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Protein

Replication-associated protein A

Gene

C1

Organism
Miscanthus streak virus (isolate 91) (MiSV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Divalent metal cationSequence analysis1
Metal bindingi71Divalent metal cationSequence analysis1
Metal bindingi73Divalent metal cationSequence analysis1
Active sitei111For DNA cleavage activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Endonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiMiscanthus streak virus (isolate 91) (MiSV)
Taxonomic identifieri268776 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiMiscanthus sacchariflorus [TaxID: 183675]
Proteomesi
  • UP000008874 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003169411 – 217Replication-associated protein AAdd BLAST217

Interactioni

Subunit structurei

Homooligomer. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ67591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 188OligomerizationBy similarityAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 33RCR-15
Motifi71 – 76RCR-26
Motifi111 – 114RCR-34

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Family and domain databases

InterProiIPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RepA (identifier: Q67591-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAPASSAAS NRPGPSNHPT PRWNSKQFFL TYPHCNLTPS ELMKELFSRL
60 70 80 90 100
TEKIPGYIKV SQEFHKDGDP HLHVLIQLNT KLCTRNPKFF DVQGFHPNIQ
110 120 130 140 150
PVRDAEKVFG YISKTNGDSD EMGELQLRIK KPEKPTRDQR MAMIIASSTN
160 170 180 190 200
RNEYLSMVRK EFPFDWAIRL QQFEYSAAAL FTEPPPVYQS PFPNEQIVCP
210
PELVDIIDQE WNQVTTD
Note: Produced from the unspliced transcript.
Length:217
Mass (Da):25,050
Last modified:November 1, 1996 - v1
Checksum:iE511C43503BFB39E
GO
Isoform Rep (identifier: Q67590-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q67590.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:348
Mass (Da):40,318
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01030 Genomic DNA. Translation: BAA00835.1.
PIRiJQ1358.
RefSeqiNP_569147.1. NC_003379.1. [Q67591-1]

Genome annotation databases

GeneIDi932215.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01030 Genomic DNA. Translation: BAA00835.1.
PIRiJQ1358.
RefSeqiNP_569147.1. NC_003379.1. [Q67591-1]

3D structure databases

ProteinModelPortaliQ67591.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi932215.

Family and domain databases

InterProiIPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.
ProtoNetiSearch...

Entry informationi

Entry nameiREPA_MISV9
AccessioniPrimary (citable) accession number: Q67591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.