Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carboxypeptidase Z

Gene

CPZ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves substrates with C-terminal arginine residues. Probably modulates the Wnt signaling pathway, by cleaving some undefined protein. May play a role in cleavage during prohormone processing.3 Publications

Cofactori

Zn2+By similarity

Enzyme regulationi

Inhibited by 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid (MGTA) and guanidinoethylmercaptosuccinic acid (GEMSA). Inhibited by chelating agents such as EDTA and EGTA.1 Publication

Kineticsi

  1. KM=2 mM for dansyl-Phe-Ala-Arg2 Publications
  2. KM=2 mM for dansyl-Pro-Ala-Arg2 Publications

    pH dependencei

    Optimum pH is 7.8.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi248Zinc; catalyticBy similarity1
    Metal bindingi251Zinc; catalyticBy similarity1
    Metal bindingi380Zinc; catalyticBy similarity1
    Active sitei472Proton donor/acceptorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • peptide metabolic process Source: GO_Central
    • protein processing Source: GO_Central
    • proteolysis Source: ProtInc
    • Wnt signaling pathway Source: UniProtKB-KW

    Keywordsi

    Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
    Biological processWnt signaling pathway
    LigandMetal-binding, Zinc

    Protein family/group databases

    MEROPSiM14.012

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxypeptidase Z (EC:3.4.17.-)
    Short name:
    CPZ
    Gene namesi
    Name:CPZ
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 4

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000109625.18
    HGNCiHGNC:2333 CPZ
    MIMi603105 gene
    neXtProtiNX_Q66K79

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi8532
    OpenTargetsiENSG00000109625
    PharmGKBiPA26854

    Polymorphism and mutation databases

    BioMutaiCPZ
    DMDMi296434423

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 18Sequence analysisAdd BLAST18
    ChainiPRO_000025245619 – 652Carboxypeptidase ZBy similarityAdd BLAST634

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi43 ↔ 109PROSITE-ProRule annotation
    Disulfide bondi51 ↔ 102PROSITE-ProRule annotation
    Disulfide bondi93 ↔ 129PROSITE-ProRule annotation
    Disulfide bondi118 ↔ 157PROSITE-ProRule annotation
    Disulfide bondi122 ↔ 146PROSITE-ProRule annotation
    Glycosylationi281N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ66K79
    PeptideAtlasiQ66K79
    PRIDEiQ66K79

    PTM databases

    iPTMnetiQ66K79
    PhosphoSitePlusiQ66K79

    Expressioni

    Tissue specificityi

    In placenta, it is present within invasive trophoblasts and in the surrounding extracellular space. Also present in amnion cells, but is not readily apparent in the extracellular matrix of this cell type. Present in normal pituitary gland and neoplastic pituitary gland (especially POMC-, GH- and PRL-producing adenomas) (at protein level). Widely expressed.3 Publications

    Gene expression databases

    BgeeiENSG00000109625
    CleanExiHS_CPZ
    ExpressionAtlasiQ66K79 baseline and differential
    GenevisibleiQ66K79 HS

    Interactioni

    Protein-protein interaction databases

    BioGridi114102, 3 interactors
    STRINGi9606.ENSP00000354255

    Structurei

    3D structure databases

    ProteinModelPortaliQ66K79
    SMRiQ66K79
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini27 – 160FZPROSITE-ProRule annotationAdd BLAST134

    Sequence similaritiesi

    Belongs to the peptidase M14 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG2649 Eukaryota
    ENOG410XX0H LUCA
    GeneTreeiENSGT00760000119124
    HOGENOMiHOG000232185
    HOVERGENiHBG003410
    InParanoidiQ66K79
    KOiK13022
    OMAiQHELMEP
    OrthoDBiEOG091G06A9
    PhylomeDBiQ66K79
    TreeFamiTF315592

