Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mannose-6-phosphate isomerase

Gene

pmi1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081ZincBy similarity
Metal bindingi110 – 1101ZincBy similarity
Metal bindingi135 – 1351ZincBy similarity
Metal bindingi292 – 2921ZincBy similarity
Active sitei311 – 3111By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: ASPGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell wall mannoprotein biosynthetic process Source: EnsemblFungi
  2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  3. mannose metabolic process Source: ASPGD
  4. protein glycosylation Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi5.3.1.8. 508.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:pmi1
Synonyms:manA
ORF Names:AfA6E3.135c, AFUA_1G13280
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: EnsemblFungi
  2. nucleus Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Mannose-6-phosphate isomerasePRO_0000194240Add
BLAST

Proteomic databases

PRIDEiQ66WM4.

Structurei

3D structure databases

ProteinModelPortaliQ66WM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241277.
InParanoidiQ66WM4.
KOiK01809.
OMAiNAECIIE.
OrthoDBiEOG7WDNCR.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66WM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPLLRLQ CGVNSYDWGK VGHESAAAKY AATTAASDFS IQSDKPYAEL
60 70 80 90 100
WMGTHPSLPS KDLETQRTLL DMVQDNQALI SQEVSERYGG KLPFLFKVLS
110 120 130 140 150
IRKALSIQAH PNKKLAEKLH ARDPRNYPDD NHKPEMTIAI TPFEGLCGFR
160 170 180 190 200
PLVEIIHFLK AVAPLRQLVG ERAASEFENT VKGSEESEDP AVTEKNKQAL
210 220 230 240 250
RTLFTSLMRS SPESIEAATK ELVAIAQNSP ETFTTSSSTP ETNPTNPAEL
260 270 280 290 300
AAITVRLNGQ FPNDIGSFVF FFLNFVKLEP GEAMFLKADD IHAYISGDII
310 320 330 340 350
ECMASSDNVV RAGFTPKFKD VDTLVDMLTY SYAPIAEQKL EPTDYPYAVL
360 370 380 390 400
NAPAYSSGSS CILYDPPIEE FSVVKTDLKR QGAKATFDGI SGPSIVICTA
410 420 430 440 450
GAGKITVGPK TEEVNEGYVF FVGANAECII ESTGEDTFTT FKAFCDLTGK

EDMVNGN
Length:457
Mass (Da):49,930
Last modified:December 6, 2005 - v2
Checksum:i27C3E5F425BAA224
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801I → V in AAU06585 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700212 mRNA. Translation: AAU06585.1.
BX649606 Genomic DNA. Translation: CAF32047.1.
AAHF01000004 Genomic DNA. Translation: EAL90660.1.
RefSeqiXP_752698.1. XM_747605.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00007084; CADAFUAP00007084; CADAFUAG00007084.
GeneIDi3510576.
KEGGiafm:AFUA_1G13280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700212 mRNA. Translation: AAU06585.1.
BX649606 Genomic DNA. Translation: CAF32047.1.
AAHF01000004 Genomic DNA. Translation: EAL90660.1.
RefSeqiXP_752698.1. XM_747605.1.

3D structure databases

ProteinModelPortaliQ66WM4.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ66WM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00007084; CADAFUAP00007084; CADAFUAG00007084.
GeneIDi3510576.
KEGGiafm:AFUA_1G13280.

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241277.
InParanoidiQ66WM4.
KOiK01809.
OMAiNAECIIE.
OrthoDBiEOG7WDNCR.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
BRENDAi5.3.1.8. 508.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Aspergillus fumigatus strain YJ-407 phosphomannose isomerase (pmi) mRNA."
    Yu X., Jin C.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: YJ-407.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  3. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiMPI_ASPFU
AccessioniPrimary (citable) accession number: Q66WM4
Secondary accession number(s): Q4WSC2, Q6MYF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: April 1, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.