Q66KH9 (CDK8_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 8
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 8
Mediator complex subunit cdk8
Mediator of RNA polymerase II transcription subunit cdk8

Gene names

Name:

cdk8

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the Mediator complex By similarity. Interacts with ccnc By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Cyclin-dependent kinase 8		PRO_0000312927
Regions
	Domain	21 – 287	267	Protein kinase
	Nucleotide binding	27 – 35	9	ATP By similarity
	Region	1 – 15	15	Interaction with CCNC By similarity
	Compositional bias	325 – 329	5	Poly-Gln
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66KH9 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F5524A35EF0A0BF3	FASTA	416	47,763
	10 20 30 40 50 60 MDYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDRDYA LKQIEGTGIS 70 80 90 100 110 120 MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDFA EHDLWHIIKF HRASKANKKP 130 140 150 160 170 180 VQLPRGMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLF 190 200 210 220 230 240 NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDKDWE DIKKMPEHST LIKDFRRNTY 250 260 270 280 290 300 TNCSLIKYME KHKVKPDSKT FHLLQKLLTM DPIKRISSEQ AMQDPYFLED PLPTSDVFAG 310 320 330 340 350 360 CQIPYPKREF LTEEEPDDKG DKKNQQQQQG NNHTNGTGHP GNQDNSHAQG PPLKKVRVVP 370 380 390 400 410 PTTTSGGLIM TSDYQRSNPH AAYQNPGPST SQPQSSMGYT STSQQPPQYS HQTHRY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC080386 mRNA. Translation: AAH80386.1.
RefSeq	NP_001087585.1. NM_001094116.1.
UniGene	Xl.48655.
3D structure databases
HSSP	HSSP built from PDB template 1OIT based on UniProtKB P24941.
ProteinModelPortal	Q66KH9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q66KH9. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	447409.
KEGG	xla:447409.
Organism-specific databases
CTD	1024.
Xenbase	XB-GENE-962272. cdk8.
Phylogenomic databases
HOVERGEN	HBG014652.
KO	K02208.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK8_XENLA

Accession

Primary (citable) accession number: Q66KH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program