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Protein

Cartilage intermediate layer protein 1

Gene

Cilp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cartilage intermediate layer protein 1
Short name:
CILP-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cilp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2444507. Cilp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001467422 – 1184Cartilage intermediate layer protein 1Add BLAST1163
ChainiPRO_000001467522 – ?724Cartilage intermediate layer protein 1 C1Add BLAST703
ChainiPRO_0000014676?725 – 1184Cartilage intermediate layer protein 1 C2Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi132N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi165 ↔ 200By similarity
Disulfide bondi177 ↔ 185By similarity
Disulfide bondi330 ↔ 376By similarity
Glycosylationi346N-linked (GlcNAc...)By similarity1
Glycosylationi420N-linked (GlcNAc...)Sequence analysis1
Glycosylationi550N-linked (GlcNAc...)Sequence analysis1
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1000N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1056N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ66K08.
PaxDbiQ66K08.
PeptideAtlasiQ66K08.
PRIDEiQ66K08.

PTM databases

iPTMnetiQ66K08.
PhosphoSitePlusiQ66K08.

Expressioni

Tissue specificityi

Expressed in articular and meniscal cartilage (at protein level). Primarily localizes to the superficial and intermediate zones of articular cartilage (at protein level).1 Publication

Developmental stagei

Detected in articular cartilage from 8 weeks of age, but not at earlier stages (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000042254.

Interactioni

Subunit structurei

Monomer. Interacts with TGFB1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036631.

Structurei

3D structure databases

ProteinModelPortaliQ66K08.
SMRiQ66K08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 201TSP type-1PROSITE-ProRule annotationAdd BLAST52
Domaini309 – 393Ig-like C2-typeAdd BLAST85

Sequence similaritiesi

Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiENOG410IJBD. Eukaryota.
ENOG410XXZR. LUCA.
HOGENOMiHOG000111676.
HOVERGENiHBG081175.
InParanoidiQ66K08.
PhylomeDBiQ66K08.
TreeFamiTF330132.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000884. TSP1_rpt.
IPR025155. WxxW_domain.
[Graphical view]
PfamiPF13330. Mucin2_WxxW. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66K08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIKTWVFS FLVLEVTTVL GRQTMLAQSV RRVQPVKRTP KTLAKPADSQ
60 70 80 90 100
ESPGEWTTWF NIDHPGGQGD YERLDAIRFY YGERVCARPL RLEARTTDWM
110 120 130 140 150
PAGSTGQVVH GSPREGFWCL NREQRPGQNC SNYTVRFLCP PGSLRGDAEH
160 170 180 190 200
IWSSWSPWSK CSAACGHTGV QTRTRTCLAQ TVSLCSEATE EGQLCMSQAC
210 220 230 240 250
TACDLTCPMG QVNADCDACM CQDFMLHGAI SLPGGGPAPG AAVYLLAKAP
260 270 280 290 300
KMLTRTDSSG RFRVPGLCPD GKTILKITKT KFAPIMITMP KTSLKSATIN
310 320 330 340 350
AEFVRAETPY IVMNPEMKAR RAGQSVSLCC KATGKPSPDK YFWYHNNTLL
360 370 380 390 400
DPSLYKHESK LVLRNLQQDQ AGEYFCKAQS DAGAVKSKVT QLTVIAHDET
410 420 430 440 450
PCNPTPESYL IRLPHDCFQN ASNSFYYDVG RCPIKTCAGQ QDNGIRCRDA
460 470 480 490 500
VENCCGISRT EEREIQCSGY TLPTKVAVEC SCQRCAETRS IVRGRVTATD
510 520 530 540 550
NGEPMRFGHV YMGNNRVSMT GYKGTFTLHI PQDTERLVLT FVDRLQKFVN
560 570 580 590 600
TTKVLPFNKK GSAVFHEIKM LRQKEPITLE AMETNIIPLG EVIGEDPVAE
610 620 630 640 650
LEIPSKSFYR QNGEPFTGKV KASVTFLDPR NISTATAAQS DLNFINDEGD
660 670 680 690 700
TFPLRTYGMF SVDFRDEATS ESLNAGKVKV HLDSTQVKMP EHVPAMKLWS
710 720 730 740 750
LNPDTGLWEE EGDFKFESQR RNKREERTFL VGNMEIRERR LFNLDVPESR
760 770 780 790 800
RCFIKVRTYR SERFLPSEQI QGVVVSVINL EPRTGFSSNP RAWGRFDSVI
810 820 830 840 850
TGPNGACLPA FCDDQSPDAY SVYVLASLSG EELEAVESSP KFNPNAIGVP
860 870 880 890 900
QPYLNKLKYR RTDHEDPRVK KTAFQISMAK PRPNSAEESN GPIYAFENLR
910 920 930 940 950
ACEEAPPSAA HFRFYQIEGD RYDYNTVPFN EDDPMSWTED YLAWWPKPME
960 970 980 990 1000
FRACYIKVKI VGPLEVNVRS RNMGGTHRQT VGKLYGIRDV KSTRDRDQPN
1010 1020 1030 1040 1050
VSSACLEFKC SGMLYDQDRV DRTLVKVIPQ GSCHRASVNS MLHEYLVNHL
1060 1070 1080 1090 1100
PLAVNNDTSE YTMLAPLDPL GHNYGIYTVT DQDPRTAKEI ALGRCFDGTS
1110 1120 1130 1140 1150
DGSSRIMKSN VGVALTFNCA ERQVGRQSAF QYLQSTPARS PATGTVQGRV
1160 1170 1180
PAMRQQRASR GGLRRRGSMA PLRFSGVAQQ PLSN
Length:1,184
Mass (Da):132,334
Last modified:October 11, 2004 - v1
Checksum:iC83B97AC0D0DC9D3
GO

