Reviewed, UniProtKB/Swiss-Prot Q66L42 (M3K10_MOUSE)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase 10
    EC=2.7.11.25

Gene names

Name:

Map3k10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	940 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates the JUN N-terminal pathway By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity.
Subunit structure	Homodimer By similarity.
Post-translational modification	Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	negative regulation of transcription Inferred from direct assay. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 940	940	Mitogen-activated protein kinase kinase kinase 10		PRO_0000277826
Regions
	Domain	16 – 81	66	SH3
	Domain	98 – 360	263	Protein kinase
	Domain	384 – 405	22	Leucine-zipper 1
	Domain	419 – 440	22	Leucine-zipper 2
	Nucleotide binding	104 – 112	9	ATP By similarity
	Compositional bias	763 – 915	153	Pro-rich
Sites
	Active site	222	1	Proton acceptor By similarity
	Binding site	125	1	ATP By similarity
Amino acid modifications
	Modified residue	258	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	262	1	Phosphoserine; by autocatalysis and MAP4K1 By similarity
	Modified residue	489	1	Phosphoserine By similarity
	Modified residue	498	1	Phosphoserine By similarity
	Modified residue	502	1	Phosphoserine By similarity
	Modified residue	506	1	Phosphoserine By similarity
	Modified residue	508	1	Phosphoserine By similarity
	Modified residue	552	1	Phosphothreonine By similarity
	Modified residue	582	1	Phosphoserine By similarity
	Modified residue	583	1	Phosphoserine By similarity
	Modified residue	816	1	Phosphothreonine By similarity
	Modified residue	818	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	350 – 353	4	FGSI → PTRP in AAH46514. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q66L42-1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: BE27F39679DC63D0	FASTA	940	103,187
	10 20 30 40 50 60 MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC AVSGDEGWWT 70 80 90 100 110 120 GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL EEIIGVGGFG KVYRAVWRGE 130 140 150 160 170 180 EVAVKAARLD PERDPAVTAE QVRQEARLFG ALQHPNIIAL RGACLSPPNL CLVMEYARGG 190 200 210 220 230 240 ALSRVLAGRR VPPHVLVNWA VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA 250 260 270 280 290 300 DTVLKITDFG LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE 310 320 330 340 350 360 VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF GSILKQLEVI 370 380 390 400 410 420 EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS REEELLRAAQ EQRFQEEQLR 430 440 450 460 470 480 RREQELAERE MDIVERELHL LMSQLSQEKP RVRKRKGNFK RSRLLKLREG SSHISLPSGF 490 500 510 520 530 540 EHKITVQASP TLDKRKGSDG ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK 550 560 570 580 590 600 EELVGGKKKG RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF 610 620 630 640 650 660 ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV TPSRPGHGAR 670 680 690 700 710 720 RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS LFFPRPGRFP RGLSPTGRPG 730 740 750 760 770 780 GRREDTAPGL GLAPSATLVS LSSVSDCNST RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN 790 800 810 820 830 840 PLVDVELESF KKDPRQSLTP THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD 850 860 870 880 890 900 FPRLPDPQAL FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS 910 920 930 940 RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-940. Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC074312 Genomic DNA. No translation available. BC046514 mRNA. Translation: AAH46514.1. BC078445 mRNA. Translation: AAH78445.1.
IPI	IPI00672525.
UniGene	Mm.389883
3D structure databases
HSSP	HSSP built from PDB template 2EVA based on UniProtKB O43318.
SMR	Q66L42. Positions 17-78, 19-360.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66L42.
PTM databases
PhosphoSite	Q66L42.
Proteomic databases
PRIDE	Q66L42.
Genome annotation databases
Ensembl	ENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1346879. Map3k10.
Phylogenomic databases
eggNOG	roNOG10743.
HOVERGEN	Q66L42.
OrthoDB	EOG9QC3RC.
PhylomeDB	Q66L42.
Enzyme and pathway databases
BRENDA	2.7.11.25. 244.
Gene expression databases
ArrayExpress	Q66L42.
Bgee	Q66L42.
CleanEx	MM_MAP3K10.
Genevestigator	Q66L42.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR015785. MAPKKK-like. IPR016231. MAPKKK9/10/11. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_prot_kinase_AS. IPR011511. SH3_2. IPR001452. SH3_domain. IPR020473. SH3_region. [Graphical view]
PANTHER	PTHR23257:SF87. MAPKKK-like. 1 hit.
Pfam	PF07653. SH3_2. 1 hit. [Graphical view]
PIRSF	PIRSF000556. MAPKKK9_11. 1 hit.
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

M3K10_MOUSE

Accession

Primary (citable) accession number: Q66L42
Secondary accession number(s): Q80UW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 20, 2007
Last modified:	February 9, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)