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Protein

Glycosyltransferase-like protein LARGE1

Gene

large

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 Mn2+ ions per subunit. The xylosyltransferase part binds one Mn2+ and the beta-1,3-glucuronyltransferase part binds one Mn2+.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi243 – 2431Manganese 1By similarity
Metal bindingi245 – 2451Manganese 1By similarity
Metal bindingi564 – 5641Manganese 2By similarity
Metal bindingi566 – 5661Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycosyltransferase-like protein LARGE1 (EC:2.4.-.-)
Alternative name(s):
Acetylglucosaminyltransferase-like 1A
Including the following 2 domains:
Xylosyltransferase LARGECurated (EC:2.4.2.-By similarity)
Beta-1,3-glucuronyltransferase LARGECurated (EC:2.4.1.-By similarity)
Gene namesi
Name:large
Synonyms:large1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-061204-1. large.

Subcellular locationi

  • Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini32 – 757726LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Glycosyltransferase-like protein LARGE1PRO_0000226810Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis
Glycosylationi738 – 7381N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ66PG2.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013130.

Structurei

3D structure databases

ProteinModelPortaliQ66PG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 414276Xylosyltransferase activityBy similarityAdd
BLAST
Regioni415 – 757343Glucuronyltransferase activityBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili50 – 8233Sequence analysisAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the glycosyltransferase 49 family.Curated
In the N-terminal section; belongs to the glycosyltransferase 8 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ66PG2.
KOiK09668.
PhylomeDBiQ66PG2.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66PG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGMCRGRRK FVAASLALIF IPALTWLYLS SANITVKPLP LSPLDPQSSA
60 70 80 90 100
VVGAAAEHQS LELRLRDVEE HNNALRREIS RTPRVPTHSS HPSSSRHGNQ
110 120 130 140 150
LHTHSTEEGT GDSEAKKGAA AGNSSDCVPQ PVVKKCETIH IAIVCAGYNA
160 170 180 190 200
SRDVVTLVKS VLFHRRNPLH FHIITDSIAR KILADLFHTW MVPAVHVNFY
210 220 230 240 250
DADELKSEVS WIPNKHYSGI HGLMKLVLTK TLPSDLEKVI VLDTDITFAT
260 270 280 290 300
DIAELWVVFH KFKGQQVLGL VENQSDWYLG NLWKNHRPWP ALGRGFNTGV
310 320 330 340 350
ILLLLDRLRK LKWEQMWRLT AERELMSMLS TSLADQDIFN AVIKQNPFLV
360 370 380 390 400
HQLPCYWNVQ LSDHTRSEKC YKDVSDLKVI HWNSPKKLRV KNKHVEFFRN
410 420 430 440 450
LYLTFLEYDG NLLRRELFGC PSETDHNSEN LQKTLSELDE DDPCYEFRRE
460 470 480 490 500
RFTVHRTHVY FLHYEYEPTV DNTDVTLVAQ LSMDRLQMLE AICKHWEGPI
510 520 530 540 550
SLALYLSDAE AQQFLRYAQG SEVLMSRSNV GYHIVYKEGQ FYPVNLLRNV
560 570 580 590 600
AMGQVNTPYM FLSDIDFLPM YGLYEYLRKS VVQLDMGNTK KALVVPAFET
610 620 630 640 650
LRYRLSFPKS KAELLSQLDM GTLFTFRYHV WTKGHAPTDF AKWRTATTPY
660 670 680 690 700
RVQWEADFEP YVMVRRESPE YDRRFVGFGW NKVAHIMELD AQEYEFVVLP
710 720 730 740 750
NAYMIHMPHA PSFDITKFRS NKQYRACLKT LKEEFQQNMS RRYGFAALKY

MTVDNNS
Length:757
Mass (Da):87,673
Last modified:October 11, 2004 - v1
Checksum:i88AB8E3E59172775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY662338 mRNA. Translation: AAU12251.1.
RefSeqiNP_001004537.1. NM_001004537.1.
UniGeneiDr.91325.

Genome annotation databases

GeneIDi446213.
KEGGidre:446213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY662338 mRNA. Translation: AAU12251.1.
RefSeqiNP_001004537.1. NM_001004537.1.
UniGeneiDr.91325.

3D structure databases

ProteinModelPortaliQ66PG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013130.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbiQ66PG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi446213.
KEGGidre:446213.

Organism-specific databases

CTDi9215.
ZFINiZDB-GENE-061204-1. large.

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ66PG2.
KOiK09668.
PhylomeDBiQ66PG2.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ66PG2.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies."
    Grewal P.K., McLaughlan J.M., Moore C.J., Browning C.A., Hewitt J.E.
    Glycobiology 15:912-923(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiLARGE_DANRE
AccessioniPrimary (citable) accession number: Q66PG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.