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Protein

Mitogen-activated protein kinase kinase kinase 10

Gene

Map3k10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Activates the JUN N-terminal pathway.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251ATPPROSITE-ProRule annotation
Active sitei222 – 2221Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1129ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 10 (EC:2.7.11.25)
Gene namesi
Name:Map3k10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1346879. Map3k10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Mitogen-activated protein kinase kinase kinase 10PRO_0000277826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei258 – 2581Phosphothreonine; by autocatalysisBy similarity
Modified residuei262 – 2621Phosphoserine; by autocatalysis and MAP4K1By similarity
Modified residuei498 – 4981PhosphoserineBy similarity
Modified residuei502 – 5021PhosphoserineBy similarity
Modified residuei506 – 5061PhosphoserineBy similarity
Modified residuei552 – 5521PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ66L42.
PaxDbiQ66L42.
PRIDEiQ66L42.

PTM databases

PhosphoSiteiQ66L42.

Expressioni

Gene expression databases

BgeeiQ66L42.
CleanExiMM_MAP3K10.
ExpressionAtlasiQ66L42. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi234726. 1 interaction.
IntActiQ66L42. 1 interaction.
MINTiMINT-8414998.
STRINGi10090.ENSMUSP00000103978.

Structurei

3D structure databases

ProteinModelPortaliQ66L42.
SMRiQ66L42. Positions 17-78, 92-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 8166SH3PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 360263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 40522Leucine-zipper 1Add
BLAST
Regioni419 – 44022Leucine-zipper 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi763 – 915153Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000060081.
HOVERGENiHBG067662.
InParanoidiQ66L42.
KOiK04418.
OrthoDBiEOG7D85VN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR015785. MAP3K10.
IPR016231. MLK1/MLK2/MLK4.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23257:SF370. PTHR23257:SF370. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66L42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC
60 70 80 90 100
AVSGDEGWWT GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL
110 120 130 140 150
EEIIGVGGFG KVYRAVWRGE EVAVKAARLD PERDPAVTAE QVRQEARLFG
160 170 180 190 200
ALQHPNIIAL RGACLSPPNL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA
210 220 230 240 250
VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG
260 270 280 290 300
LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE
310 320 330 340 350
VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF
360 370 380 390 400
GSILKQLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS
410 420 430 440 450
REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMSQLSQEKP
460 470 480 490 500
RVRKRKGNFK RSRLLKLREG SSHISLPSGF EHKITVQASP TLDKRKGSDG
510 520 530 540 550
ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK EELVGGKKKG
560 570 580 590 600
RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF
610 620 630 640 650
ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV
660 670 680 690 700
TPSRPGHGAR RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS
710 720 730 740 750
LFFPRPGRFP RGLSPTGRPG GRREDTAPGL GLAPSATLVS LSSVSDCNST
760 770 780 790 800
RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN PLVDVELESF KKDPRQSLTP
810 820 830 840 850
THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD FPRLPDPQAL
860 870 880 890 900
FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS
910 920 930 940
RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH
Length:940
Mass (Da):103,187
Last modified:February 20, 2007 - v2
Checksum:iBE27F39679DC63D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti350 – 3534FGSI → PTRP in AAH46514 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074312 Genomic DNA. No translation available.
BC046514 mRNA. Translation: AAH46514.1.
BC078445 mRNA. Translation: AAH78445.1.
CCDSiCCDS39850.1.
RefSeqiNP_001277457.1. NM_001290528.1.
UniGeneiMm.389883.

Genome annotation databases

EnsembliENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390.
GeneIDi269881.
KEGGimmu:269881.
UCSCiuc009fwv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074312 Genomic DNA. No translation available.
BC046514 mRNA. Translation: AAH46514.1.
BC078445 mRNA. Translation: AAH78445.1.
CCDSiCCDS39850.1.
RefSeqiNP_001277457.1. NM_001290528.1.
UniGeneiMm.389883.

3D structure databases

ProteinModelPortaliQ66L42.
SMRiQ66L42. Positions 17-78, 92-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234726. 1 interaction.
IntActiQ66L42. 1 interaction.
MINTiMINT-8414998.
STRINGi10090.ENSMUSP00000103978.

PTM databases

PhosphoSiteiQ66L42.

Proteomic databases

MaxQBiQ66L42.
PaxDbiQ66L42.
PRIDEiQ66L42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390.
GeneIDi269881.
KEGGimmu:269881.
UCSCiuc009fwv.2. mouse.

Organism-specific databases

CTDi4294.
MGIiMGI:1346879. Map3k10.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000060081.
HOVERGENiHBG067662.
InParanoidiQ66L42.
KOiK04418.
OrthoDBiEOG7D85VN.

Miscellaneous databases

PROiQ66L42.
SOURCEiSearch...

Gene expression databases

BgeeiQ66L42.
CleanExiMM_MAP3K10.
ExpressionAtlasiQ66L42. baseline and differential.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR015785. MAP3K10.
IPR016231. MLK1/MLK2/MLK4.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23257:SF370. PTHR23257:SF370. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-940.
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.

Entry informationi

Entry nameiM3K10_MOUSE
AccessioniPrimary (citable) accession number: Q66L42
Secondary accession number(s): Q80UW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: July 22, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.