Reviewed, UniProtKB/Swiss-Prot Q674R7 (ATG9B_HUMAN)

Names and origin

Protein names

Recommended name:
    Autophagy-related protein 9B
Alternative name(s):
    APG9-like 2
    Nitric oxide synthase 3-overlapping antisense gene protein
      Short name=Protein sONE

Gene names

Name:	ATG9B
Synonyms:	APG9L2, NOS3AS

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in autophagy By similarity.
Subcellular location	Cytoplasmic vesicle › autophagosome membrane; Multi-pass membrane proteinBy similarity.
Tissue specificity	Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
Induction	By hypoxia, leading to inhibit NOS3 expression.
Miscellaneous	ATG9B gene is located on the opposite DNA strand of the NOS3 gene at chromosome 7q36. The genes are oriented in a tail-to-tail configuration and the mRNAs encoding ATG9B and NOS3 are complementary for 662 nucleotides. ATG9B transcription may a role in NOS3 transcription regulation.
Sequence similarities	Belongs to the ATG9 family.
Sequence caution	The sequence AC010973 differs from that shown. Reason: Frameshift at position 766.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Gene Ontology (GO)
Biological process	autophagosome formation Ref.4 Inferred from direct assay. Source: MGI
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q674R7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q674R7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-514: Missing.
     515-572: RTAAPPAPLR...TALGVTATVA → MPSYPQPSVL...CFTGRPCTSP

