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Q672I1

- POLG_SVSAP

UniProt

Q672I1 - POLG_SVSAP

Protein

Genome polyprotein

Gene
N/A
Organism
Sapporo virus (isolate GI/Human/Germany/pJG-Sap01) (Hu/Dresden/pJG-Sap01/DE)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.By similarity
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
    Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved By similarity. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs By similarity.By similarity
    Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.By similarity

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei68 – 692Cleavage; by Pro-PolBy similarity
    Sitei324 – 3252Cleavage; by Pro-PolBy similarity
    Sitei665 – 6662Cleavage; by Pro-PolBy similarity
    Sitei939 – 9402Cleavage; by Pro-PolBy similarity
    Sitei1053 – 10542Cleavage; by Pro-PolBy similarity
    Active sitei1084 – 10841For protease activityBy similarity
    Active sitei1105 – 11051For protease activityBy similarity
    Active sitei1169 – 11691For protease activityBy similarity
    Sitei1721 – 17222Cleavage; by Pro-PolBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi480 – 4878ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. RNA-protein covalent cross-linking Source: UniProtKB-KW
    3. transcription, DNA-templated Source: InterPro
    4. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    DNA replication, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiC24.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 7 chains:
    Alternative name(s):
    p35
    Alternative name(s):
    VPg
    p14
    Protease-polymerase p70 (EC:2.7.7.48, EC:3.4.22.66)
    Short name:
    Pro-Pol
    Capsid protein
    Short name:
    CP
    Alternative name(s):
    VP1
    p60
    OrganismiSapporo virus (isolate GI/Human/Germany/pJG-Sap01) (Hu/Dresden/pJG-Sap01/DE)
    Taxonomic identifieri291175 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeSapovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007049: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22802280Genome polyproteinPRO_0000342108Add
    BLAST
    Chaini1 – 6868Protein p11PRO_0000342109Add
    BLAST
    Chaini69 – 324256Protein p28PRO_0000342110Add
    BLAST
    Chaini325 – 665341NTPasePRO_0000342111Add
    BLAST
    Chaini666 – 939274Protein p32PRO_0000342112Add
    BLAST
    Chaini940 – 1053114Viral genome-linked proteinPRO_0000342113Add
    BLAST
    Chaini1054 – 1721668Protease-polymerase p70PRO_0000342114Add
    BLAST
    Chaini1722 – 2280559Capsid proteinPRO_0000342115Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei965 – 9651O-(5'-phospho-RNA)-tyrosineBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein.
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Interactioni

    Subunit structurei

    Capsid protein homodimerizes, then multimerizes.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ672I1.
    SMRiQ672I1. Positions 1207-1705.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini454 – 608155SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1069 – 1175107Peptidase C24Add
    BLAST
    Domaini1442 – 1567126RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently.

    Sequence similaritiesi

    Contains 1 peptidase C24 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative promoter usage. Align

