Q66T60 (L_RABVB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large structural protein
Short name=Protein L

Alternative name(s):
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Rabies virus (strain silver-haired bat-associated) (RABV) (SHBRV) [Complete proteome]

Taxonomic identifier

445793 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus › Rabies virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	2128 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyltransferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyltransferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviruses protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2128	2128	Large structural protein		PRO_0000294418
Regions
	Domain	612 – 800	189	RdRp catalytic
	Region	1563 – 2128	566	Interaction with P protein By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66T60 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A039BB2078B76EFD	FASTA	2,128	243,090
	10 20 30 40 50 60 MMIDPGEVYD DPIDLIESEA EPKGNPTIPN ILRNSDYNLN SPLIEDPARL MLEWLKTGNR 70 80 90 100 110 120 PLRMTLTDNC SRSHKVLKDY FKRVDLGSLK VGGAAAQSMI SLWLYGAHSE SNRSRKCIND 130 140 150 160 170 180 LAQFYYKSSP IEKLLNCTLG NRGLKTPPEG VLSCLARVDY DKAFGRYLAN IYSSYLFFHV 190 200 210 220 230 240 ITLYMNALDW DEEKTILALW RDITSIDTGK DLVKFKDQIW GLLIVTKDFV YSQSTGCLFD 250 260 270 280 290 300 RNYTLMLKDL FLSRFNSLMI LLSPPEPRYS DDLISQLCQL YIAGDQVLSM CGNSGYEVIK 310 320 330 340 350 360 ILEPYVVNSL VQRAEKFRPL IHSLGDFPTF IRDKVGQLEG TFGPSAKRFF KVLDQFDNIH 370 380 390 400 410 420 DLVFVYGCYR HWGHPYIDYR KGLSKLYDQV HLKKVIDKSY QECLASDLAR RILRWGFDKY 430 440 450 460 470 480 SKWYLDSRLL SRDHPLIPYI KTQTWPPRHI VDLVGDTWHK LPITQIFEIP ESMDPSEILD 490 500 510 520 530 540 DKSHSFTRTR LASWLSENRG GPVPSEKVII TALSKPPVNP REFLKSIDLG GLPDEDLIIG 550 560 570 580 590 600 LKPKERELKI EGRFFALMSW NLRLYFVITE KLLANYILPL FDALTMTDNL NKVFKKLIDR 610 620 630 640 650 660 VTGQGLLDYS RVTYAFRLDY EKWNNHQRLE STEDVFSVLD QVFGLKRVFS RTHEFFQRSW 670 680 690 700 710 720 IYYSDRSDLI GLWEDQIYCL DMSDGPTCWN GQDGGLEGLR QKGWSLVSLL MIDRESQTRN 730 740 750 760 770 780 TRTKILAQGD NQVLCPTYML SPGLSREGLL YELESISRNA LSIYRAIEEG ASKLGLIIKK 790 800 810 820 830 840 EETMCSYDFL IYGKTPLFRG NILVPESKRW ARVSCISNDQ IVNLANTMST VSTNALTVAQ 850 860 870 880 890 900 HSQSLIKPMR DFLLMSVQAV FHYLLFSPIL KGRVYKILSA EGDNFLLAMS RIIYLDPSLG 910 920 930 940 950 960 GVSGMSLGRF HIRQFSDPVS EGLSFWREIW LSSNESWIHA LCQEAGNPDL GERTLESFTR 970 980 990 1000 1010 1020 LLEDPTTLNI KGGASPTILL KDAIRKALYD EVDEVENSEF REAILLSKTH RDNFILFLKS 1030 1040 1050 1060 1070 1080 IEPLFPRFLS ELFSSSFLGI PESIIGLIQN SRTIRRQFRR