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Q674R7 (ATG9B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 9B
Alternative name(s):
APG9-like 2
Nitric oxide synthase 3-overlapping antisense gene protein
Short name=Protein sONE
Gene names
Name:ATG9B
Synonyms:APG9L2, NOS3AS
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in autophagy By similarity.

Subcellular location

Cytoplasmic vesicle › autophagosome membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial. Ref.1

Induction

By hypoxia, leading to inhibit NOS3 expression. Ref.6

Miscellaneous

ATG9B gene is located on the opposite DNA strand of the NOS3 gene at chromosome 7q36. The genes are oriented in a tail-to-tail configuration and the mRNAs encoding ATG9B and NOS3 are complementary for 662 nucleotides. ATG9B transcription may a role in NOS3 transcription regulation.

Sequence similarities

Belongs to the ATG9 family.

Sequence caution

The sequence AAQ86941.1 differs from that shown. Reason: Erroneous initiation.

The sequence AC010973 differs from that shown. Reason: Frameshift at position 766.

Ontologies

Keywords
   Biological processAutophagy
   Cellular componentCytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processautophagic vacuole assembly

Inferred from direct assay Ref.4. Source: MGI

   Cellular componentautophagic vacuole membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q674R7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q674R7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-514: Missing.
     515-572: RTAAPPAPLR...TALGVTATVA → MPSYPQPSVL...CFTGRPCTSP
Note: No experimental confirmation available.
Isoform 3 (identifier: Q674R7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     322-340: EELSSVPWAEVQSRLLALQ → GKGREDTGMYWRGQPGGLD
     341-924: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 924924Autophagy-related protein 9B
PRO_0000314867

Regions

Topological domain1 – 207207Cytoplasmic By similarity
Transmembrane208 – 22821Helical; Potential
Topological domain229 – 27648Lumenal By similarity
Transmembrane277 – 29721Helical; Potential
Topological domain298 – 438141Cytoplasmic By similarity
Transmembrane439 – 45921Helical; Potential
Topological domain460 – 52667Lumenal By similarity
Transmembrane527 – 54721Helical; Potential
Topological domain548 – 5514Cytoplasmic By similarity
Transmembrane552 – 57221Helical; Potential
Topological domain573 – 62452Lumenal By similarity
Transmembrane625 – 64521Helical; Potential
Topological domain646 – 924279Cytoplasmic By similarity
Compositional bias22 – 147126Pro-rich

Natural variations

Alternative sequence1 – 514514Missing in isoform 2.
VSP_030410
Alternative sequence322 – 34019EELSS…LLALQ → GKGREDTGMYWRGQPGGLD in isoform 3.
VSP_030411
Alternative sequence341 – 924584Missing in isoform 3.
VSP_030412
Alternative sequence515 – 57258RTAAP…TATVA → MPSYPQPSVLRGSAPARCWS SSWSWLPASGWSNCFAQSAT SSATGTSRCFTGRPCTSP in isoform 2.
VSP_030413
Natural variant1661P → L.
Corresponds to variant rs61078191 [ dbSNP | Ensembl ].
VAR_061030

Experimental info

Sequence conflict121R → K in BAC04853. Ref.2
Sequence conflict7511S → P in AAQ86941. Ref.1
Sequence conflict7521T → P in AAQ86941. Ref.1
Sequence conflict7601T → A in AAQ86941. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 80C242069DF3D91E

FASTA924101,019
        10         20         30         40         50         60 
MVSRMGWGGR RRRLGRWGDL GPGSVPLLPM PLPPPPPPSC RGPGGGRISI FSLSPAPHTR 

        70         80         90        100        110        120 
SSPSSFSPPT AGPPCSVLQG TGASQSCHSA LPIPATPPTQ AQPAMTPASA SPSWGSHSTP 

       130        140        150        160        170        180 
PLAPATPTPS QQCPQDSPGL RVGPLIPEQD YERLEDCDPE GSQDSPIHGE EQQPLLHVPE 

       190        200        210        220        230        240 
GLRGSWHHIQ NLDSFFTKIY SYHQRNGFAC ILLEDVFQLG QFIFIVTFTT FLLRCVDYNV 

       250        260        270        280        290        300 
LFANQPSNHT RPGPFHSKVT LSDAILPSAQ CAERIRSSPL LVLLLVLAAG FWLVQLLRSV 

       310        320        330        340        350        360 
CNLFSYWDIQ VFYREALHIP PEELSSVPWA EVQSRLLALQ RSGGLCVQPR PLTELDIHHR 

