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Q675L5

- TPSD2_PICAB

UniProt

Q675L5 - TPSD2_PICAB

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Protein

Isopimaradiene synthase, chloroplastic

Gene
TPS-ISO
Organism
Picea abies (Norway spruce) (Picea excelsa)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury By similarity. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to isopimara-7,15-diene.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate = (+)-copalyl diphosphate.1 Publication
+-copalyl diphosphate = isopimara-7,15-diene + diphosphate.1 Publication

Cofactori

Binds 3 magnesium ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi619 – 6191Magnesium 1 By similarity
Metal bindingi619 – 6191Magnesium 2 By similarity
Metal bindingi623 – 6231Magnesium 1 By similarity
Metal bindingi623 – 6231Magnesium 2 By similarity
Metal bindingi763 – 7631Magnesium 3 By similarity
Metal bindingi767 – 7671Magnesium 3 By similarity
Metal bindingi771 – 7711Magnesium 3 By similarity

GO - Molecular functioni

  1. copalyl diphosphate synthase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. terpene synthase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12744.
BRENDAi4.2.3.35. 4815.
UniPathwayiUPA00924.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopimaradiene synthase, chloroplastic
Alternative name(s):
Diterpene synthase
PaTPS-Iso
Including the following 2 domains:
Isopimara-7,15-diene synthase (EC:4.2.3.44)
Short name:
Isopimaradiene synthase
Copalyl diphosphate synthase (EC:5.5.1.12)
Gene namesi
Name:TPS-ISO
OrganismiPicea abies (Norway spruce) (Picea excelsa)
Taxonomic identifieri3329 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePicea

Subcellular locationi

Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6868Chloroplast Reviewed predictionAdd
BLAST
Chaini69 – 867799Isopimaradiene synthase, chloroplasticPRO_0000348953Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ675L5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi619 – 6235DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q675L5-1 [UniParc]FASTAAdd to Basket

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MALLSSSLSS QIPTGSHPLT HTQCIPHFST TINAGISAGK PRSFYLRWGK    50
GSNKIIACVG EGTTSLPYQS AEKTDSLSAP TLVKREFPPG FWKDHVIDSL 100
TSSHKVSAAE EKRMETLISE IKNIFRSMGY GETNPSAYDT AWVARIPAVD 150
GSEHPEFPET LEWILQNQLK DGSWGEGFYF LAYDRILATL ACIITLTLWR 200
TGETQIRKGI EFFKTQAGKI EDEADSHRPS GFEIVFPAML KEAKVLGLDL 250
PYELPFIKQI IEKREAKLER LPTNILYALP TTLLYSLEGL QEIVDWEKII 300
KLQSKDGSFL TSPASTAAVF MRTGNKKCLE FLNFVLKKFG NHVPCHYPLD 350
LFERLWAVDT VERLGIDHHF KEEIKDALDY VYSHWDERGI GWARENPIPD 400
IDDTAMGLRI LRLHGYNVSS DVLKTFRDEN GEFFCFLGQT QRGVTDMLNV 450
NRCSHVAFPG ETIMQEAKLC TERYLRNALE DVGAFDKWAL KKNIRGEVEY 500
ALKYPWHRSM PRLEARSYIE HYGPNDVWLG KTMYMMPYIS NLKYLELAKL 550
DFNHVQSLHQ KELRDLRRWW KSSGLSELKF TRERVTEIYF SAASFIFEPE 600
FATCRDVYTK ISIFTVILDD LYDAHGTLDN LELFSEGVKR WDLSLVDRMP 650
QDMKICFTVL YNTVNEIAVE GRKRQGRDVL GYIRNVLEIL LAAHTKEAEW 700
SAARYVPSFD EYIENASVSI SLGTLVLISV LFTGEILTDD VLSKIGRGSR 750
FLQLMGLTGR LVNDTKTYEA ERGQGEVASA VQCYMKEHPE ISEEEALKHV 800
YTVMENALDE LNREFVNNRD VPDSCRRLVF ETARIMQLFY MEGDGLTLSH 850
EMEIKEHVKN CLFQPVA 867
Length:867
Mass (Da):99,203
Last modified:November 22, 2005 - v2
Checksum:i7E27A5BBCC6062D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY473620 mRNA. Translation: AAS47690.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY473620 mRNA. Translation: AAS47690.2 .

3D structure databases

ProteinModelPortali Q675L5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00924 .
BioCyci MetaCyc:MONOMER-12744.
BRENDAi 4.2.3.35. 4815.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily."
    Martin D.M., Faeldt J., Bohlmann J.
    Plant Physiol. 135:1908-1927(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, GENE FAMILY, NOMENCLATURE.
  2. Martin D.M., Keeling C., Bohlmann J.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiTPSD2_PICAB
AccessioniPrimary (citable) accession number: Q675L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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