Sequence cautioni

The sequence BAC38252 differs from that shown. Reason: Frameshift at position 1170.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti499T → A in BAC38252 (PubMed:16141072).Curated1
Sequence conflicti499T → A in AAM92572 (Ref. 3) Curated1
Sequence conflicti734M → L in AAM92572 (Ref. 3) Curated1
Sequence conflicti1155Q → E in AAM92572 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081544 mRNA. Translation: BAC38252.1. Frameshift.
BC080666 mRNA. Translation: AAH80666.1.
AY116589 Genomic DNA. Translation: AAM92571.1.
AY116591, AY116590 Genomic DNA. Translation: AAM92572.1.
RefSeqiNP_775561.1. NM_173385.2.
UniGeneiMm.138455.

Genome annotation databases

GeneIDi214425.
KEGGimmu:214425.
UCSCiuc009qcz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081544 mRNA. Translation: BAC38252.1. Frameshift.
BC080666 mRNA. Translation: AAH80666.1.
AY116589 Genomic DNA. Translation: AAM92571.1.
AY116591, AY116590 Genomic DNA. Translation: AAM92572.1.
RefSeqiNP_775561.1. NM_173385.2.
UniGeneiMm.138455.

3D structure databases

ProteinModelPortaliQ66K08.
SMRiQ66K08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036631.

PTM databases

iPTMnetiQ66K08.
PhosphoSitePlusiQ66K08.

Proteomic databases

MaxQBiQ66K08.
PaxDbiQ66K08.
PeptideAtlasiQ66K08.
PRIDEiQ66K08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi214425.
KEGGimmu:214425.
UCSCiuc009qcz.1. mouse.

Organism-specific databases

CTDi8483.
MGIiMGI:2444507. Cilp.

Phylogenomic databases

eggNOGiENOG410IJBD. Eukaryota.
ENOG410XXZR. LUCA.
HOGENOMiHOG000111676.
HOVERGENiHBG081175.
InParanoidiQ66K08.
PhylomeDBiQ66K08.
TreeFamiTF330132.

Miscellaneous databases

PROiQ66K08.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042254.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000884. TSP1_rpt.
IPR025155. WxxW_domain.
[Graphical view]
PfamiPF13330. Mucin2_WxxW. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCILP1_MOUSE
AccessioniPrimary (citable) accession number: Q66K08
Secondary accession number(s): Q7TSS0, Q7TSS1, Q8BV01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.