Reviewed, UniProtKB/Swiss-Prot Q67480 (DNLI_FOWPV)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	LIG
Ordered Locus Names:	FPV043
ORF Names:	A50R

Organism

Fowlpox virus (FPV)

Taxonomic identifier

10261 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA. It is not essential for viral replication and recombination.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	DNA ligase		PRO_0000059591
Sites
	Active site	236	1	N6-AMP-lysine intermediate By similarity
Experimental info
	Sequence conflict	281	1	K → N in CAA82805. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q67480-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 85B5D829FEE89FA0	FASTA	564	64,487
	10 20 30 40 50 60 MEVTLKEFRE LCTSISTESS YVKKTKLISE FIHRGRDYND VYIITKLLLP GTGKLIYNIN 70 80 90 100 110 120 DKQLVKLFSK IFCHDADEMY KYVINIGDVA YVIGSFLKKS KSVVDYATES TLTLHEVDCF 130 140 150 160 170 180 LTRLSTVTRE NDQIKEIKKI IPRCTPNDLR HIIRLIKHDL RMNIGPKHVL SGLHKDAYGI 190 200 210 220 230 240 FKLCNNLEQV VQRSLEDNIK PLIELMVPLQ PMLASACKTF SEAVKKCPNG IIVEFKYDGE 250 260 270 280 290 300 RIQIHKHDKN FKYFSRSLKP ITPHKVTDFE ELLDRAFPSA KNMILDGEII LIDTETNQPL 310 320 330 340 350 360 PFGTLGINKK SMYHNACVCI FIFDCLYFND TVLIDKPLIE RRNIIHANIK EIPNRILLSE 370 380 390 400 410 420 VKNISTDEEL SKLLHIVLSK NIEGFVLKDA KGVYEPGMRR WLKIKKDYLD GCVMADKADL 430 440 450 460 470 480 VVLGAYYGKG NKSGILSSFL MGCYDTKSEK WCTVTKCSGG HTDLELQEIN DNLSVVPFDR 490 500 510 520 530 540 NAIPDWLSIN KIHYPDVIIS DISLAPVWEI IGSEFTRSST HTASNISIRF PRCSRIREDK 550 560 TYETANNLND IKQLYAVSIS PPEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes." Skinner M.A., Moore J.B., Binns M.M., Smith G.L., Boursnell M.E.G. J. Gen. Virol. 75:2495-2498(1994) [PubMed: 8077953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FP-9 / Isolate HP-438.
[2]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed: 10729156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z29716 Genomic DNA. Translation: CAA82805.1. AF198100 Genomic DNA. Translation: AAF44387.1.
PIR	S41974.
RefSeq	NP_039006.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1486591.
Family and domain databases
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_FOWPV

Accession

Primary (citable) accession number: Q67480
Secondary accession number(s): Q9J5G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 29, 2001
Last modified:	November 25, 2008
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)