Q67477 (3BHS_FOWPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
Short name=3-beta-HSD

Including the following 2 domains:

1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
   EC=1.1.1.145
   Alternative name(s):
   3-beta-hydroxy-5-ene steroid dehydrogenase
   Progesterone reductase
2. Steroid Delta-isomerase
   EC=5.3.3.1
   Alternative name(s):
   Delta-5-3-ketosteroid isomerase

Gene names

Ordered Locus Names:

FPV046

Organism

Fowlpox virus (strain NVSL) (FPV)

Taxonomic identifier

928301 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids By similarity.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase		PRO_0000087797
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity
Experimental info
	Sequence conflict	38 – 43	6	IRIDQW → LDRPM in CAA82802. Ref.1
	Sequence conflict	251	1	N → K in CAA82802. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q67477 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 730FE72BA84FA681	FASTA	370	42,349
	10 20 30 40 50 60 MRTLVYVVTG GCGFLGRHII NNLILFESSL KEVRVYDIRI DQWLLDLVEK CNIIKIVPVI 70 80 90 100 110 120 GDVRNKSTLD EALRSADVVI HIASINDVAG KFTNDSIMDV NINGTKNVVD SCLYNGVRVL 130 140 150 160 170 180 VYTSSYSAVG PNFLGDAMIR GNENTYYQSN HKEAYPLSKQ LSEKYILEAN GTMSNIGLRL 190 200 210 220 230 240 CTCALRPLGV FGEYCPVLET LYRRSYKSRK MYKYADDKVF HSRVYAGNVA WMHILAARNM 250 260 270 280 290 300 IENGQHSPLC NNVYYCYDTS PTEHYHDFNM HFFNQLGMDL RNTCLPLWCL RFIANINKGL 310 320 330 340 350 360 RVLLSPICSY TPLLNPYTLI KECTTFTIET DKAFKDFGYV PLYTWEESRS KTQLWIRELE 370 AKSSSQKPKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes." Skinner M.A., Moore J.B., Binns M.M., Smith G.L., Boursnell M.E.G. J. Gen. Virol. 75:2495-2498(1994) [PubMed: 8077953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FP-9 / Isolate HP-438.
[2]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed: 10729156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z29716 Genomic DNA. Translation: CAA82802.1. AF198100 Genomic DNA. Translation: AAF44390.1.
PIR	S41971.
RefSeq	NP_039008.1. NC_002188.1.
3D structure databases
ProteinModelPortal	Q67477.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486594.
Phylogenomic databases
ProtClustDB	CLSP2509841.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3BHS_FOWPN

Accession

Primary (citable) accession number: Q67477
Secondary accession number(s): Q9J5F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	December 14, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program