Mitogen-activated protein kinase kinase kinase 10

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Q66L42 (M3K10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 10
EC=2.7.11.25

Gene names

Name:

Map3k10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	940 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates the JUN N-terminal pathway By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity.
Subunit structure	Homodimer By similarity.
Post-translational modification	Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC transcription corepressor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 940

940

Mitogen-activated protein kinase kinase kinase 10

PRO_0000277826

Regions

Domain

16 – 81

SH3

Domain

98 – 360

263

Protein kinase

Domain

384 – 405

Leucine-zipper 1

Domain

419 – 440

Leucine-zipper 2

Nucleotide binding

104 – 112

ATP By similarity

Compositional bias

763 – 915

153

Pro-rich

Sites

Active site

222

Proton acceptor By similarity

Binding site

125

ATP By similarity

Amino acid modifications

Modified residue

258

Phosphothreonine; by autocatalysis By similarity

Modified residue

262

Phosphoserine; by autocatalysis and MAP4K1 By similarity

Modified residue

489

Phosphoserine By similarity

Modified residue

498

Phosphoserine By similarity

Modified residue

502

Phosphoserine By similarity

Modified residue

506

Phosphoserine By similarity

Modified residue

508

Phosphoserine By similarity

Modified residue

552

Phosphothreonine By similarity

Modified residue

582

Phosphoserine By similarity

Modified residue

583

Phosphoserine By similarity

Modified residue

816

Phosphothreonine By similarity

Modified residue

818

Phosphoserine By similarity

Experimental info

Sequence conflict

350 – 353

FGSI → PTRP in AAH46514. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q66L42 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: BE27F39679DC63D0
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FASTA

940

103,187

        10         20         30         40         50         60 
MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC AVSGDEGWWT 

        70         80         90        100        110        120 
GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL EEIIGVGGFG KVYRAVWRGE 

       130        140        150        160        170        180 
EVAVKAARLD PERDPAVTAE QVRQEARLFG ALQHPNIIAL RGACLSPPNL CLVMEYARGG 

       190        200        210        220        230        240 
ALSRVLAGRR VPPHVLVNWA VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA 

       250        260        270        280        290        300 
DTVLKITDFG LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE 

       310        320        330        340        350        360 
VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF GSILKQLEVI 

       370        380        390        400        410        420 
EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS REEELLRAAQ EQRFQEEQLR 

       430        440        450        460        470        480 
RREQELAERE MDIVERELHL LMSQLSQEKP RVRKRKGNFK RSRLLKLREG SSHISLPSGF 

       490        500        510        520        530        540 
EHKITVQASP TLDKRKGSDG ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK 

       550        560        570        580        590        600 
EELVGGKKKG RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF 

       610        620        630        640        650        660 
ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV TPSRPGHGAR 

       670        680        690        700        710        720 
RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS LFFPRPGRFP RGLSPTGRPG 

       730        740        750        760        770        780 
GRREDTAPGL GLAPSATLVS LSSVSDCNST RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN 

       790        800        810        820        830        840 
PLVDVELESF KKDPRQSLTP THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD 

       850        860        870        880        890        900 
FPRLPDPQAL FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS 

       910        920        930        940 
RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH

« Hide

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-940. Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC074312 Genomic DNA. No translation available. BC046514 mRNA. Translation: AAH46514.1. BC078445 mRNA. Translation: AAH78445.1.
IPI	IPI00672525.
UniGene	Mm.389883.
3D structure databases
HSSP	HSSP built from PDB template 2EVA based on UniProtKB O43318.
ProteinModelPortal	Q66L42.
SMR	Q66L42. Positions 13-361, 415-473.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66L42.
PTM databases
PhosphoSite	Q66L42.
Proteomic databases
PRIDE	Q66L42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390.
UCSC	uc012fgl.1. mouse.
Organism-specific databases
MGI	MGI:1346879. Map3k10.
Phylogenomic databases
eggNOG	roNOG10743.
GeneTree	ENSGT00600000084346.
HOVERGEN	HBG067662.
OrthoDB	EOG4NCMC3.
PhylomeDB	Q66L42.
Gene expression databases
ArrayExpress	Q66L42.
Bgee	Q66L42.
CleanEx	MM_MAP3K10.
Genevestigator	Q66L42.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR016231. MAPKKK9/10/11. IPR015785. MAPKKK9/10/11-like. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. IPR001452. SH3_domain. [Graphical view]
PANTHER	PTHR23257:SF87. MAPKKK-like. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000556. MAPKKK9_11. 1 hit.
PRINTS	PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

M3K10_MOUSE

Accession

Primary (citable) accession number: Q66L42
Secondary accession number(s): Q80UW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 20, 2007
Last modified:	September 21, 2011
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents