Q66PG1 (LARG2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosyltransferase-like protein LARGE2
EC=2.4.-.-

Alternative name(s):
Glycosyltransferase-like 1B

Gene names

Name:	gyltl1b
Synonyms:	large2
ORF Names:	si:ch211-206g24.1, si:ch211-282n12.1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase which participates in glycosylation of alpha-dystroglycan. May carry out the synthesis of glycoprotein and glycosphingolipid sugar chains By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	Glycosyltransferase-like protein LARGE2		PRO_0000226815
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 750	719	Lumenal Potential
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	87	1	A → V in CAK04442. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q66PG1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 79C9BAC9F707CBB2	FASTA	750	86,987
	10 20 30 40 50 60 MLCPCRGKLK LLVVSLSFVI LFTWLYLLVG NSENGRSLLL SACLVESTEA RLLERDVLAS 70 80 90 100 110 120 RVREVEEENR QIRLQLSQSQ GLAGQPAEGN YGNQQWVASA DTGPEDVENT AEERANHSEC 130 140 150 160 170 180 SRSPTAEKCE LLHVACVCAG HNASRDVVTL VKSILFHRRN PLHFHFITDT VANQILSTLF 190 200 210 220 230 240 QSWMVPSVQV SFYDADELKS EVSWIPNKHY SGIYGLMKLT LTKALPSNLS KVIVLDTDIT 250 260 270 280 290 300 FATDIAELWA IFRKFTEKQV IGLVENQSDW YLGNLWKNHK PWPALGRGFN TGVILLYLER 310 320 330 340 350 360 LRRMGWEQMW RLTAERELMS MLSTSLADQD IFNAFIKQNP VLVHQLPCFW NVQLSDHTRS 370 380 390 400 410 420 EQCYTEVSDL KVIHWNSPKK LRVKNKHVEF FRNLYLTFLE YDGNLLRREL FGCPSQASSE 430 440 450 460 470 480 STVLQQALEE LDEDDQCYDF RRERIMLHRV HLYFLQYEYS PTDDGTDITL VAQLSMDRLQ 490 500 510 520 530 540 MLEAICKHWE GPISLALYMS DAEAQQFLRY AQASEVLKNR KNVGYHIVYK EGQFYPVNLV 550 560 570 580 590 600 RNVALRNVNT PYVFLTDVDF LPMYGLYDYL RKSIVQLDMA NTKKALVVPA FETLRYRLSF 610 620 630 640 650 660 PKSKAELLSM LDMGTLYTFR YHVWTKGHAP TNYAKWRTAT TPYKVEWEAD FEPYVVVRRD 670 680 690 700 710 720 CPEYDQRFVG FGWNKVSHIM ELDAQEYDLI VLPNAFMIHM PHAPSFDISK FRSSPSYRYC 730 740 750 LTTLKDEFHQ DLSRKYGSAA LKYLTAQRNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies." Grewal P.K., McLaughlan J.M., Moore C.J., Browning C.A., Hewitt J.E. Glycobiology 15:912-923(2005) [PubMed: 15958417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY662339 mRNA. Translation: AAU12252.1. BX908385 Genomic DNA. Translation: CAK04901.1. BX936304 Genomic DNA. Translation: CAK04442.1.
IPI	IPI00485740.
RefSeq	NP_001004538.1. NM_001004538.1.
UniGene	Dr.36508.
3D structure databases
ProteinModelPortal	Q66PG1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66PG1.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49. GT8. Glycosyltransferase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000015786; ENSDARP00000019858; ENSDARG00000017058. ENSDART00000127953; ENSDARP00000108343; ENSDARG00000017058.
GeneID	446214.
KEGG	dre:446214.
Organism-specific databases
CTD	120071.
ZFIN	ZDB-GENE-050419-253. gyltl1b.
Phylogenomic databases
eggNOG	fiNOG13925.
GeneTree	ENSGT00530000063165.
HOGENOM	HBG387493.
HOVERGEN	HBG052308.
InParanoid	Q66PG1.
OMA	KNHKPWP.
OrthoDB	EOG45TCMQ.
PhylomeDB	Q66PG1.
Gene expression databases
Bgee	Q66PG1.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
KO	K09668.
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LARG2_DANRE

Accession

Primary (citable) accession number: Q66PG1
Secondary accession number(s): Q1LUJ7, Q1LV71

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program