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Protein

Mitogen-activated protein kinase kinase kinase 10

Gene

Map3k10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates the JUN N-terminal pathway.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125ATPPROSITE-ProRule annotation1
Active sitei222Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 112ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 10 (EC:2.7.11.25)
Gene namesi
Name:Map3k10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1346879 Map3k10

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002778261 – 940Mitogen-activated protein kinase kinase kinase 10Add BLAST940

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei258Phosphothreonine; by autocatalysisBy similarity1
Modified residuei262Phosphoserine; by autocatalysis and MAP4K1By similarity1
Modified residuei498PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1
Modified residuei506PhosphoserineCombined sources1
Modified residuei552PhosphothreonineCombined sources1
Modified residuei843Omega-N-methylarginineCombined sources1

Post-translational modificationi

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ66L42
PaxDbiQ66L42
PRIDEiQ66L42

PTM databases

iPTMnetiQ66L42
PhosphoSitePlusiQ66L42

Expressioni

Gene expression databases

BgeeiENSMUSG00000040390
CleanExiMM_MAP3K10
ExpressionAtlasiQ66L42 baseline and differential
GenevisibleiQ66L42 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234726, 1 interactor
IntActiQ66L42, 1 interactor
MINTiQ66L42
STRINGi10090.ENSMUSP00000103978

Structurei

3D structure databases

ProteinModelPortaliQ66L42
SMRiQ66L42
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 81SH3PROSITE-ProRule annotationAdd BLAST66
Domaini98 – 360Protein kinasePROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni384 – 405Leucine-zipper 1Add BLAST22
Regioni419 – 440Leucine-zipper 2Add BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi763 – 915Pro-richAdd BLAST153

Sequence similaritiesi

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00900000140790
HOGENOMiHOG000060081
HOVERGENiHBG067662
InParanoidiQ66L42
KOiK04418

Family and domain databases

CDDicd12059 SH3_MLK1-3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR015785 MAP3K10
IPR035779 MLK1-3_SH3
IPR016231 MLK1-4
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43997:SF5 PTHR43997:SF5, 1 hit
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF14604 SH3_9, 1 hit
PIRSFiPIRSF000556 MAPKKK9_11, 1 hit
PRINTSiPR00452 SH3DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q66L42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEEGAAAR EWGATPAGPV WTAVFDYEAV GDEELTLRRG DRVQVLSQDC
60 70 80 90 100
AVSGDEGWWT GQLPSGRVGV FPSNYVAPAA PAAPSDLQLP QEIPFHELQL
110 120 130 140 150
EEIIGVGGFG KVYRAVWRGE EVAVKAARLD PERDPAVTAE QVRQEARLFG
160 170 180 190 200
ALQHPNIIAL RGACLSPPNL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA
210 220 230 240 250
VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG
260 270 280 290 300
LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE
310 320 330 340 350
VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF
360 370 380 390 400
GSILKQLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS
410 420 430 440 450
REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMSQLSQEKP
460 470 480 490 500
RVRKRKGNFK RSRLLKLREG SSHISLPSGF EHKITVQASP TLDKRKGSDG
510 520 530 540 550
ASPPASPSII PRLRAIRLTP MDCGGSSGSG TWSRSGPPKK EELVGGKKKG
560 570 580 590 600
RTWGPSSTLQ KERAGGEERL KALGEGSKQW SSSAPNLGKS PKHTPMAPGF
610 620 630 640 650
ASLNEMEEFA EADEGNNVPP SPYSTPSYLK VPLPAEPSPC VQAPWEPPAV
660 670 680 690 700
TPSRPGHGAR RRCDLALLSC ATLLSAVGLG ADVAEARAGD GEEQRRWLDS
710 720 730 740 750
LFFPRPGRFP RGLSPTGRPG GRREDTAPGL GLAPSATLVS LSSVSDCNST
760 770 780 790 800
RSLLRSDSDE AAPAAPSPPP SPLAPSPSTN PLVDVELESF KKDPRQSLTP
810 820 830 840 850
THVTAAHAVS RGHRRTPSDG ALRQREPLEL TNHGPRDPLD FPRLPDPQAL
860 870 880 890 900
FPTRRRPLEF PGRPTTLTFA PRPRPAASRP RLDPWKLVSF GRTLSISPPS
910 920 930 940
RPDTPESPGP PSVQPTLLDM DMEGQSQDNT VPLCGVYGSH
Length:940
Mass (Da):103,187
Last modified:February 20, 2007 - v2
Checksum:iBE27F39679DC63D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350 – 353FGSI → PTRP in AAH46514 (PubMed:15489334).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074312 Genomic DNA No translation available.
BC046514 mRNA Translation: AAH46514.1
BC078445 mRNA Translation: AAH78445.1
CCDSiCCDS39850.2
RefSeqiNP_001277457.1, NM_001290528.1
UniGeneiMm.389883

Genome annotation databases

EnsembliENSMUST00000036453; ENSMUSP00000037725; ENSMUSG00000040390
GeneIDi269881
KEGGimmu:269881
UCSCiuc009fwv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiM3K10_MOUSE
AccessioniPrimary (citable) accession number: Q66L42
Secondary accession number(s): Q80UW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: June 20, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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