Q66WM4 (MPI_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

Name:	pmi1
Synonyms:	manA
ORF Names:	AfA6E3.135c, AFUA_1G13280

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Mannose-6-phosphate isomerase		PRO_0000194240
Sites
	Active site	311	1	By similarity
	Metal binding	108	1	Zinc By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	135	1	Zinc By similarity
	Metal binding	292	1	Zinc By similarity
Experimental info
	Sequence conflict	80	1	I → V in AAU06585. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q66WM4 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 27C3E5F425BAA224	FASTA	457	49,930
	10 20 30 40 50 60 MPPVPLLRLQ CGVNSYDWGK VGHESAAAKY AATTAASDFS IQSDKPYAEL WMGTHPSLPS 70 80 90 100 110 120 KDLETQRTLL DMVQDNQALI SQEVSERYGG KLPFLFKVLS IRKALSIQAH PNKKLAEKLH 130 140 150 160 170 180 ARDPRNYPDD NHKPEMTIAI TPFEGLCGFR PLVEIIHFLK AVAPLRQLVG ERAASEFENT 190 200 210 220 230 240 VKGSEESEDP AVTEKNKQAL RTLFTSLMRS SPESIEAATK ELVAIAQNSP ETFTTSSSTP 250 260 270 280 290 300 ETNPTNPAEL AAITVRLNGQ FPNDIGSFVF FFLNFVKLEP GEAMFLKADD IHAYISGDII 310 320 330 340 350 360 ECMASSDNVV RAGFTPKFKD VDTLVDMLTY SYAPIAEQKL EPTDYPYAVL NAPAYSSGSS 370 380 390 400 410 420 CILYDPPIEE FSVVKTDLKR QGAKATFDGI SGPSIVICTA GAGKITVGPK TEEVNEGYVF 430 440 450 FVGANAECII ESTGEDTFTT FKAFCDLTGK EDMVNGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aspergillus fumigatus strain YJ-407 phosphomannose isomerase (pmi) mRNA." Yu X., Jin C. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: YJ-407.
[2]	"Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster." Pain A., Woodward J.R., Quail M.A., Anderson M.J., Clark R., Collins M., Fosker N., Fraser A., Harris D.E., Larke N., Murphy L.D., Humphray S., O'Neil S., Pertea M., Price C., Rabbinowitsch E., Rajandream M.A., Salzberg S.L. , Saunders D., Seeger K., Sharp S., Warren T., Denning D.W., Barrell B.G., Hall N. Fungal Genet. Biol. 41:443-453(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[3]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY700212 mRNA. Translation: AAU06585.1. BX649606 Genomic DNA. Translation: CAF32047.1. AAHF01000004 Genomic DNA. Translation: EAL90660.1.
RefSeq	XP_752698.1. XM_747605.1.
3D structure databases
ProteinModelPortal	Q66WM4.
ModBase	Search...
Proteomic databases
PRIDE	Q66WM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00007084; CADAFUAP00007084; CADAFUAG00007084.
GeneID	3510576.
KEGG	afm:AFUA_1G13280.
Phylogenomic databases
eggNOG	COG1482.
HOGENOM	HOG000241277.
KO	K01809.
OMA	EMTIAIT.
OrthoDB	EOG4TF3V5.
Enzyme and pathway databases
UniPathway	UPA00126; UER00423.
Family and domain databases
Gene3D	2.60.120.10. 3 hits.
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_ASPFU

Accession

Primary (citable) accession number: Q66WM4
Secondary accession number(s): Q4WSC2, Q6MYF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program