Q66PG3 (LARGE_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosyltransferase-like protein LARGE1
EC=2.4.-.-

Alternative name(s):
Acetylglucosaminyltransferase-like 1A

Gene names

Name:	LARGE
Synonyms:	LARGE1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase which participates in glycosylation of alpha-dystroglycan. May carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. May be involved in the addition of a repeated disaccharide unit By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	Glycosyltransferase-like protein LARGE1		PRO_0000226809
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 756	725	Lumenal Potential
	Coiled coil	55 – 90	36	Potential
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q66PG3 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: BC9DC75B9349C92E	FASTA	756	88,046
	10 20 30 40 50 60 MLGICRGRRK FLAASLTVLF VPAVTWIYLF AGSFEDGKPV SLSPLESQPH SPRYTASSQR 70 80 90 100 110 120 DRESLEVRMR EVEEENRVLR KQLSLAQGRS PSHHRGNHSK TYSMEEGTGD SESLRAGIVA 130 140 150 160 170 180 GNSSECGQQP AVEKCETIHV AIVCAGYNAS RDVVTLVKSV LFHRRNPLHF HLIADAIAKQ 190 200 210 220 230 240 ILATLFQTWM VPAVRIDFYD ADELKSEVSW IPNKHYSGIY GLMKLVLTKT LPSNLERVIV 250 260 270 280 290 300 LDTDITFATD IAELWAVFHK FKGQQVLGLV ENQSDWYLGN LWKNHRPWPA LGRGYNTGVI 310 320 330 340 350 360 LLLLDKLRKM KWEQMWRLTA ERELMSMLST SLADQDIFNA VIKQNPFLVY QLPCFWNVQL 370 380 390 400 410 420 SDHTRSEQCY RDVSDLKVIH WNSPKKLRVK NKHVEFFRNL YLTFLEYDGN LLRRELFGCP 430 440 450 460 470 480 SEADVNSENL QKQLSEPDED DLCYEFRRER FTVHRTHLYF LHYEYEPASD NTDVTLVAQL 490 500 510 520 530 540 SMDRLQMLEA ICKHWEDPIS LALYLSDAEA QQFLRYAQGS EVLMSRHNVG YHIVYKEGQF 550 560 570 580 590 600 YPVNLLRNVA MKHISTPYMF LSDIDFLPMY GLYEYLRKSV TQLDLANTKK ALIIPAFETL 610 620 630 640 650 660 RYRLSFPKSK AELLSMLDMG TLFTFRYHVW TKGHAPTNFA KWRTATTPYR VEWEADFEPY 670 680 690 700 710 720 VVVRKDCPEY DRRFVGFGWN KVAHIMELDA QEYEFTVLPN AYMIHMPHAP SFDITKFRSN 730 740 750 KQYRICLKTL KEEFQQDMSR HYGFAALKYL TAENNS

References

[1]	"Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies." Grewal P.K., McLaughlan J.M., Moore C.J., Browning C.A., Hewitt J.E. Glycobiology 15:912-923(2005) [PubMed: 15958417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY662337 mRNA. Translation: AAU12250.1.
IPI	IPI00594194.
RefSeq	NP_001004383.1. NM_001004383.1.
UniGene	Gga.22653.
3D structure databases
ProteinModelPortal	Q66PG3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66PG3.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49. GT8. Glycosyltransferase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	418062.
KEGG	gga:418062.
Organism-specific databases
CTD	9215.
Phylogenomic databases
eggNOG	veNOG04906.
GeneTree	ENSGT00530000063165.
HOGENOM	HBG387493.
HOVERGEN	HBG052308.
InParanoid	Q66PG3.
OrthoDB	EOG4KKZ2K.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
KO	K09668.
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LARGE_CHICK

Accession

Primary (citable) accession number: Q66PG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program