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Protein

Pleckstrin homology domain-containing family G member 5

Gene

Plekhg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that activates RHOA and maybe the NF-kappa-B signaling pathway. Involved in the control of neuronal cell differentiation. Plays a role in angiogenesis through regulation of endothelial cells chemotaxis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • endothelial cell chemotaxis Source: UniProtKB
  • endothelial cell migration Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • regulation of Rho protein signal transduction Source: InterPro

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family G member 5
Short name:
PH domain-containing family G member 5
Alternative name(s):
Synectin-binding RhoA exchange factor
Short name:
SYX
Gene namesi
Name:Plekhg5
Synonyms:Kiaa0720, Syx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2652860. Plekhg5.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • endocytic vesicle Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MGI

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Animals develop normally and show no clear neurologic symptoms as adults. However, electrophysiologic studies indicated that mutant mice have decreased motor nerve conduction velocities and delayed compound action potentials. Mutant mice perform slightly less well than control mice in the rotarod test. Detailed studies of the motor neurons of the ventral horn and of neuromuscular junctions do not show apparent abnormalities in mutant mice up to 1 year of age, indicating that they do not develop detectable signs of lower motor neuron disease.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071351 – 1073Pleckstrin homology domain-containing family G member 5Add BLAST1073

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei793PhosphothreonineCombined sources1
Modified residuei798PhosphoserineCombined sources1
Modified residuei909PhosphothreonineCombined sources1
Modified residuei911PhosphoserineCombined sources1
Modified residuei936PhosphoserineCombined sources1
Modified residuei941PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ66T02.
PRIDEiQ66T02.

PTM databases

iPTMnetiQ66T02.
PhosphoSitePlusiQ66T02.

Expressioni

Tissue specificityi

Expressed in neurons and glial cells of the peripheral nervous system, with highest levels of expression in the brain and sciatic nerve endoneurium. Isoform 2 is expressed at detectable levels only in malignant cells.2 Publications

Developmental stagei

Regulated during development, with the highest level at postnatal days 10 to 14, suggesting a role in myelination of the peripheral nervous system.1 Publication

Gene expression databases

BgeeiENSMUSG00000039713.
ExpressionAtlasiQ66T02. baseline and differential.
GenevisibleiQ66T02. MM.

Interactioni

Subunit structurei

Interacts with GIPC1/synectin and RHOA.By similarity

Protein-protein interaction databases

BioGridi234677. 13 interactors.
ELMiQ66T02.
IntActiQ66T02. 3 interactors.
STRINGi10090.ENSMUSP00000081132.

Structurei

3D structure databases

ProteinModelPortaliQ66T02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini406 – 598DHPROSITE-ProRule annotationAdd BLAST193
Domaini654 – 754PHPROSITE-ProRule annotationAdd BLAST101

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi107 – 111Poly-Glu5
Compositional biasi369 – 373Poly-Glu5
Compositional biasi778 – 790Poly-GluAdd BLAST13

