Reviewed, UniProtKB/Swiss-Prot Q66PG2 (LARGE_DANRE)

Names and origin

Protein names

Recommended name:
    Glycosyltransferase-like protein LARGE1
    EC=2.4.-.-
Alternative name(s):
    Acetylglucosaminyltransferase-like 1A

Gene names

Name:	large
Synonyms:	large1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase which participates in glycosylation of alpha-dystroglycan. May carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. May be involved in the addition of a repeated disaccharide unit By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	Glycosyltransferase-like protein LARGE1		PRO_0000226810
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 757	726	Lumenal Potential
	Coiled coil	50 – 82	33	Potential
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	273	1	N-linked (GlcNAc...) Potential
	Glycosylation	738	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q66PG2-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 88AB8E3E59172775	FASTA	757	87,673
	10 20 30 40 50 60 MLGMCRGRRK FVAASLALIF IPALTWLYLS SANITVKPLP LSPLDPQSSA VVGAAAEHQS 70 80 90 100 110 120 LELRLRDVEE HNNALRREIS RTPRVPTHSS HPSSSRHGNQ LHTHSTEEGT GDSEAKKGAA 130 140 150 160 170 180 AGNSSDCVPQ PVVKKCETIH IAIVCAGYNA SRDVVTLVKS VLFHRRNPLH FHIITDSIAR 190 200 210 220 230 240 KILADLFHTW MVPAVHVNFY DADELKSEVS WIPNKHYSGI HGLMKLVLTK TLPSDLEKVI 250 260 270 280 290 300 VLDTDITFAT DIAELWVVFH KFKGQQVLGL VENQSDWYLG NLWKNHRPWP ALGRGFNTGV 310 320 330 340 350 360 ILLLLDRLRK LKWEQMWRLT AERELMSMLS TSLADQDIFN AVIKQNPFLV HQLPCYWNVQ 370 380 390 400 410 420 LSDHTRSEKC YKDVSDLKVI HWNSPKKLRV KNKHVEFFRN LYLTFLEYDG NLLRRELFGC 430 440 450 460 470 480 PSETDHNSEN LQKTLSELDE DDPCYEFRRE RFTVHRTHVY FLHYEYEPTV DNTDVTLVAQ 490 500 510 520 530 540 LSMDRLQMLE AICKHWEGPI SLALYLSDAE AQQFLRYAQG SEVLMSRSNV GYHIVYKEGQ 550 560 570 580 590 600 FYPVNLLRNV AMGQVNTPYM FLSDIDFLPM YGLYEYLRKS VVQLDMGNTK KALVVPAFET 610 620 630 640 650 660 LRYRLSFPKS KAELLSQLDM GTLFTFRYHV WTKGHAPTDF AKWRTATTPY RVQWEADFEP 670 680 690 700 710 720 YVMVRRESPE YDRRFVGFGW NKVAHIMELD AQEYEFVVLP NAYMIHMPHA PSFDITKFRS 730 740 750 NKQYRACLKT LKEEFQQNMS RRYGFAALKY MTVDNNS

References

[1]	"Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies." Grewal P.K., McLaughlan J.M., Moore C.J., Browning C.A., Hewitt J.E. Glycobiology 15:912-923(2005) [PubMed: 15958417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY662338 mRNA. Translation: AAU12251.1.
IPI	IPI00503872.
RefSeq	NP_001004537.1.
UniGene	Dr.91325
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q66PG2.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49. GT8. Glycosyltransferase Family 8.
Genome annotation databases
Ensembl	ENSDART00000023917; ENSDARP00000013130; ENSDARG00000005126; Danio rerio. [Genome view]
GeneID	446213.
KEGG	dre:446213.
Organism-specific databases
CTD	446213.
ZFIN	ZDB-GENE-061204-1. large.
Phylogenomic databases
HOGENOM	HBG387493.
HOVERGEN	Q66PG2.
InParanoid	Q66PG2.
Gene expression databases
Bgee	Q66PG2.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LARGE_DANRE

Accession

Primary (citable) accession number: Q66PG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	December 15, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project