    Family and domain databases

    CDDicd03867 M14_CPZ, 1 hit
    Gene3Di1.10.2000.10, 1 hit
    InterProiView protein in InterPro
    IPR008969 CarboxyPept-like_regulatory
    IPR020067 Frizzled_dom
    IPR036790 Frizzled_dom_sf
    IPR034239 M14_CPZ_CPD
    IPR000834 Peptidase_M14
    PfamiView protein in Pfam
    PF01392 Fz, 1 hit
    PF00246 Peptidase_M14, 1 hit
    PRINTSiPR00765 CRBOXYPTASEA
    SMARTiView protein in SMART
    SM00063 FRI, 1 hit
    SM00631 Zn_pept, 1 hit
    SUPFAMiSSF49464 SSF49464, 1 hit
    SSF63501 SSF63501, 1 hit
    PROSITEiView protein in PROSITE
    PS00133 CARBOXYPEPT_ZN_2, 1 hit
    PS50038 FZ, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q66K79-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MPPPLPLLLL TVLVVAAARP GCEFERNPAG ECHRPPAADS ATCVDLQLRT
    60 70 80 90 100
    CSDAAYNHTT FPNLLQHRSW EVVEASSEYI LLSVLHQLLE GQCNPDLRLL
    110 120 130 140 150
    GCAVLAPRCE GGWVRRPCRH ICEGLREVCQ PAFDAIDMAW PYFLDCHRYF
    160 170 180 190 200
    TREDEGCYDP LEKLRGGLEA DEALPSGLPP TFIRFSHHSY AQMVRVLRRT
    210 220 230 240 250
    ASRCAHVART YSIGRSFDGR ELLVIEFSSR PGQHELMEPE VKLIGNIHGN
    260 270 280 290 300
    EVAGREMLIY LAQYLCSEYL LGNPRIQRLL NTTRIHLLPS MNPDGYEVAA
    310 320 330 340 350
    AEGAGYNGWT SGRQNAQNLD LNRNFPDLTS EYYRLAETRG ARSDHIPIPQ
    360 370 380 390 400
    HYWWGKVAPE TKAIMKWMQT IPFVLSASLH GGDLVVSYPF DFSKHPQEEK
    410 420 430 440 450
    MFSPTPDEKM FKLLSRAYAD VHPMMMDRSE NRCGGNFLKR GSIINGADWY
    460 470 480 490 500
    SFTGGMSDFN YLHTNCFEIT VELGCVKFPP EEALYILWQH NKESLLNFVE
    510 520 530 540 550
    TVHRGIKGVV TDKFGKPVKN ARISVKGIRH DITTAPDGDY WRLLPPGIHI
    560 570 580 590 600
    VIAQAPGYAK VIKKVIIPAR MKRAGRVDFI LQPLGMGPKN FIHGLRRTGP
    610 620 630 640 650
    HDPLGGASSL GEATEPDPLR ARRQPSADGS KPWWWSYFTS LSTHRPRWLL

    KY
    Length:652
    Mass (Da):73,655
    Last modified:May 18, 2010 - v2
    Checksum:iBFA1EA7753E63F98
    GO
    Isoform 2 (identifier: Q66K79-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         30-40: Missing.

    Show »
    Length:641
    Mass (Da):72,534
    Checksum:iE88E3425C36F1576
    GO
    Isoform 3 (identifier: Q66K79-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-137: Missing.

    Show »
    Length:515
    Mass (Da):58,591
    Checksum:i50FB57968AB4B212
    GO

    Sequence cautioni

    The sequence BAB71147 differs from that shown. Reason: Frameshift at position 358.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti200T → M in BC006393 (PubMed:15489334).Curated1
    Sequence conflicti291M → I in AAB58911 (PubMed:9099699).Curated1
    Sequence conflicti523I → T in BAB71147 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_0278835L → P2 PublicationsCorresponds to variant dbSNP:rs2302583Ensembl.1
    Natural variantiVAR_0472446P → L1 PublicationCorresponds to variant dbSNP:rs34964084Ensembl.1
    Natural variantiVAR_047245130Q → L. Corresponds to variant dbSNP:rs35993494Ensembl.1
    Natural variantiVAR_047246486I → T2 PublicationsCorresponds to variant dbSNP:rs7378066Ensembl.1
    Natural variantiVAR_027884501T → M. Corresponds to variant dbSNP:rs9991535Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0403561 – 137Missing in isoform 3. 1 PublicationAdd BLAST137
    Alternative sequenceiVSP_02098330 – 40Missing in isoform 2. 1 PublicationAdd BLAST11

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U83411 mRNA Translation: AAB58911.1
    AK056317 mRNA Translation: BAB71147.1 Frameshift.
    AC105345 Genomic DNA No translation available.
    BC006393 mRNA No translation available.
    BC080539 mRNA Translation: AAH80539.1
    CCDSiCCDS33953.1 [Q66K79-1]
    CCDS3404.1 [Q66K79-2]
    CCDS43212.1 [Q66K79-3]
    RefSeqiNP_001014447.1, NM_001014447.2
    NP_001014448.1, NM_001014448.2
    NP_003643.2, NM_003652.3
    UniGeneiHs.61995

    Genome annotation databases

    EnsembliENST00000315782; ENSP00000315074; ENSG00000109625 [Q66K79-2]
    ENST00000360986; ENSP00000354255; ENSG00000109625 [Q66K79-1]
    ENST00000382480; ENSP00000371920; ENSG00000109625 [Q66K79-3]
    GeneIDi8532
    KEGGihsa:8532
    UCSCiuc003glm.4 human [Q66K79-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiCBPZ_HUMAN
    AccessioniPrimary (citable) accession number: Q66K79
    Secondary accession number(s): O00520, Q96MX2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: May 18, 2010
    Last modified: May 23, 2018
    This is version 116 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health