    Isoform Genome polyprotein (identifier: Q672I1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSKPFKPIV LNATFEWQVF KRCYLRVAPR EAFCENLSEL HHYFARRVNA     50
    WLKHATRTLP DGYTFVEEGL LDMFGTKAPD SVQEGTLFRE LFGVDQTEQF 100
    PLSLADLAKL QGELVDATRT PGHALRQKYT MTTIQDLINK ITKVVPVQAT 150
    LTEMHARRQF ERERADLFHE LPLVDEDAVS QPKTYFYTMW RQVVKKGKAY 200
    FCPLVKTSAW RTKISAITEP IKDFLIAFCQ AVQQEMGVNP QYLQLAWLQK 250
    LKPTTLTIIL QQHKYTVSGW LATMTALVEV YSNLFDDLRK SSVAIVSSIG 300
    AFFDICKDFV SQVVELVKTT FTAQGPTDLG WAAVLAGAAM ILLKMSGCPG 350
    VIGMWTKVLK ICGGITTITA AARGVRWLKD LYEEAEGRRL AKMYMARGAA 400
    LIELAASREV TGIDELKGLL DCFTILIEEG TELIHKFGTS PLAGLVRTYV 450
    SELETQANNI RSTIKLDTPR RVPVVIILTG APGIGKTRLA QYVGQRFGKT 500
    SNFSVAVDHH DGYTGNTVCI WDEFDVDSKG AFVETMIGIA NTAPFPLNCD 550
    RVENKGRVFT SDYVICTSNY PTSVIPDNPR AAAFYRRVLT VDVSAPDLEE 600
    WKKRNPGKRP TPDLYQDDFS HLKLMLRPYL GYNPDGDTLE GPRVAPTQIS 650
    IAGLITLMER RFKEQAGPLQ NLWLQVPKTL VEQSTNMVKA FMYANRAVCD 700
    VIPNPATRDI AETALTKIFV CGTAPPPEFV GKHIVITGIE VGDASIANSL 750
    LSMFTTTTRL SAAAQREYMY RVWSPLIHIQ DRSINTQNLP YINRVIPVTS 800
    HWDFLRGLRH HLGFTSIPGM WKAFQGWRTS QGIVDFVAHH MADVTFPSNP 850
    ECTIFRTPDA DVVFYTFGSY VCFATPARVP YVGTPPTTIH SNTPRCMTWG 900
    ETIALLCEVV AEFVLHFGPV ILSAANIAYL MTRGSRTEEA KGKTKHGRGM 950
    RHGHRAGVSL SDDEYDEWRD LMRDWRRDMS VNDFLMLRER SALGVDDEDE 1000
    ARYRAWLEIR AMRMAGGAYT HATIIGRGGV RDEIIRTAPR RAPTRPQQHY 1050
    EEEAPTAIVE FTQGGDHIGY GVHIGNGNVI TVTHVASTSD EVNGSAFKIT 1100
    RTVGETTWVQ GPFSQLPHMQ IGSGSPVYFT TRLHPVFTIS EGTFETPNIT 1150
    VNGFHVRIMN GYPTKKGDCG LPYFNSNRQL VALHAGTDTQ GETKVAQRVV 1200
    KEVTTQDEFQ WKGLPVVKSG LDVGGMPTGT RYHRSPAWPE EQPGETHAPA 1250
    PFGAGDKRYT FSQTEMLVNG LKPYTEPTAG VPPQLLSRAV THVRSYIETI 1300
    IGTHRSPVLT YHQACELLER TTSCGPFVQG LKGDYWDEEQ QQYTGVLANH 1350
    LEQAWDKANK GIAPRNAYKL ALKDELRPIE KNKAGKRRLL WGCDAATTLI 1400
    ATAAFKAVAT RLQVVTPMTP VAVGINMDSV QMQVMNDSLK GGVLYCLDYS 1450
    KWDSTQNPAV TAASLAILER FAEPHPIVSC AIEALSSPAE GYVNDIKFVT 1500
    RGGLPSGMPF TSVVNSINHM IYVAAAILQA YESHNVPYTG NVFQVETVHT 1550
    YGDDCMYSVC PATASIFHAV LANLTSYGLK PTAADKSDAI KPTNTPVFLK 1600
    RTFTQTPHGV RALLDITSIT RQFYWLKANR TSDPSSPPAF DRQARSAQLE 1650
    NALAYASQHG PVVFDTVRQI AIKTAQGEGL VLVNTNYDQA LATYNAWFIG 1700
    GTVPDPVGHT EGTHKIVFEM EGNGSNPEPK QSNNPMVVDP PGTTGPTTSH 1750
    VVVANPEQPN GAAQRLELAV ATGAIQSNVP EAIRNCFAVF RTFAWNDRMP 1800
    TGTFLGSISL HPNINPYTAH LSGMWAGWGG SFEVRLSISG SGVFAGRIIA 1850
    SVIPPGVDPS SIRDPGVLPH AFVDARITEP VSFMIPDVRA VDYHRMDGAE 1900
    PTCSLGFWVY QPLLNPFSTT AVSTCWVSVE TKPGGDFDFC LLRPPGQQME 1950
    NGVSPEGLLP RRLGYSRGNR VGGLVVGMVL VAEHKQVNRH FNSNSVTFGW 2000
    STAPVNPMAA EIVTNQAHST SRHAWLSIGA QNKGPLFPGI PNHFPDSCAS 2050
    TIVGAMDTSL GGRPSTGVCG PAISFQNNGD VYENDTPSVM FATYDPLTSG 2100
    TGVALTNSIN PASLALVRIS NNDFDTSGFA NDKNVVVQMS WEMYTGTNQI 2150
    RGQVTPMSGT NYTFTSTGAN TLVLWQERML SYDGHQAILY SSQLERTAEY 2200
    FQNDIVNIPE NSMAVFNVET NSASFQIGIR PDGYMVTGGS IGINVPLEPE 2250
    TRFQYVGILP LSAALSGPSG NMGRARRVFQ 2280

    Note: Produced from the genomic RNA.

    Length:2,280
    Mass (Da):251,007
    Last modified:October 11, 2004 - v1
    Checksum:i2402A87ACE40873F
    GO
    Isoform Subgenomic capsid protein (identifier: Q672I1-2) [UniParc]FASTAAdd to Basket

    Also known as: VP1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1719: Missing.

    Note: Produced from the subgenomic RNA.

    Show »
    Length:561
    Mass (Da):60,113
    Checksum:i5B6C8A3ED75E4E3C
    GO
    Isoform Uncharacterized protein VP3 (identifier: Q672I0-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q672I0.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative initiation from the subgenomic RNA.

    Length:161
    Mass (Da):17,553
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 17191719Missing in isoform Subgenomic capsid protein. CuratedVSP_034391Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY694184 Genomic RNA. Translation: AAU09265.2.
    RefSeqiYP_077278.1. NC_006269.1. [Q672I1-1]

    Genome annotation databases

    GeneIDi5176815.

    Keywords - Coding sequence diversityi

    Alternative initiation, Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY694184 Genomic RNA. Translation: AAU09265.2 .
    RefSeqi YP_077278.1. NC_006269.1. [Q672I1-1 ]

    3D structure databases

    ProteinModelPortali Q672I1.
    SMRi Q672I1. Positions 1207-1705.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C24.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5176815.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR001665. Norovirus_pept_C37.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF05416. Peptidase_C37. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural and functional characterization of sapovirus RNA-dependent RNA polymerase."
      Fullerton S.W., Blaschke M., Coutard B., Gebhardt J., Gorbalenya A., Canard B., Tucker P.A., Rohayem J.
      J. Virol. 81:1858-1871(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPOLG_SVSAP
    AccessioniPrimary (citable) accession number: Q672I1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3