Phylogenomic databases

eggNOGiKOG3521. Eukaryota.
ENOG410XRPT. LUCA.
GeneTreeiENSGT00510000046843.
HOVERGENiHBG058106.
InParanoidiQ66T02.
KOiK19464.
OMAiGRSCSMD.
OrthoDBiEOG091G00RK.
PhylomeDBiQ66T02.
TreeFamiTF316755.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR029071. Ubiquitin-rel_dom.
PfamiView protein in Pfam
PF00621. RhoGEF. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q66T02-1) [UniParc]FASTAAdd to basket
Also known as: SYX1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTGPGVSGR RAAARPSSEL PSPDSQLLWV GGHAHSSDSQ VCHHADCQQL
60 70 80 90 100
HHRGPLNLCE TCDSKFHSTL HYDGHVRFDL PPQGSVLARN VSTRSCPPRT
110 120 130 140 150
SPAADLEEEE EGCTDGKGDR KSAGLKISKK KARRRHTDDP SKECFTLKFD
160 170 180 190 200
LNVDIETEIV PAMKKKSLGE VLLPVFERKG IALGKVDIYL DQSNTPLSLT
210 220 230 240 250
FEAYRFGGHY LRVKAKPGDE GKVEQGVKDS KSLSLPALRP SGAGPPVSER
260 270 280 290 300
VDPQSRRESS LDILAPGRRR KNMSEFLGEA GIPGHEPPAP SSCSLPVGSS
310 320 330 340 350
GGTSSGINES WKNRAASRFS GFFSSSPSTS AFSREVDKME QLESKLHAYS
360 370 380 390 400
LFGLPRMPRR LRFDHDSWEE EEEDDEEDEE SSGLRLEDSW RELTDGHEKL
410 420 430 440 450
TRRQCHQQEA VWELLHTEVS YIRKLRVITN LFLCCLLNLQ ESGLLCEVEA
460 470 480 490 500
ERLFSNIPEI AKLHRGLWGS VMVPVLEKAR RTRALLQPSD FLKGFKMFGS
510 520 530 540 550
LFKPYIRYCM EEEGCMEYMR GLLRDNDLFR AYVTWAEKHQ QCQRLKLSDM
560 570 580 590 600
LAKPHQRLTK YPLLLKSVLR KTDDPRTKEA IVTMISSVER FIHHVNTCMR
610 620 630 640 650
QRQERQRLAG VVSRIDAYEV VEGSNDEVDK LLKEFLHLDL TAPMPGTSPE
660 670 680 690 700
ETRQLLLEGS LRMKEGKDSK MDVYCFLFTD LLLVTKAVKK AERTKVIRPP
710 720 730 740 750
LLVDKIVCRE LRDPGSFLLI YLNEFHSAVG AYTFQASSQA LCRSWVDTIY
760 770 780 790 800
NAQNQLQQLR AQLSAQEHPG SQHLQSLEEE EDEQEEEGEE SGTSAASSPT
810 820 830 840 850
ILRKSSNSLD SEHCTSDGST ETLAMVVVEP GATLSSPEFE GGPVSSQSDE
860 870 880 890 900
SSLSNTASSV TPTSELLPLG PVDGRSCSMD SAYGTLSPTS LQDFVAPHPV
910 920 930 940 950
VEPAPVPQTP SPQPSPRLRR RTPVQLLPRP PRLLKSKSEA SLLQLLSGTP
960 970 980 990 1000
AARGVPPAPS RSLSELCLIS VAPGVRTQRP LQEGGPGWNG PGMCDPCHGP
1010 1020 1030 1040 1050
QLSESENRPS HMTGGPADSA RRRCREMPSG TMSRVQSEPP SGVSAQHRKL
1060 1070
TLAQLYRIRT TLLLNSTLTA SEV
Length:1,073
Mass (Da):118,924
Last modified:October 11, 2004 - v1
Checksum:iE34A0B88D61CEE2B
GO
Isoform 2 (identifier: Q66T02-2) [UniParc]FASTAAdd to basket
Also known as: SYX2

The sequence of this isoform differs from the canonical sequence as follows:
     1072-1073: Missing.

Note: No experimental confirmation available.
Show »
Length:1,071
Mass (Da):118,696
Checksum:i9B88D61CEE2BFEDA
GO

Sequence cautioni

The sequence CAM24581 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0285871072 – 1073Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY605057 mRNA. Translation: AAU04953.1.
AY605058 mRNA. Translation: AAU04954.1.
AL772240, AL611927 Genomic DNA. Translation: CAM19697.1.
AL772240, AL611927 Genomic DNA. Translation: CAM19699.2.
AL611927, AL772240 Genomic DNA. Translation: CAM24581.1. Sequence problems.
AL611927, AL772240 Genomic DNA. Translation: CAM24582.2.
AK129198 mRNA. Translation: BAC98008.1.
BC023181 mRNA. Translation: AAH23181.1.
BC064091 mRNA. Translation: AAH64091.1.
CCDSiCCDS18987.2. [Q66T02-1]
RefSeqiNP_001272928.1. NM_001285999.1. [Q66T02-1]
UniGeneiMm.332102.
Mm.486442.

Genome annotation databases

EnsembliENSMUST00000084115; ENSMUSP00000081132; ENSMUSG00000039713. [Q66T02-2]
ENSMUST00000105661; ENSMUSP00000101286; ENSMUSG00000039713. [Q66T02-1]
GeneIDi269608.
KEGGimmu:269608.
UCSCiuc008vzh.2. mouse. [Q66T02-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPKHG5_MOUSE
AccessioniPrimary (citable) accession number: Q66T02
Secondary accession number(s): A2A8B6
, A2A8B7, Q66T00, Q6P3B1, Q6ZQ62, Q8R571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 11, 2004
Last modified: August 30, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot