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Protein

NADPH oxidase 3

Gene

Nox3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity (By similarity).By similarity

Enzyme regulationi

Activated by the ototoxic drug cisplatin. Activated by NOXO1. Cooperatively activated by NCF1 and NCF2 or NOXA1 in a phorbol 12-myristate 13-acetate (PMA)-dependent manner. Inhibited by diphenyleneiodonium chloride (By similarity).By similarity

GO - Molecular functioni

  • oxidoreductase activity Source: RGD
  • superoxide-generating NADPH oxidase activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BRENDAi1.6.3.1. 5301.
ReactomeiR-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Protein family/group databases

PeroxiBasei5407. RnoNOx03.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase 3 (EC:1.6.3.-)
Gene namesi
Name:Nox3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1303190. Nox3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST
Topological domaini34 – 4916ExtracellularSequence analysisAdd
BLAST
Transmembranei50 – 7021HelicalSequence analysisAdd
BLAST
Topological domaini71 – 10333CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 16743ExtracellularSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Topological domaini189 – 20113CytoplasmicSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Topological domaini223 – 395173ExtracellularSequence analysisAdd
BLAST
Transmembranei396 – 41621HelicalSequence analysisAdd
BLAST
Topological domaini417 – 568152CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • extracellular exosome Source: Ensembl
  • membrane Source: RGD
  • NADPH oxidase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568NADPH oxidase 3PRO_0000227598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ672K1.
PRIDEiQ672K1.

Expressioni

Tissue specificityi

Expressed in the inner ear by the spiral glanglia and the organ of Corti.1 Publication

Interactioni

Subunit structurei

Interacts with and stabilizes CYBA/p22phox.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022148.

Structurei

3D structure databases

ProteinModelPortaliQ672K1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 284230Ferric oxidoreductaseAdd
BLAST
Domaini285 – 395111FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiQ672K1.
KOiK08008.
OMAiWICRDPS.
OrthoDBiEOG71P299.
PhylomeDBiQ672K1.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR029653. NOX3.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF12. PTHR11972:SF12. 2 hits.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q672K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTCWILNES VSFVVALLWL AINIYLFIDT FCWYAEEESF FYTRVILGSA
60 70 80 90 100
LAWARASAVC LNFNCMLILL PVSRNFVSLV RGTSVCCRGP WRRQLDKNLK
110 120 130 140 150
FHKLVAYGIA VNSVIHIVAH LFNLERYHLG QAKDAEGLLA ALSKLGNAPN
160 170 180 190 200
ESYLNPVRTL YTGTTTQLLM TVSGITGLVI SLALILIMTS STEFIRQSSY
210 220 230 240 250
ELFWYTHHIF IFLFISLAIH GGGRIIRGQT PESLRLHNVT FCRDHFDEWQ
260 270 280 290 300
EAASCPVPQF SGKEPSAWKW TLGPVVLYAC EIIIRFWRSH QEVVITKVVS
310 320 330 340 350
HPSAVLELHM KKRDFKMAPG QYIFIQCPSI SPLEWHPFTL TSAPQEDFFS
360 370 380 390 400
VHIRASGDWT EALLKAFGAE GQAPSELCSM PRLAVDGPFG GSLADVFHYP
410 420 430 440 450
VSVCIATGIG VTPFASLLKS VWYKCCESQS LPGLSKVYFY WICRDAAAFE
460 470 480 490 500
WFADLLLSLE TQMSEQGKAH LLSYHIYLTG WDEYQAIHIA LHWDESLDVI
510 520 530 540 550
TGLKQKTFYG RPNWNEEFKQ IAYNHPSSSI GVFFCGPKAM SKTLQKMCRL
560
YSSSDPRGVH FYYNKENF
Length:568
Mass (Da):64,416
Last modified:October 11, 2004 - v1
Checksum:i1E19B013E7320EE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY573239 mRNA. Translation: AAT80343.1.
RefSeqiNP_001004216.1. NM_001004216.1.
UniGeneiRn.161761.

Genome annotation databases

EnsembliENSRNOT00000022148; ENSRNOP00000022148; ENSRNOG00000016490.
GeneIDi292279.
KEGGirno:292279.
UCSCiRGD:1303190. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY573239 mRNA. Translation: AAT80343.1.
RefSeqiNP_001004216.1. NM_001004216.1.
UniGeneiRn.161761.

3D structure databases

ProteinModelPortaliQ672K1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022148.

Protein family/group databases

PeroxiBasei5407. RnoNOx03.

Proteomic databases

PaxDbiQ672K1.
PRIDEiQ672K1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022148; ENSRNOP00000022148; ENSRNOG00000016490.
GeneIDi292279.
KEGGirno:292279.
UCSCiRGD:1303190. rat.

Organism-specific databases

CTDi50508.
RGDi1303190. Nox3.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiQ672K1.
KOiK08008.
OMAiWICRDPS.
OrthoDBiEOG71P299.
PhylomeDBiQ672K1.

Enzyme and pathway databases

BRENDAi1.6.3.1. 5301.
ReactomeiR-RNO-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

PROiQ672K1.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR029653. NOX3.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF12. PTHR11972:SF12. 2 hits.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Inner ear.

Entry informationi

Entry nameiNOX3_RAT
AccessioniPrimary (citable) accession number: Q672K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.