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Protein

LARGE xylosyl- and glucuronyltransferase 2

Gene

LARGE2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 Mn2+ ions per subunit. The xylosyltransferase part binds one Mn2+ and the beta-1,3-glucuronyltransferase part binds one Mn2+.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi225Manganese 1By similarity1
Metal bindingi227Manganese 1By similarity1
Metal bindingi546Manganese 2By similarity1
Metal bindingi548Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
LARGE xylosyl- and glucuronyltransferase 2By similarity (EC:2.4.-.-)
Alternative name(s):
Glycosyltransferase-like 1B
Including the following 2 domains:
Xylosyltransferase LARGE2Curated (EC:2.4.2.-)
Beta-1,3-glucuronyltransferase LARGE2Curated (EC:2.4.1.-)
Gene namesi
Name:LARGE2By similarity
Synonyms:GYLTL1B
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 739LumenalSequence analysisAdd BLAST708

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268141 – 739LARGE xylosyl- and glucuronyltransferase 2Add BLAST739

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ66PG4.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000037568.

Structurei

3D structure databases

ProteinModelPortaliQ66PG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 396Xylosyltransferase activityBy similarityAdd BLAST276
Regioni397 – 739Glucuronyltransferase activityBy similarityAdd BLAST343

Sequence similaritiesi

In the C-terminal section; belongs to the glycosyltransferase 49 family.Curated
In the N-terminal section; belongs to the glycosyltransferase 8 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ66PG4.
KOiK09668.
PhylomeDBiQ66PG4.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66PG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCSWRVKLK LLLATITLAV LLSWLYLFVG SLEYGRFLLL PPCLGEQPSR
60 70 80 90 100
DVEREALASQ VRRVEEENQQ LRMQLGQVQA EGSDGNPQWA ASAEDGPPLG
110 120 130 140 150
GERNNRTACP EQRMVRKCEL LHVAIVCAGH NASRDVVTLV KSILFHRKNP
160 170 180 190 200
LHFHFITDSV AHQILQTLFQ SWMVPSIHVS FYNADDLKPE VSWIPNKHYS
210 220 230 240 250
GIYGLMKLTL TKALPSNLSK VIVLDTDITF ATDIAELWAV FGKFSEKQVI
260 270 280 290 300
GLVENQSDWY LGNLWKNHKP WPALGRGFNT GVILLLLDRL RRLGWEQMWR
310 320 330 340 350
LTAERELMSM LSTSLADQDI FNAVIKQNPA LVYRLPCFWN VQLSDHTRSE
360 370 380 390 400
LCYTEVSDLK VIHWNSPKKL RVKNKHVEFF RNLYLTFLEY DGNLLRRELF
410 420 430 440 450
GCASLPSPPS DQLQQALEEL DEDDPCYDFR RQHLTQHRVH LFFLQYEFLA
460 470 480 490 500
LPNPTDVTLV AQLSMDRLQM LEAICKHWAG PISLALYMSD AEAQQFLRYA
510 520 530 540 550
QASEVLSARR NVAYHIVYKE GQFYPINLLR NVALANTQTP YVFLTDIDFL
560 570 580 590 600
PMYGLYDYLR NSIQQLELPH RKAALIVPAF ETLHYRLTFP KSKAELLSML
610 620 630 640 650
DMGSLYTFRY HVWPKGHAPT DYAKWRTATV PYRVAWQPDF EPYVVVRRDC
660 670 680 690 700
PKYDQRFVGF GWNKVSHIME LDAQEYELLV LPNAFMIHMP HAPSFDISKF
710 720 730
RLSAGYRGCL QTLREEFHQD LSRKYGAAAL KYLTAERNL
Length:739
Mass (Da):85,568
Last modified:October 11, 2004 - v1
Checksum:iCF65F7C0E65414AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY662336 mRNA. Translation: AAU12249.1.
RefSeqiNP_001004404.1. NM_001004404.2.
UniGeneiGga.15229.
Gga.20282.

Genome annotation databases

GeneIDi423200.
KEGGigga:423200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY662336 mRNA. Translation: AAU12249.1.
RefSeqiNP_001004404.1. NM_001004404.2.
UniGeneiGga.15229.
Gga.20282.

3D structure databases

ProteinModelPortaliQ66PG4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000037568.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbiQ66PG4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi423200.
KEGGigga:423200.

Organism-specific databases

CTDi228366.

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ66PG4.
KOiK09668.
PhylomeDBiQ66PG4.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ66PG4.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLARG2_CHICK
AccessioniPrimary (citable) accession number: Q66PG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.