       370        380        390        400        410        420 
ILRYTNYQVA LANKGLLPAR CPLPWGGSAA FLSRGLALNV DLLLFRGPFS LFRGGWELPH 

       430        440        450        460        470        480 
AYKRSDQRGA LAARWGRTVL LLAALNLALS PLVLAWQVLH VFYSHVELLR REPGALGARG 

       490        500        510        520        530        540 
WSRLARLQLR HFNELPHELR ARLARAYRPA AAFLRTAAPP APLRTLLARQ LVFFAGALFA 

       550        560        570        580        590        600 
ALLVLTVYDE DVLAVEHVLT AMTALGVTAT VARSFIPEEQ CQGRAPQLLL QTALAHMHYL 

       610        620        630        640        650        660 
PEEPGPGGRD RAYRQMAQLL QYRAVSLLEE LLSPLLTPLF LLFWFRPRAL EIIDFFHHFT 

       670        680        690        700        710        720 
VDVAGVGDIC SFALMDVKRH GHPQWLSAGQ TEASLSQRAE DGKTELSLMR FSLAHPLWRP 

       730        740        750        760        770        780 
PGHSSKFLGH LWGRVQQDAA AWGATSARGP STPGVLSNCT SPLPEAFLAN LFVHPLLPPR 

       790        800        810        820        830        840 
DLSPTAPCPA AATASLLASI SRIAQDPSSV SPGGTGGQKL AQLPELASAE MSLHVIYLHQ 

       850        860        870        880        890        900 
LHQQQQQQEP WGEAAASILS RPCSSPSQPP SPDEEKPSWS SDGSSPASSP RQQWGTQKAR 

       910        920 
NLFPGGFQVT TDTQKEPDRA SCTD

« Hide

Isoform 2 [UniParc].

Checksum: AFE1C6C1A626DE02
Show »

FASTA41044,503
Isoform 3 [UniParc].

Checksum: F0658A43335CB06E
Show »

FASTA34036,989

References

« Hide 'large scale' references
[1]"Post-transcriptional regulation of endothelial nitric-oxide synthase by an overlapping antisense mRNA transcript."
Robb G.B., Carson A.R., Tai S.C., Fish J.E., Singh S., Yamada T., Scherer S.W., Nakabayashi K., Marsden P.A.
J. Biol. Chem. 279:37982-37996(2004) [PubMed: 15234981] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast."
Yamada T., Carson A.R., Caniggia I., Umebayashi K., Yoshimori T., Nakabayashi K., Scherer S.W.
J. Biol. Chem. 280:18283-18290(2005) [PubMed: 15755735] [Abstract]
Cited for: IDENTIFICATION.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"Hypoxia-inducible expression of a natural cis-antisense transcript inhibits endothelial nitric-oxide synthase."
Fish J.E., Matouk C.C., Yeboah E., Bevan S.C., Khan M., Patil K., Ohh M., Marsden P.A.
J. Biol. Chem. 282:15652-15666(2007) [PubMed: 17403686] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY515311 mRNA. Translation: AAS87212.1.
AY316116 mRNA. Translation: AAQ86941.1. Different initiation.
AK096734 mRNA. Translation: BAC04853.1.
AC010973 Genomic DNA. No translation available.
BC128587 mRNA. Translation: AAI28588.1.
BK004019 mRNA. Translation: DAA05200.1.
IPIIPI00465328.
IPI00747506.
IPI00827670.
RefSeqNP_775952.4. NM_173681.5.
UniGeneHs.707300.
Hs.716846.

3D structure databases

ProteinModelPortalQ674R7.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ674R7.

Polymorphism databases

DMDM74708555.

Proteomic databases

PRIDEQ674R7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID285973.
KEGGhsa:285973.

Organism-specific databases

CTD285973.
GeneCardsGC07M150710.
H-InvDBHIX0007219.
HGNCHGNC:21899. ATG9B.
neXtProtNX_Q674R7.
PharmGKBPA134883165.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG19445.
HOGENOMHBG315812.
HOVERGENHBG050539.
InParanoidQ674R7.
PhylomeDBQ674R7.

Gene expression databases

ArrayExpressQ674R7.
CleanExHS_ATG9B.
GenevestigatorQ674R7.

Family and domain databases

InterProIPR007241. Autophagy-rel_prot_9.
[Graphical view]
PANTHERPTHR13038. APG9. 1 hit.
PfamPF04109. APG9. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio95902.

Entry information

Entry nameATG9B_HUMAN
AccessionPrimary (citable) accession number: Q674R7
Secondary accession number(s): A1A5D3, Q6JRW5, Q8N8I8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

SIMILARITY comments

Index of protein domains and families

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