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Protein

Calcium-binding mitochondrial carrier protein SCaMC-1

Gene

slc25a24

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi33 – 44121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi69 – 80122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi100 – 111123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi136 – 147124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein SCaMC-1
Alternative name(s):
Small calcium-binding mitochondrial carrier protein 1
Solute carrier family 25 member 24
Gene namesi
Name:slc25a24
Synonyms:scamc1
ORF Names:si:ch211-63o20.9, zgc:92470
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-040912-183. slc25a24.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 198198Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei199 – 21618Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini217 – 25337Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei254 – 27320Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini274 – 29623Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei297 – 31014Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini311 – 34636Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei347 – 36620Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini367 – 38923Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei390 – 40718Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini408 – 44639Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei447 – 46620Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini467 – 47711Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Calcium-binding mitochondrial carrier protein SCaMC-1PRO_0000317597Add
BLAST

Proteomic databases

PaxDbiQ66L49.

Expressioni

Gene expression databases

BgeeiQ66L49.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000033055.

Structurei

3D structure databases

ProteinModelPortaliQ66L49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 5536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini123 – 15836EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Repeati193 – 27987Solcar 1Add
BLAST
Repeati287 – 37286Solcar 2Add
BLAST
Repeati384 – 47289Solcar 3Add
BLAST

Domaini

The N-terminal domain can bind calcium and regulates the ATP carrier activity of the transmembrane domain. The apo form of the N-terminal domain is intrinsically disordered and binds to the transmembrane domain, leading to inhibition of the ATP carrier activity. Calcium binding leads to a major conformation change and abolishes the interaction with the transmembrane domain and the inhibition of the ATP carrier activity (By similarity).By similarity

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0036. Eukaryota.
ENOG410XQ4P. LUCA.
GeneTreeiENSGT00760000119245.
HOGENOMiHOG000165728.
HOVERGENiHBG108464.
InParanoidiQ66L49.
KOiK14684.
OMAiCIYPVET.
OrthoDBiEOG7V7661.
PhylomeDBiQ66L49.
TreeFamiTF313492.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.50.40.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002167. Graves_DC.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00928. GRAVESDC.
PR00926. MITOCARRIER.
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
SSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66L49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQLIRKFVF TESHCLEEED NTKSFAELFE KLDVNKDGKV DVSELKTGLA
60 70 80 90 100
AMGFSMGKGE AQKIVTSGDT DKDEGLDFEE FSKYLKEHEK KLRLTFKSLD
110 120 130 140 150
KNEDGRVDAK EIQQSLKDLG INLSDKDAEK ILHSIDVDGT MTLDWNEWRE
160 170 180 190 200
HFLFNPAEDL QQIIRYWKKS TVLDIGDSLT IPDEFTEEEK TTGMWWKQLA
210 220 230 240 250
AGGVAGAVSR TGTAPLDRMK VFMQVHSSKT NKISLVNGFK QMIKEGGVAS
260 270 280 290 300
LWRGNGVNVI KIAPETAIKF MAYEQYKKLL SKDGGKVQSH ERFMAGSLAG
310 320 330 340 350
ATAQTAIYPM EVMKTRLTLR KTGQYSGMFD CAKKILRKEG VKAFYKGYVP
360 370 380 390 400
NILGIIPYAG IDLAVYETLK NTWLSHYAKD TANPGVLVLL GCGTISSTCG
410 420 430 440 450
QLASYPLALI RTRMQAMASM EGSEQVSMSK LVKKIMQKEG FFGLYRGILP
460 470
NFMKVIPAVS ISYVVYEYMR SGLGISK
Length:477
Mass (Da):53,356
Last modified:October 11, 2004 - v1
Checksum:iC19A5B099747B5FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX279523 Genomic DNA. Translation: CAI12040.1.
BC078435 mRNA. Translation: AAH78435.1.
RefSeqiNP_001004606.1. NM_001004606.1.
UniGeneiDr.82440.

Genome annotation databases

EnsembliENSDART00000033325; ENSDARP00000033055; ENSDARG00000008568.
GeneIDi447867.
KEGGidre:447867.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX279523 Genomic DNA. Translation: CAI12040.1.
BC078435 mRNA. Translation: AAH78435.1.
RefSeqiNP_001004606.1. NM_001004606.1.
UniGeneiDr.82440.

3D structure databases

ProteinModelPortaliQ66L49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000033055.

Proteomic databases

PaxDbiQ66L49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000033325; ENSDARP00000033055; ENSDARG00000008568.
GeneIDi447867.
KEGGidre:447867.

Organism-specific databases

CTDi29957.
ZFINiZDB-GENE-040912-183. slc25a24.

Phylogenomic databases

eggNOGiKOG0036. Eukaryota.
ENOG410XQ4P. LUCA.
GeneTreeiENSGT00760000119245.
HOGENOMiHOG000165728.
HOVERGENiHBG108464.
InParanoidiQ66L49.
KOiK14684.
OMAiCIYPVET.
OrthoDBiEOG7V7661.
PhylomeDBiQ66L49.
TreeFamiTF313492.

Miscellaneous databases

PROiQ66L49.

Gene expression databases

BgeeiQ66L49.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.50.40.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002167. Graves_DC.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00928. GRAVESDC.
PR00926. MITOCARRIER.
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
SSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiSCMC1_DANRE
AccessioniPrimary (citable) accession number: Q66L49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.