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q674R7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     322-340: EELSSVPWAEVQSRLLALQ → GKGREDTGMYWRGQPGGLD
     341-924: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Autophagy-related protein 9B		PRO_0000314867
Regions
	Topological domain	1 – 207	207	Cytoplasmic By similarity
	Transmembrane	208 – 228	21	Potential
	Topological domain	229 – 276	48	Lumenal By similarity
	Transmembrane	277 – 297	21	Potential
	Topological domain	298 – 438	141	Cytoplasmic By similarity
	Transmembrane	439 – 459	21	Potential
	Topological domain	460 – 526	67	Lumenal By similarity
	Transmembrane	527 – 547	21	Potential
	Topological domain	548 – 551	4	Cytoplasmic By similarity
	Transmembrane	552 – 572	21	Potential
	Topological domain	573 – 624	52	Lumenal By similarity
	Transmembrane	625 – 645	21	Potential
	Topological domain	646 – 924	279	Cytoplasmic By similarity
	Compositional bias	22 – 147	126	Pro-rich
Natural variations
	Alternative sequence	1 – 514	514	Missing in isoform 2.		VSP_030410
	Alternative sequence	322 – 340	19	EELSS…LLALQ → GKGREDTGMYWRGQPGGLD in isoform 3.		VSP_030411
	Alternative sequence	341 – 924	584	Missing in isoform 3.		VSP_030412
	Alternative sequence	515 – 572	58	RTAAP…TATVA → MPSYPQPSVLRGSAPARCWS SSWSWLPASGWSNCFAQSAT SSATGTSRCFTGRPCTSP in isoform 2.		VSP_030413
Experimental info
	Sequence conflict	12	1	R → K in BAC04853. Ref.2
	Sequence conflict	751	1	S → P in AAQ86941. Ref.1
	Sequence conflict	752	1	T → P in AAQ86941. Ref.1
	Sequence conflict	760	1	T → A in AAQ86941. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 80C242069DF3D91E	FASTA	924	101,019
	10 20 30 40 50 60 MVSRMGWGGR RRRLGRWGDL GPGSVPLLPM PLPPPPPPSC RGPGGGRISI FSLSPAPHTR 70 80 90 100 110 120 SSPSSFSPPT AGPPCSVLQG TGASQSCHSA LPIPATPPTQ AQPAMTPASA SPSWGSHSTP 130 140 150 160 170 180 PLAPATPTPS QQCPQDSPGL RVGPLIPEQD YERLEDCDPE GSQDSPIHGE EQQPLLHVPE 190 200 210 220 230 240 GLRGSWHHIQ NLDSFFTKIY SYHQRNGFAC ILLEDVFQLG QFIFIVTFTT FLLRCVDYNV 250 260 270 280 290 300 LFANQPSNHT RPGPFHSKVT LSDAILPSAQ CAERIRSSPL LVLLLVLAAG FWLVQLLRSV 310 320 330 340 350 360 CNLFSYWDIQ VFYREALHIP PEELSSVPWA EVQSRLLALQ RSGGLCVQPR PLTELDIHHR 370 380 390 400 410 420 ILRYTNYQVA LANKGLLPAR CPLPWGGSAA FLSRGLALNV DLLLFRGPFS LFRGGWELPH 430 440 450 460 470 480 AYKRSDQRGA LAARWGRTVL LLAALNLALS PLVLAWQVLH VFYSHVELLR REPGALGARG 490 500 510 520 530 540 WSRLARLQLR HFNELPHELR ARLARAYRPA AAFLRTAAPP APLRTLLARQ LVFFAGALFA 550 560 570 580 590 600 ALLVLTVYDE DVLAVEHVLT AMTALGVTAT VARSFIPEEQ CQGRAPQLLL QTALAHMHYL 610 620 630 640 650 660 PEEPGPGGRD RAYRQMAQLL QYRAVSLLEE LLSPLLTPLF LLFWFRPRAL EIIDFFHHFT 670 680 690 700 710 720 VDVAGVGDIC SFALMDVKRH GHPQWLSAGQ TEASLSQRAE DGKTELSLMR FSLAHPLWRP 730 740 750 760 770 780 PGHSSKFLGH LWGRVQQDAA AWGATSARGP STPGVLSNCT SPLPEAFLAN LFVHPLLPPR 790 800 810 820 830 840 DLSPTAPCPA AATASLLASI SRIAQDPSSV SPGGTGGQKL AQLPELASAE MSLHVIYLHQ 850 860 870 880 890 900 LHQQQQQQEP WGEAAASILS RPCSSPSQPP SPDEEKPSWS SDGSSPASSP RQQWGTQKAR 910 920 NLFPGGFQVT TDTQKEPDRA SCTD
	Isoform 2 [UniParc]. Checksum: AFE1C6C1A626DE02		410	44,503
	10 20 30 40 50 60 MPSYPQPSVL RGSAPARCWS SSWSWLPASG WSNCFAQSAT SSATGTSRCF TGRPCTSPRS 70 80 90 100 110 120 FIPEEQCQGR APQLLLQTAL AHMHYLPEEP GPGGRDRAYR QMAQLLQYRA VSLLEELLSP 130 140 150 160 170 180 LLTPLFLLFW FRPRALEIID FFHHFTVDVA GVGDICSFAL MDVKRHGHPQ WLSAGQTEAS 190 200 210 220 230 240 LSQRAEDGKT ELSLMRFSLA HPLWRPPGHS SKFLGHLWGR VQQDAAAWGA TSARGPSTPG 250 260 270 280 290 300 VLSNCTSPLP EAFLANLFVH PLLPPRDLSP TAPCPAAATA SLLASISRIA QDPSSVSPGG 310 320 330 340 350 360 TGGQKLAQLP ELASAEMSLH VIYLHQLHQQ QQQQEPWGEA AASILSRPCS SPSQPPSPDE 370 380 390 400 410 EKPSWSSDGS SPASSPRQQW GTQKARNLFP GGFQVTTDTQ KEPDRASCTD
	Isoform 3 [UniParc]. Checksum: F0658A43335CB06E		340	36,989
	10 20 30 40 50 60 MVSRMGWGGR RRRLGRWGDL GPGSVPLLPM PLPPPPPPSC RGPGGGRISI FSLSPAPHTR 70 80 90 100 110 120 SSPSSFSPPT AGPPCSVLQG TGASQSCHSA LPIPATPPTQ AQPAMTPASA SPSWGSHSTP 130 140 150 160 170 180 PLAPATPTPS QQCPQDSPGL RVGPLIPEQD YERLEDCDPE GSQDSPIHGE EQQPLLHVPE 190 200 210 220 230 240 GLRGSWHHIQ NLDSFFTKIY SYHQRNGFAC ILLEDVFQLG QFIFIVTFTT FLLRCVDYNV 250 260 270 280 290 300 LFANQPSNHT RPGPFHSKVT LSDAILPSAQ CAERIRSSPL LVLLLVLAAG FWLVQLLRSV 310 320 330 340 CNLFSYWDIQ VFYREALHIP PGKGREDTGM YWRGQPGGLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Post-transcriptional regulation of endothelial nitric-oxide synthase by an overlapping antisense mRNA transcript." Robb G.B., Carson A.R., Tai S.C., Fish J.E., Singh S., Yamada T., Scherer S.W., Nakabayashi K., Marsden P.A. J. Biol. Chem. 279:37982-37996(2004) [PubMed: 15234981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast." Yamada T., Carson A.R., Caniggia I., Umebayashi K., Yoshimori T., Nakabayashi K., Scherer S.W. J. Biol. Chem. 280:18283-18290(2005) [PubMed: 15755735] [Abstract] Cited for: IDENTIFICATION.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]	"Hypoxia-inducible expression of a natural cis-antisense transcript inhibits endothelial nitric-oxide synthase." Fish J.E., Matouk C.C., Yeboah E., Bevan S.C., Khan M., Patil K., Ohh M., Marsden P.A. J. Biol. Chem. 282:15652-15666(2007) [PubMed: 17403686] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY515311 mRNA. Translation: AAS87212.1. AY316116 mRNA. Translation: AAQ86941.1. Different initiation. AK096734 mRNA. Translation: BAC04853.1. AC010973 Genomic DNA. No translation available. BC128587 mRNA. Translation: AAI28588.1. BK004019 mRNA. Translation: DAA05200.1.
RefSeq	NP_775952.3.
UniGene	Hs.647092
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q674R7.
Genome annotation databases
Ensembl	ENSG00000181652. Homo sapiens. [Contig view]
GeneID	285973.
KEGG	hsa:285973.
Organism-specific databases
HGNC	HGNC:21899. ATG9B.
PharmGKB	PA134883165.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q674R7.
HOVERGEN	Q674R7.
Gene expression databases
ArrayExpress	Q674R7.
CleanEx	HS_ATG9B.
Family and domain databases
InterPro	IPR007241. Autophagy_prot_9. [Graphical view]
PANTHER	PTHR13038. APG9. 1 hit.
Pfam	PF04109. APG9. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	95902.

Entry information

Entry name

ATG9B_HUMAN

Accession

Primary (citable) accession number: Q674R7
Secondary accession number(s): A1A5D3, Q6JRW5, Q8N8I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2004
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)