SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q67387

- HEMA_INCNB

UniProt

Q67387 - HEMA_INCNB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Hemagglutinin-esterase-fusion glycoprotein
Gene
HE
Organism
Influenza C virus (strain C/Nara/2/1985)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Nucleophile By similarity
Active sitei359 – 3591Charge relay system By similarity
Active sitei362 – 3621Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/Nara/2/1985)
Taxonomic identifieri127957 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini8 – 623616Extracellular Reviewed prediction
Add
BLAST
Transmembranei624 – 64421Helical; Reviewed prediction
Add
BLAST
Topological domaini645 – 6484Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 77 By similarity
Chaini8 – 439432Hemagglutinin-esterase-fusion glycoprotein chain 1
PRO_0000039160Add
BLAST
Chaini440 – 648209Hemagglutinin-esterase-fusion glycoprotein chain 2
PRO_0000039161Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi13 ↔ 576Interchain (between HEF1 and HEF2 chains) By similarity
Glycosylationi19 – 191N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi54 – 541N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi113 ↔ 158 By similarity
Disulfide bondi133 ↔ 181 By similarity
Glycosylationi137 – 1371N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi203 ↔ 245 By similarity
Disulfide bondi222 ↔ 309 By similarity
Disulfide bondi230 ↔ 282 By similarity
Disulfide bondi339 ↔ 345 By similarity
Glycosylationi388 – 3881N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ67387.
SMRiQ67387. Positions 8-434, 443-604.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 3326Fusion domain-1 By similarity
Add
BLAST
Regioni34 – 144111Esterase domain-1 By similarity
Add
BLAST
Regioni144 – 303160N-acetyl-9-O-acetylneuraminic acid binding By similarity
Add
BLAST
Regioni304 – 35855Esterase domain-2 By similarity
Add
BLAST
Regioni359 – 644286Fusion domain-2 By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q67387-1 [UniParc]FASTAAdd to Basket

« Hide

MLGLTEAEKI KICLQKQVNS SFSLHNGFGG NLYATEEKRM FELVKPKAGA    50
SVLNQSTWIG FGDSRTDQSN SAFPRSADVS AKTADKFRSL SGGSLMLSMF 100
GPPGKVDYLY QGCGKHKVFY EGVNWSPHAA IDCYRKNWTD IKLNFQKSIY 150
ELASQSHCMS LVNALDKTIP LQVTKGVAKN CNNSFLKNPA LYTQEVKPLE 200
QICGKENLAF FTLPTQFGTY ECKLHLVASC YFIYDSKEVY NKRGCGNYFQ 250
VIYDSSGKVV GGLDNRVSPY TGNSGDTPTM QCDMLQLKPG RYSVRSSPRF 300
LLMPERSYCF DMKEKGPVTA VQSIWGKGRE SDYAVDQACL STPGCMLIQK 350
QKPYIGEADD HHGDQEMREL LSGLDYEARC ISQSGWVNET SPFTEEYLLP 400
PKFGRCPLAA KEESIPKIPD GLLIPTSGTD TTVTKPKSRI FGIDDLIIGL 450
LFVAIVEAGI GGYLLGSRKE SGGGVTKESA EKGFEKIGND IQILRSSTNI 500
AIEKLNDRIS HDEQAIRDLT LEIENARSEA LLGELGIIRA LLVGNISIGL 550
QESLWELASE ITNRAGDLAV EVSPGCWIID NNICDQSCQN FIFKFNETAP 600
VPTIPPLDTK IDLQSDPFYW GSSLGLAITA AISLAALVIS GIAICRTK 648
Length:648
Mass (Da):71,229
Last modified:November 1, 1996 - v1
Checksum:iD6736996092E5DB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30697 Genomic RNA. Translation: BAA06368.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30697 Genomic RNA. Translation: BAA06368.1 .

3D structure databases

ProteinModelPortali Q67387.
SMRi Q67387. Positions 8-434, 443-604.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic reassortment of influenza C viruses in man."
    Peng G., Hongo S., Muraki Y., Sugawara K., Nishimura H., Kitame F., Nakamura K.
    J. Gen. Virol. 75:3619-3622(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCNB
AccessioniPrimary (citable) accession number: Q67387
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi