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Protein

Mannose-6-phosphate isomerase

Gene

pmi1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (pmi1)
  2. Phosphomannomutase (AFUA_6G06580)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108ZincBy similarity1
Metal bindingi110ZincBy similarity1
Metal bindingi135ZincBy similarity1
Metal bindingi292ZincBy similarity1
Active sitei311By similarity1

GO - Molecular functioni

  • mannose-6-phosphate isomerase activity Source: AspGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: EnsemblFungi
  • GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  • mannose metabolic process Source: AspGD
  • protein glycosylation Source: EnsemblFungi

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi5.3.1.8 508
UniPathwayiUPA00126; UER00423

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:pmi1
Synonyms:manA
ORF Names:AfA6E3.135c, AFUA_1G13280
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu1g13280

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001942401 – 457Mannose-6-phosphate isomeraseAdd BLAST457

Proteomic databases

PRIDEiQ66WM4

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00001256

Structurei

3D structure databases

ProteinModelPortaliQ66WM4
SMRiQ66WM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000241277
InParanoidiQ66WM4
KOiK01809
OMAiMGTHPSN
OrthoDBiEOG092C3NWF

Family and domain databases

Gene3Di2.60.120.10, 4 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit
PS00966 PMI_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q66WM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPLLRLQ CGVNSYDWGK VGHESAAAKY AATTAASDFS IQSDKPYAEL
60 70 80 90 100
WMGTHPSLPS KDLETQRTLL DMVQDNQALI SQEVSERYGG KLPFLFKVLS
110 120 130 140 150
IRKALSIQAH PNKKLAEKLH ARDPRNYPDD NHKPEMTIAI TPFEGLCGFR
160 170 180 190 200
PLVEIIHFLK AVAPLRQLVG ERAASEFENT VKGSEESEDP AVTEKNKQAL
210 220 230 240 250
RTLFTSLMRS SPESIEAATK ELVAIAQNSP ETFTTSSSTP ETNPTNPAEL
260 270 280 290 300
AAITVRLNGQ FPNDIGSFVF FFLNFVKLEP GEAMFLKADD IHAYISGDII
310 320 330 340 350
ECMASSDNVV RAGFTPKFKD VDTLVDMLTY SYAPIAEQKL EPTDYPYAVL
360 370 380 390 400
NAPAYSSGSS CILYDPPIEE FSVVKTDLKR QGAKATFDGI SGPSIVICTA
410 420 430 440 450
GAGKITVGPK TEEVNEGYVF FVGANAECII ESTGEDTFTT FKAFCDLTGK

EDMVNGN
Length:457
Mass (Da):49,930
Last modified:December 6, 2005 - v2
Checksum:i27C3E5F425BAA224
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80I → V in AAU06585 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700212 mRNA Translation: AAU06585.1
BX649606 Genomic DNA Translation: CAF32047.1
AAHF01000004 Genomic DNA Translation: EAL90660.1
RefSeqiXP_752698.1, XM_747605.1

Genome annotation databases

EnsemblFungiiCADAFUAT00007084; CADAFUAP00007084; CADAFUAG00007084
GeneIDi3510576
KEGGiafm:AFUA_1G13280

Similar proteinsi

Entry informationi

Entry nameiMPI_ASPFU
AccessioniPrimary (citable) accession number: Q66WM4
Secondary accession number(s): Q4WSC2, Q6MYF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health