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Q66L42

- M3K10_MOUSE

UniProt

Q66L42 - M3K10_MOUSE

Protein

Mitogen-activated protein kinase kinase kinase 10

Gene

Map3k10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Activates the JUN N-terminal pathway.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251ATPPROSITE-ProRule annotation
    Active sitei222 – 2221Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1129ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase kinase kinase activity Source: UniProtKB-EC
    3. protein kinase activity Source: MGI
    4. transcription corepressor activity Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase 10 (EC:2.7.11.25)
    Gene namesi
    Name:Map3k10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1346879. Map3k10.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Mitogen-activated protein kinase kinase kinase 10PRO_0000277826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei258 – 2581Phosphothreonine; by autocatalysisBy similarity
    Modified residuei262 – 2621Phosphoserine; by autocatalysis and MAP4K1By similarity
    Modified residuei498 – 4981PhosphoserineBy similarity
    Modified residuei502 – 5021PhosphoserineBy similarity
    Modified residuei506 – 5061PhosphoserineBy similarity
    Modified residuei552 – 5521PhosphothreonineBy similarity

    Post-translational modificationi

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ66L42.
    PRIDEiQ66L42.

    PTM databases

    PhosphoSiteiQ66L42.

    Expressioni

    Gene expression databases

    BgeeiQ66L42.
    CleanExiMM_MAP3K10.
    GenevestigatoriQ66L42.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ66L42. 1 interaction.
    MINTiMINT-8414998.
    STRINGi10090.ENSMUSP00000103978.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66L42.
    SMRiQ66L42. Positions 17-78, 87-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 8166SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini98 – 360263Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni384 – 40522Leucine-zipper 1Add
    BLAST
    Regioni419 – 44022Leucine-zipper 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi763 – 915153Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117425.
    HOGENOMiHOG000060081.
    HOVERGENiHBG067662.
    OrthoDBiEOG7D85VN.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR015785. MAP3K10.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR23257:SF370. PTHR23257:SF370. 1 hit.
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSiPR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q66L42-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC    50
    AVSGDEGWWT GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL 100
    EEIIGVGGFG KVYRAVWRGE EVAVKAARLD PERDPAVTAE QVRQEARLFG 150
    ALQHPNIIAL RGACLSPPNL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA 200
    VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG 250
    LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE 300
    VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF 350
    GSILKQLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS 400
    REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMSQLSQEKP 450
    RVRKRKGNFK RSRLLKLREG SSHISLPSGF EHKITVQASP TLDKRKGSDG 500
    ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK EELVGGKKKG 550
    RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF 600
    ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV 650
    TPSRPGHGAR RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS 700
    LFFPRPGRFP RGLSPTGRPG GRREDTAPGL GLAPSATLVS LSSVSDCNST 750
    RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN PLVDVELESF KKDPRQSLTP 800
    THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD FPRLPDPQAL 850
    FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS 900
    RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH 940
    Length:940
    Mass (Da):103,187
    Last modified:February 20, 2007 - v2
    Checksum:iBE27F39679DC63D0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti350 – 3534FGSI → PTRP in AAH46514. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC074312 Genomic DNA. No translation available.
    BC046514 mRNA. Translation: AAH46514.1.
    BC078445 mRNA. Translation: AAH78445.1.
    CCDSiCCDS39850.1.
    RefSeqiNP_001277457.1. NM_001290528.1.
    UniGeneiMm.389883.

    Genome annotation databases

    EnsembliENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390.
    GeneIDi269881.
    UCSCiuc012fgl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC074312 Genomic DNA. No translation available.
    BC046514 mRNA. Translation: AAH46514.1 .
    BC078445 mRNA. Translation: AAH78445.1 .
    CCDSi CCDS39850.1.
    RefSeqi NP_001277457.1. NM_001290528.1.
    UniGenei Mm.389883.

    3D structure databases

    ProteinModelPortali Q66L42.
    SMRi Q66L42. Positions 17-78, 87-428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q66L42. 1 interaction.
    MINTi MINT-8414998.
    STRINGi 10090.ENSMUSP00000103978.

    PTM databases

    PhosphoSitei Q66L42.

    Proteomic databases

    PaxDbi Q66L42.
    PRIDEi Q66L42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036453 ; ENSMUSP00000037725 ; ENSMUSG00000040390 .
    GeneIDi 269881.
    UCSCi uc012fgl.1. mouse.

    Organism-specific databases

    MGIi MGI:1346879. Map3k10.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117425.
    HOGENOMi HOG000060081.
    HOVERGENi HBG067662.
    OrthoDBi EOG7D85VN.

    Miscellaneous databases

    PROi Q66L42.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q66L42.
    CleanExi MM_MAP3K10.
    Genevestigatori Q66L42.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR015785. MAP3K10.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR23257:SF370. PTHR23257:SF370. 1 hit.
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSi PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 136-940.
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary tumor.

    Entry informationi

    Entry nameiM3K10_MOUSE
    AccessioniPrimary (citable) accession number: Q66L42
    Secondary accession number(s): Q80UW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3