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Reviewed, UniProtKB/Swiss-Prot Q67480 (DNLI_FOWPV)

Last modified June 16, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
Gene names
Name: LIG
Ordered Locus Names: FPV043
ORF Names: A50R
OrganismFowlpox virus (FPV)
Taxonomic identifier10261 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus
Virus hostVertebrata [TaxID: 7742]

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Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. It is not essential for viral replication and recombination.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Divalent metal cations By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564DNA ligase
PRO_0000059591

Sites

Active site2361N6-AMP-lysine intermediate By similarity
Metal binding2881Divalent metal cation 1 By similarity
Metal binding3831Divalent metal cation 2 By similarity
Binding site2341ATP By similarity
Binding site2411ATP By similarity
Binding site2561ATP By similarity
Binding site2881ATP By similarity
Binding site3231ATP By similarity
Binding site3991ATP By similarity
Binding site4031ATP By similarity

Experimental info

Sequence conflict2811K → N in CAA82805. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q67480-1 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 85B5D829FEE89FA0

FASTA56464,487
        10         20         30         40         50         60 
MEVTLKEFRE LCTSISTESS YVKKTKLISE FIHRGRDYND VYIITKLLLP GTGKLIYNIN 

        70         80         90        100        110        120 
DKQLVKLFSK IFCHDADEMY KYVINIGDVA YVIGSFLKKS KSVVDYATES TLTLHEVDCF 

       130        140        150        160        170        180 
LTRLSTVTRE NDQIKEIKKI IPRCTPNDLR HIIRLIKHDL RMNIGPKHVL SGLHKDAYGI 

       190        200        210        220        230        240 
FKLCNNLEQV VQRSLEDNIK PLIELMVPLQ PMLASACKTF SEAVKKCPNG IIVEFKYDGE 

       250        260        270        280        290        300 
RIQIHKHDKN FKYFSRSLKP ITPHKVTDFE ELLDRAFPSA KNMILDGEII LIDTETNQPL 

       310        320        330        340        350        360 
PFGTLGINKK SMYHNACVCI FIFDCLYFND TVLIDKPLIE RRNIIHANIK EIPNRILLSE 

       370        380        390        400        410        420 
VKNISTDEEL SKLLHIVLSK NIEGFVLKDA KGVYEPGMRR WLKIKKDYLD GCVMADKADL 

       430        440        450        460        470        480 
VVLGAYYGKG NKSGILSSFL MGCYDTKSEK WCTVTKCSGG HTDLELQEIN DNLSVVPFDR 

       490        500        510        520        530        540 
NAIPDWLSIN KIHYPDVIIS DISLAPVWEI IGSEFTRSST HTASNISIRF PRCSRIREDK 

       550        560 
TYETANNLND IKQLYAVSIS PPEE

« Hide

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References

« Hide 'large scale' references
[1]"Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes."
Skinner M.A., Moore J.B., Binns M.M., Smith G.L., Boursnell M.E.G.
J. Gen. Virol. 75:2495-2498(1994) [PubMed: 8077953] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FP-9 / Isolate HP-438.
[2]"The genome of fowlpox virus."
Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.
J. Virol. 74:3815-3831(2000) [PubMed: 10729156] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

Z29716 Genomic DNA. Translation: CAA82805.1.
AF198100 Genomic DNA. Translation: AAF44387.1.
PIRS41974.
RefSeqNP_039006.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1486591.

Enzyme and pathway databases

BRENDA6.5.1.1. 288568.

Family and domain databases

InterProIPR000977. DNA_ligase.
IPR012309. DNA_ligase_A_C.
IPR012310. DNA_ligase_A_M.
IPR012308. DNA_ligase_A_N.
IPR016059. DNA_ligase_CS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. False negative.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDNLI_FOWPV
AccessionPrimary (citable) accession number: Q67480
Secondary accession number(s): Q9J5G0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 29, 2001
Last modified: June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents