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Q66LE6 (2ABD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform
Alternative name(s):
PP2A subunit B isoform B55-delta
PP2A subunit B isoform PR55-delta
PP2A subunit B isoform R2-delta
PP2A subunit B isoform delta
Gene names
Name:PPP2R2D
Synonyms:KIAA1541
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment By similarity.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with ENSA (when phosphorylated at 'Ser-67') and ARPP19 (when phosphorylated at 'Ser-62'), leading to inhibit PP2A activity By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B family.

Contains 7 WD repeats.

Sequence caution

The sequence BAA96065.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI16703.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform
PRO_0000071433

Regions

Repeat32 – 7140WD 1
Repeat97 – 13842WD 2
Repeat181 – 21939WD 3
Repeat230 – 27041WD 4
Repeat289 – 32739WD 5
Repeat344 – 38542WD 6
Repeat420 – 45233WD 7
Compositional bias3 – 86Poly-Gly

Natural variations

Natural variant3581G → S.
Corresponds to variant rs34473884 [ dbSNP | Ensembl ].
VAR_057127

Experimental info

Sequence conflict2901I → V in BAF85754. Ref.1
Sequence conflict3111Y → H in BAF85754. Ref.1
Sequence conflict3211M → T in BAF85754. Ref.1
Sequence conflict4491Q → R in BAF85754. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q66LE6 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 0669CDB80AF4400E

FASTA45352,042
        10         20         30         40         50         60 
MAGAGGGGCP AGGNDFQWCF SQVKGAIDED VAEADIISTV EFNYSGDLLA TGDKGGRVVI 

        70         80         90        100        110        120 
FQREQENKSR PHSRGEYNVY STFQSHEPEF DYLKSLEIEE KINKIRWLPQ QNAAHFLLST 

       130        140        150        160        170        180 
NDKTIKLWKI SERDKRAEGY NLKDEDGRLR DPFRITALRV PILKPMDLMV EASPRRIFAN 

       190        200        210        220        230        240 
AHTYHINSIS VNSDHETYLS ADDLRINLWH LEITDRSFNI VDIKPANMEE LTEVITAAEF 

       250        260        270        280        290        300 
HPHQCNVFVY SSSKGTIRLC DMRSSALCDR HSKFFEEPED PSSRSFFSEI ISSISDVKFS 

       310        320        330        340        350        360 
HSGRYMMTRD YLSVKVWDLN MESRPVETHQ VHEYLRSKLC SLYENDCIFD KFECCWNGSD 

       370        380        390        400        410        420 
SAIMTGSYNN FFRMFDRDTR RDVTLEASRE SSKPRASLKP RKVCTGGKRR KDEISVDSLD 

       430        440        450 
FNKKILHTAW HPVDNVIAVA ATNNLYIFQD KIN 

« Hide

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-453.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK293065 mRNA. Translation: BAF85754.1.
AL732395 Genomic DNA. Translation: CAI16703.1. Sequence problems.
BC047379 mRNA. Translation: AAH47379.1.
AB040974 mRNA. Translation: BAA96065.1. Different initiation.
RefSeqNP_060931.2. NM_018461.3.
UniGeneHs.380372.
Hs.657480.

3D structure databases

ProteinModelPortalQ66LE6.
SMRQ66LE6. Positions 14-452.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120946. 31 interactions.
IntActQ66LE6. 27 interactions.
MINTMINT-2820413.
STRING9606.ENSP00000381100.

PTM databases

PhosphoSiteQ66LE6.

Polymorphism databases

DMDM74736328.

Proteomic databases

PaxDbQ66LE6.
PRIDEQ66LE6.

Protocols and materials databases

DNASU55844.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000455566; ENSP00000399970; ENSG00000175470.
ENST00000595776; ENSP00000469896; ENSG00000269277.
GeneID55844.
KEGGhsa:55844.
UCSCuc001lks.3. human.

Organism-specific databases

CTD55844.
GeneCardsGC10P133747.
H-InvDBHIX0009317.
HGNCHGNC:23732. PPP2R2D.
MIM613992. gene.
neXtProtNX_Q66LE6.
PharmGKBPA134899040.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5170.
HOGENOMHOG000089745.
HOVERGENHBG000012.
InParanoidQ66LE6.
KOK04354.
PhylomeDBQ66LE6.
TreeFamTF105553.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

ArrayExpressQ66LE6.
BgeeQ66LE6.
CleanExHS_PPP2R2D.
GenevestigatorQ66LE6.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERPTHR11871. PTHR11871. 1 hit.
PIRSFPIRSF037309. PP2A_PR55. 1 hit.
PRINTSPR00600. PP2APR55.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 3 hits.
PROSITEPS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPP2R2D. human.
GenomeRNAi55844.
NextBio61085.
PROQ66LE6.
SOURCESearch...

Entry information

Entry name2ABD_HUMAN
AccessionPrimary (citable) accession number: Q66LE6
Secondary accession number(s): A8KAK0, Q5SQJ2, Q9P1Y7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM