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Q672W7 (Q672W7_HELPX) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase HAMAP-Rule MF_00163

Short name=PDF HAMAP-Rule MF_00163
EC=3.5.1.88 HAMAP-Rule MF_00163
Alternative name(s):
Polypeptide deformylase HAMAP-Rule MF_00163
Gene names
Name:def HAMAP-Rule MF_00163 EMBL AAT51863.1
OrganismHelicobacter pylori (Campylobacter pylori) EMBL AAT51863.1
Taxonomic identifier210 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length174 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. SAAS SAAS000181 HAMAP-Rule MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP-Rule MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family. RuleBase RU003335 HAMAP-Rule MF_00163

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1391 By similarity HAMAP-Rule MF_00163
Metal binding961Cobalt PDB 2EW5 PDB 2EW7 PDB 2EW6 PDB 4E9A PDB 4E9B
Metal binding961Iron By similarity HAMAP-Rule MF_00163
Metal binding1381Cobalt; via tele nitrogen PDB 2EW5 PDB 2EW7 PDB 2EW6 PDB 4E9A PDB 4E9B
Metal binding1381Iron By similarity HAMAP-Rule MF_00163
Metal binding1421Cobalt; via tele nitrogen PDB 2EW5 PDB 2EW7 PDB 2EW6 PDB 4E9A PDB 4E9B
Metal binding1421Iron By similarity HAMAP-Rule MF_00163

Sequences

Sequence LengthMass (Da)Tools
Q672W7 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: A10BEAEBE6CC4C85

FASTA17420,038
        10         20         30         40         50         60 
MALLEIIHYP SKILRTISKE VVSFDAKLHQ QLDDMYETMI ASEGIGLAAI QVGLPLRMLI 

        70         80         90        100        110        120 
INLPQEDGVQ HKEDCLEIIN PKFIETGGSM MYKEGCLSVP GFYEEVERFE KVKIEYQNRF 

       130        140        150        160        170 
AEVKVLEASE LLAVAIQHEI DHLNGVLFVD KLSILKRKKF EKELKELQKK QKHK 

« Hide

References

[1]"Molecular cloning and characterization of a new peptide deformylase from human pathogenic bacterium Helicobacter pylori."
Han C., Wang Q., Dong L., Sun H., Peng S., Chen J., Yang Y., Yue J., Shen X., Jiang H.
Biochem. Biophys. Res. Commun. 319:1292-1298(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: SS1 EMBL AAT51863.1.
[2]"Peptide deformylase is a potential target for anti-Helicobacter pylori drugs: reverse docking, enzymatic assay, and X-ray crystallography validation."
Cai J., Han C., Hu T., Zhang J., Wu D., Wang F., Liu Y., Ding J., Chen K., Yue J., Shen X., Jiang H.
Protein Sci. 15:2071-2081(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-174 IN COMPLEX WITH COBALT.
[3]"Identification of Novel Peptide Deformylase Inhibitors from Natural Products."
Cui K., Zhu L., Lu W., Huang J.
Submitted (MAR-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 2-174 IN COMPLEX WITH COBALT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY559449 Genomic DNA. Translation: AAT51863.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EW5X-ray2.20A2-174[»]
2EW6X-ray2.20A2-174[»]
2EW7X-ray2.20A2-174[»]
4E9AX-ray1.66A2-174[»]
4E9BX-ray1.70A2-164[»]
ProteinModelPortalQ672W7.
SMRQ672W7. Positions 2-167.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.45.10. 1 hit.
HAMAPMF_00163. Pep_deformylase.
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERPTHR10458. PTHR10458. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. SSF56420. 1 hit.
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ672W7.

Entry information

Entry nameQ672W7_HELPX
AccessionPrimary (citable) accession number: Q672W7
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)