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Protein

NADPH oxidase 3

Gene

Nox3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.2 Publications

Enzyme regulationi

Activated by the ototoxic drug cisplatin. Activated by NOXO1. Cooperatively activated by NCF1 and NCF2 or NOXA1 in a phorbol 12-myristate 13-acetate (PMA)-dependent manner. Inhibited by diphenyleneiodonium chloride.1 Publication

GO - Molecular functioni

  • superoxide-generating NADPH oxidase activity Source: MGI

GO - Biological processi

  • detection of gravity Source: MGI
  • otolith development Source: MGI
  • response to gravity Source: MGI
  • superoxide anion generation Source: MGI
  • temperature homeostasis Source: MGI

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BRENDAi1.6.3.1 3474
ReactomeiR-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Protein family/group databases

PeroxiBasei5959 MmNOx03

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase 3 (EC:1.6.3.-)
Gene namesi
Name:Nox3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2681162 Nox3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 49ExtracellularSequence analysisAdd BLAST16
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 103CytoplasmicSequence analysisAdd BLAST33
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 167ExtracellularSequence analysisAdd BLAST43
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 201CytoplasmicSequence analysisAdd BLAST13
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Topological domaini223 – 395ExtracellularSequence analysisAdd BLAST173
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Topological domaini417 – 568CytoplasmicSequence analysisAdd BLAST152

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display balance defects, a tilted position of the head and abnormal performances in motor coordination tests. This is associated with the absence of otoconia in both the utricle and saccule of the inner ear.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi408G → R: Defects in otoconia biogenesis. 1 Publication1
Mutagenesisi542K → E: Defects in otoconia biogenesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002275971 – 568NADPH oxidase 3Add BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ672J9
PRIDEiQ672J9

PTM databases

iPTMnetiQ672J9
PhosphoSitePlusiQ672J9

Expressioni

Tissue specificityi

Specifically expressed in inner ear by the spiral glanglion neurons, the vestibular system and the sensory epithelial cell layer of the saccule. Weakly expressed in skull and brain.1 Publication

Developmental stagei

Expressed in fetal kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000023802
CleanExiMM_NOX3

Interactioni

Subunit structurei

Interacts with and stabilizes CYBA/p22phox.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024565

Structurei

3D structure databases

ProteinModelPortaliQ672J9
SMRiQ672J9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 284Ferric oxidoreductaseAdd BLAST230
Domaini285 – 395FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST111

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
GeneTreeiENSGT00550000074350
HOGENOMiHOG000216669
HOVERGENiHBG003760
InParanoidiQ672J9
KOiK21422
OMAiGCWILNE
OrthoDBiEOG091G09RV
PhylomeDBiQ672J9
TreeFamiTF105354

Family and domain databases

InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR029653 NOX3
IPR017938 Riboflavin_synthase-like_b-brl
PANTHERiPTHR11972:SF12 PTHR11972:SF12, 1 hit
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Q672J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVCWILNES GSFVVALLWL AVNAYLFIDT FFWYTEEEAF FYTRVILGSA
60 70 80 90 100
LAWARASAVC LNFNCMLILL PVSRNFISLV RGTSVCCRGP WRRQLDKNLN
110 120 130 140 150
FHKLVAYGIA VNSVIHIVAH LFNLERYHLG QAKDAEGLLA ALSKLGDAPN
160 170 180 190 200
ESYLNPVRTF DMGTTTELLM TVSGITGLGI SLALVFIMTS STEFIRRSSY
210 220 230 240 250
ELFWYTHHIF VFFFISLAIH GGGRIIRGQT PESLRLHNVT YCRDHYAEWQ
260 270 280 290 300
AAALCPVPQF SGKEPSAWKW ALGPVVLYAC ERIIRFWRSH QEVVITKVVS
310 320 330 340 350
HPSAVLELHM KKRDFKMAPG QYIFIQCPSV SPLEWHPFTL TSAPQEDFFS
360 370 380 390 400
VHIRASGDWT EALLKAFRVE GQAPSELCSM PRLAVDGPFG GSLADVFHYP
410 420 430 440 450
VSVCIATGIG VTPFASLLKS VWYKCCESQS LPELSKVYFY WICRDAGAFE
460 470 480 490 500
WFADLLLSLE TRMSEQGKAH LLSYHIYLTG WDENQAIHIA LHWDESLDVI
510 520 530 540 550
TGLKQKAFYG RPNWNDEFKQ IAYNHPSSSI GVFFCGSKAM SKTLQKMCRL
560
YSSVDPRGVH FYYNKENF
Length:568
Mass (Da):64,495
Last modified:March 21, 2006 - v2
Checksum:i345FD67494ADB8F2
GO

Sequence cautioni

The sequence AAI06863 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAO65981 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161D → Y in AAT80344 (PubMed:15326186).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY182377 mRNA Translation: AAO65981.1 Different initiation.
AY573240 mRNA Translation: AAT80344.1
BC106862 mRNA Translation: AAI06863.1 Different initiation.
CCDSiCCDS28364.2
RefSeqiNP_945196.2, NM_198958.2
UniGeneiMm.328756

Genome annotation databases

EnsembliENSMUST00000115800; ENSMUSP00000111466; ENSMUSG00000023802
GeneIDi224480
KEGGimmu:224480
UCSCiuc008aev.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNOX3_MOUSE
AccessioniPrimary (citable) accession number: Q672J9
Secondary accession number(s): Q6Y4Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: February 28, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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