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Q67387

- HEMA_INCNB

UniProt

Q67387 - HEMA_INCNB

Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene

HE

Organism
Influenza C virus (strain C/Nara/2/1985)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei64 – 641NucleophileBy similarity
    Active sitei359 – 3591Charge relay systemBy similarity
    Active sitei362 – 3621Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. fusion of virus membrane with host plasma membrane Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
    Short name:
    HEF
    Cleaved into the following 2 chains:
    Gene namesi
    Name:HE
    OrganismiInfluenza C virus (strain C/Nara/2/1985)
    Taxonomic identifieri127957 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 77By similarity
    Chaini8 – 439432Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039160Add
    BLAST
    Chaini440 – 648209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039161Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi13 ↔ 576Interchain (between HEF1 and HEF2 chains)By similarity
    Glycosylationi19 – 191N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi113 ↔ 158By similarity
    Disulfide bondi133 ↔ 181By similarity
    Glycosylationi137 – 1371N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi203 ↔ 245By similarity
    Disulfide bondi222 ↔ 309By similarity
    Disulfide bondi230 ↔ 282By similarity
    Disulfide bondi339 ↔ 345By similarity
    Glycosylationi388 – 3881N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HEF1-HEF2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ67387.
    SMRiQ67387. Positions 8-434, 443-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini8 – 623616ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini645 – 6484CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei624 – 64421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 3326Fusion domain-1By similarityAdd
    BLAST
    Regioni34 – 144111Esterase domain-1By similarityAdd
    BLAST
    Regioni144 – 303160N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd
    BLAST
    Regioni304 – 35855Esterase domain-2By similarityAdd
    BLAST
    Regioni359 – 644286Fusion domain-2By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.90.20.10. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q67387-1 [UniParc]FASTAAdd to Basket

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    MLGLTEAEKI KICLQKQVNS SFSLHNGFGG NLYATEEKRM FELVKPKAGA    50
    SVLNQSTWIG FGDSRTDQSN SAFPRSADVS AKTADKFRSL SGGSLMLSMF 100
    GPPGKVDYLY QGCGKHKVFY EGVNWSPHAA IDCYRKNWTD IKLNFQKSIY 150
    ELASQSHCMS LVNALDKTIP LQVTKGVAKN CNNSFLKNPA LYTQEVKPLE 200
    QICGKENLAF FTLPTQFGTY ECKLHLVASC YFIYDSKEVY NKRGCGNYFQ 250
    VIYDSSGKVV GGLDNRVSPY TGNSGDTPTM QCDMLQLKPG RYSVRSSPRF 300
    LLMPERSYCF DMKEKGPVTA VQSIWGKGRE SDYAVDQACL STPGCMLIQK 350
    QKPYIGEADD HHGDQEMREL LSGLDYEARC ISQSGWVNET SPFTEEYLLP 400
    PKFGRCPLAA KEESIPKIPD GLLIPTSGTD TTVTKPKSRI FGIDDLIIGL 450
    LFVAIVEAGI GGYLLGSRKE SGGGVTKESA EKGFEKIGND IQILRSSTNI 500
    AIEKLNDRIS HDEQAIRDLT LEIENARSEA LLGELGIIRA LLVGNISIGL 550
    QESLWELASE ITNRAGDLAV EVSPGCWIID NNICDQSCQN FIFKFNETAP 600
    VPTIPPLDTK IDLQSDPFYW GSSLGLAITA AISLAALVIS GIAICRTK 648
    Length:648
    Mass (Da):71,229
    Last modified:November 1, 1996 - v1
    Checksum:iD6736996092E5DB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30697 Genomic RNA. Translation: BAA06368.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30697 Genomic RNA. Translation: BAA06368.1 .

    3D structure databases

    ProteinModelPortali Q67387.
    SMRi Q67387. Positions 8-434, 443-604.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.90.20.10. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic reassortment of influenza C viruses in man."
      Peng G., Hongo S., Muraki Y., Sugawara K., Nishimura H., Kitame F., Nakamura K.
      J. Gen. Virol. 75:3619-3622(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_INCNB
    AccessioniPrimary (citable) accession number: Q67387
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3