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Q66KH9

- CDK8_XENLA

UniProt

Q66KH9 - CDK8_XENLA

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Protein

Cyclin-dependent kinase 8

Gene
cdk8
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATP By similarity
Active sitei151 – 1511Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 8 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk8
Mediator of RNA polymerase II transcription subunit cdk8
Gene namesi
Name:cdk8
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-962272. cdk8.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Cyclin-dependent kinase 8PRO_0000312927Add
BLAST

Interactioni

Subunit structurei

Component of the Mediator complex By similarity. Interacts with ccnc By similarity.

Protein-protein interaction databases

IntActiQ66KH9. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ66KH9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 287267Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1515Interaction with CCNC By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi325 – 3295Poly-Gln

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652.
KOiK02208.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66KH9-1 [UniParc]FASTAAdd to Basket

« Hide

MDYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDRDYA    50
LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDFA 100
EHDLWHIIKF HRASKANKKP VQLPRGMVKS LLYQILDGIH YLHANWVLHR 150
DLKPANILVM GEGPERGRVK IADMGFARLF NSPLKPLADL DPVVVTFWYR 200
APELLLGARH YTKAIDKDWE DIKKMPEHST LIKDFRRNTY TNCSLIKYME 250
KHKVKPDSKT FHLLQKLLTM DPIKRISSEQ AMQDPYFLED PLPTSDVFAG 300
CQIPYPKREF LTEEEPDDKG DKKNQQQQQG NNHTNGTGHP GNQDNSHAQG 350
PPLKKVRVVP PTTTSGGLIM TSDYQRSNPH AAYQNPGPST SQPQSSMGYT 400
STSQQPPQYS HQTHRY 416
Length:416
Mass (Da):47,763
Last modified:October 11, 2004 - v1
Checksum:iF5524A35EF0A0BF3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC080386 mRNA. Translation: AAH80386.1.
RefSeqiNP_001087585.1. NM_001094116.1.
UniGeneiXl.48655.

Genome annotation databases

GeneIDi447409.
KEGGixla:447409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC080386 mRNA. Translation: AAH80386.1 .
RefSeqi NP_001087585.1. NM_001094116.1.
UniGenei Xl.48655.

3D structure databases

ProteinModelPortali Q66KH9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q66KH9. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 447409.
KEGGi xla:447409.

Organism-specific databases

CTDi 1024.
Xenbasei XB-GENE-962272. cdk8.

Phylogenomic databases

HOVERGENi HBG014652.
KOi K02208.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiCDK8_XENLA
AccessioniPrimary (citable) accession number: Q66KH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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