SLSRTLEESF YNSETHGINR 1090 1100 1110 1120 1130 1140 MTQTPQRVGR VWPCSSERAD LLREISWGRK VVGTTVPHPS EMLGLIPKSS ISCTCGAAGG 1150 1160 1170 1180 1190 1200 GNPRISVSVL PSFDQSFFSR GPLKGYLGSS TSMSTQLFHA WEKVTNVHVV KRALSLKESI 1210 1220 1230 1240 1250 1260 NWFITRNSNR AQTLIRNIMS LTGPDFPLEE APVFKRTGSA LHRFKSARYS EGGYSSVCPN 1270 1280 1290 1300 1310 1320 LLSHISVSTD TMSDLTQDGR NYDFMFQPLM LYAQTWTSEL VQKDTRLKDS TFHWHLRCNK 1330 1340 1350 1360 1370 1380 CIRPIDDMTL DTSQVFEFPD VSRRISRMVS GAVPHFRKLP DIRLRPGDFE SLSGKEKSRH 1390 1400 1410 1420 1430 1440 IGSAQGLLYS ILVAIHDSGY NDGTIFPVNI YSKVSPRDYL RGLARGVLIG SSICFLTRMT 1450 1460 1470 1480 1490 1500 NININRPLEL ISGVISYILL RLDNHPSLYI MLREPSLRGE IFSIPQKVPA AYPTTMKEGN 1510 1520 1530 1540 1550 1560 RSILCYLQHV LRYEREAITA SPENDWLWIF SDFRSSKMTY LTLITYQSHL LLQRVDKNLS 1570 1580 1590 1600 1610 1620 KSMRANLRQM SSLMRQVLGG HGEDTLESDE DIQRLLKDSL RRTRWVDQEV RHAARTMTGS 1630 1640 1650 1660 1670 1680 YSPHRRVSRK AGCSEWVCSA QQVAVSTSAN PAPASELDIR TLSRRLQNPL ISGLRVVQWA 1690 1700 1710 1720 1730 1740 TGAHYKLKPI LDDLNVFPSL CLVVGDGSGG ISRAVLNMFP DARLVFNSLL EVNDLMASGT 1750 1760 1770 1780 1790 1800 HPLPPSAIMS GGDDIISRVI DFDSIWEKPS DLRNLTTWRY FQSVQEQVNM SYDLIICDAE 1810 1820 1830 1840 1850 1860 VTDIASINRI TLLMSDFALS IDGPLYLVFK TYGTMLVNPD YRAIQHLSRA FPAVTGFITQ 1870 1880 1890 1900 1910 1920 MTSSFSSELY LRFSKRGKFF RDAEYLTSST LREMSLVLFN CSSPKSEMQR ARSLNYQDLV 1930 1940 1950 1960 1970 1980 RGFPDEVISN PYNEMIITLI DSDVESFLVH KMVDDLELQR GTLSKVSIII AIMIVFSNRV 1990 2000 2010 2020 2030 2040 FNVSKPLTDP LFYPPFDPKI LRHFNICCST MMYLSTALGD VPSFARLHDL YNRPITYYFR 2050 2060 2070 2080 2090 2100 KQVIRGNIYL SWSWSDDTSV FKRVACNSSL SLSSHWIRLI YKIVKTTRLV GSIEDLSGEI 2110 2120 EKHLRGYNRW ITLDDIRSRS SLLDYSCL

References

[1]

"Identification of viral genomic elements responsible for rabies virus neuroinvasiveness." Faber M., Pulmanausahakul R., Nagao K., Prosniak M., Rice A.B., Koprowski H., Schnell M.J., Dietzschold B. Proc. Natl. Acad. Sci. U.S.A. 101:16328-16332(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY705373 Genomic RNA. Translation: AAU11519.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR026890. Mononeg_mRNAcap. IPR014023. Mononeg_RNA_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. IPR025786. RNA-direct_RNA_pol_L. [Graphical view]
Pfam	PF14318. Mononeg_mRNAcap. 1 hit. PF00946. Mononeg_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
TIGRFAMs	TIGR04198. paramyx_RNAcap. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_RABVB

Accession

Primary (citable) accession number: Q66T60

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program