE IKNIFRSMGY GETNPSAYDT AWVARIPAVD
160 170 180 190 200
GSEHPEFPET LEWILQNQLK DGSWGEGFYF LAYDRILATL ACIITLTLWR
210 220 230 240 250
TGETQIRKGI EFFKTQAGKI EDEADSHRPS GFEIVFPAML KEAKVLGLDL
260 270 280 290 300
PYELPFIKQI IEKREAKLER LPTNILYALP TTLLYSLEGL QEIVDWEKII
310 320 330 340 350
KLQSKDGSFL TSPASTAAVF MRTGNKKCLE FLNFVLKKFG NHVPCHYPLD
360 370 380 390 400
LFERLWAVDT VERLGIDHHF KEEIKDALDY VYSHWDERGI GWARENPIPD
410 420 430 440 450
IDDTAMGLRI LRLHGYNVSS DVLKTFRDEN GEFFCFLGQT QRGVTDMLNV
460 470 480 490 500
NRCSHVAFPG ETIMQEAKLC TERYLRNALE DVGAFDKWAL KKNIRGEVEY
510 520 530 540 550
ALKYPWHRSM PRLEARSYIE HYGPNDVWLG KTMYMMPYIS NLKYLELAKL
560 570 580 590 600
DFNHVQSLHQ KELRDLRRWW KSSGLSELKF TRERVTEIYF SAASFIFEPE
610 620 630 640 650
FATCRDVYTK ISIFTVILDD LYDAHGTLDN LELFSEGVKR WDLSLVDRMP
660 670 680 690 700
QDMKICFTVL YNTVNEIAVE GRKRQGRDVL GYIRNVLEIL LAAHTKEAEW
710 720 730 740 750
SAARYVPSFD EYIENASVSI SLGTLVLISV LFTGEILTDD VLSKIGRGSR
760 770 780 790 800
FLQLMGLTGR LVNDTKTYEA ERGQGEVASA VQCYMKEHPE ISEEEALKHV
810 820 830 840 850
YTVMENALDE LNREFVNNRD VPDSCRRLVF ETARIMQLFY MEGDGLTLSH
860
EMEIKEHVKN CLFQPVA
Length:867
Mass (Da):99,203
Last modified:November 22, 2005 - v2
Checksum:i7E27A5BBCC6062D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY473620 mRNA. Translation: AAS47690.2.

Genome annotation databases

KEGGiag:AAS47690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY473620 mRNA. Translation: AAS47690.2.

3D structure databases

ProteinModelPortaliQ675L5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAS47690.

Phylogenomic databases

KOiK14042.

Enzyme and pathway databases

UniPathwayiUPA00924.
BioCyciMetaCyc:MONOMER-12744.
BRENDAi4.2.3.35. 4815.
4.2.3.44. 4815.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTPSD2_PICAB
AccessioniPrimary (citable) accession number: Q675L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.