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Q66L42 (M3K10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase kinase kinase 10

EC=2.7.11.25
Gene names
Name:Map3k10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length940 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activates the JUN N-terminal pathway By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity.

Subunit structure

Homodimer By similarity.

Post-translational modification

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 940940Mitogen-activated protein kinase kinase kinase 10
PRO_0000277826

Regions

Domain16 – 8166SH3
Domain98 – 360263Protein kinase
Nucleotide binding104 – 1129ATP By similarity
Region384 – 40522Leucine-zipper 1
Region419 – 44022Leucine-zipper 2
Compositional bias763 – 915153Pro-rich

Sites

Active site2221Proton acceptor By similarity
Binding site1251ATP By similarity

Amino acid modifications

Modified residue2581Phosphothreonine; by autocatalysis By similarity
Modified residue2621Phosphoserine; by autocatalysis and MAP4K1 By similarity
Modified residue4981Phosphoserine By similarity
Modified residue5021Phosphoserine By similarity
Modified residue5061Phosphoserine By similarity
Modified residue5521Phosphothreonine By similarity

Experimental info

Sequence conflict350 – 3534FGSI → PTRP in AAH46514. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q66L42 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: BE27F39679DC63D0

FASTA940103,187
        10         20         30         40         50         60 
MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC AVSGDEGWWT 

        70         80         90        100        110        120 
GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL EEIIGVGGFG KVYRAVWRGE 

       130        140        150        160        170        180 
EVAVKAARLD PERDPAVTAE QVRQEARLFG ALQHPNIIAL RGACLSPPNL CLVMEYARGG 

       190        200        210        220        230        240 
ALSRVLAGRR VPPHVLVNWA VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA 

       250        260        270        280        290        300 
DTVLKITDFG LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE 

       310        320        330        340        350        360 
VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF GSILKQLEVI 

       370        380        390        400        410        420 
EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS REEELLRAAQ EQRFQEEQLR 

       430        440        450        460        470        480 
RREQELAERE MDIVERELHL LMSQLSQEKP RVRKRKGNFK RSRLLKLREG SSHISLPSGF 

       490        500        510        520        530        540 
EHKITVQASP TLDKRKGSDG ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK 

       550        560        570        580        590        600 
EELVGGKKKG RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF 

       610        620        630        640        650        660 
ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV TPSRPGHGAR 

       670        680        690        700        710        720 
RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS LFFPRPGRFP RGLSPTGRPG 

       730        740        750        760        770        780 
GRREDTAPGL GLAPSATLVS LSSVSDCNST RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN 

       790        800        810        820        830        840 
PLVDVELESF KKDPRQSLTP THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD 

       850        860        870        880        890        900 
FPRLPDPQAL FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS 

       910        920        930        940 
RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-940.
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC074312 Genomic DNA. No translation available.
BC046514 mRNA. Translation: AAH46514.1.
BC078445 mRNA. Translation: AAH78445.1.
RefSeqNP_001277457.1. NM_001290528.1.
UniGeneMm.389883.

3D structure databases

ProteinModelPortalQ66L42.
SMRQ66L42. Positions 17-78, 87-428.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ66L42. 1 interaction.
MINTMINT-8414998.
STRING10090.ENSMUSP00000103978.

PTM databases

PhosphoSiteQ66L42.

Proteomic databases

PaxDbQ66L42.
PRIDEQ66L42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390.
GeneID269881.
KEGGmmu:269881.
UCSCuc012fgl.1. mouse.

Organism-specific databases

CTD4294.
MGIMGI:1346879. Map3k10.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117425.
HOGENOMHOG000060081.
HOVERGENHBG067662.
KOK04418.
OrthoDBEOG7D85VN.

Gene expression databases

BgeeQ66L42.
CleanExMM_MAP3K10.
GenevestigatorQ66L42.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR015785. MAP3K10.
IPR016231. MAPKKK9/10/11.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR23257:SF370. PTHR23257:SF370. 1 hit.
PfamPF07714. Pkinase_Tyr. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFPIRSF000556. MAPKKK9_11. 1 hit.
PRINTSPR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ66L42.
SOURCESearch...

Entry information

Entry nameM3K10_MOUSE
AccessionPrimary (citable) accession number: Q66L42
Secondary accession number(s): Q80UW4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot