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Protein

Cartilage intermediate layer protein 1

Gene

Cilp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity).By similarity

GO - Biological processi

  1. negative regulation of insulin-like growth factor receptor signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cartilage intermediate layer protein 1
Short name:
CILP-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cilp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2444507. Cilp.

Subcellular locationi

GO - Cellular componenti

  1. extracellular matrix Source: MGI
  2. extracellular space Source: MGI
  3. extracellular vesicular exosome Source: MGI
  4. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 11841163Cartilage intermediate layer protein 1PRO_0000014674Add
BLAST
Chaini22 – ?724703Cartilage intermediate layer protein 1 C1PRO_0000014675Add
BLAST
Chaini?725 – 1184460Cartilage intermediate layer protein 1 C2PRO_0000014676Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi161 ↔ 195By similarity
Disulfide bondi165 ↔ 200By similarity
Disulfide bondi177 ↔ 185By similarity
Disulfide bondi330 ↔ 376By similarity
Glycosylationi346 – 3461N-linked (GlcNAc...)By similarity
Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi550 – 5501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1000 – 10001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1056 – 10561N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ66K08.
PRIDEiQ66K08.

PTM databases

PhosphoSiteiQ66K08.

Expressioni

Gene expression databases

BgeeiQ66K08.
ExpressionAtlasiQ66K08. baseline and differential.
GenevestigatoriQ66K08.

Interactioni

Subunit structurei

Monomer. Interacts with TGFB1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ66K08.
SMRiQ66K08. Positions 269-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 20152TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini309 – 39385Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiNOG38857.
HOGENOMiHOG000111676.
HOVERGENiHBG081175.
InParanoidiQ66K08.
OrthoDBiEOG780RKM.
PhylomeDBiQ66K08.
TreeFamiTF330132.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR000884. Thrombospondin_1_rpt.
IPR025155. WxxW_domain.
[Graphical view]
PfamiPF13330. Mucin2_WxxW. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66K08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIKTWVFS FLVLEVTTVL GRQTMLAQSV RRVQPVKRTP KTLAKPADSQ
60 70 80 90 100
ESPGEWTTWF NIDHPGGQGD YERLDAIRFY YGERVCARPL RLEARTTDWM
110 120 130 140 150
PAGSTGQVVH GSPREGFWCL NREQRPGQNC SNYTVRFLCP PGSLRGDAEH
160 170 180 190 200
IWSSWSPWSK CSAACGHTGV QTRTRTCLAQ TVSLCSEATE EGQLCMSQAC
210 220 230 240 250
TACDLTCPMG QVNADCDACM CQDFMLHGAI SLPGGGPAPG AAVYLLAKAP
260 270 280 290 300
KMLTRTDSSG RFRVPGLCPD GKTILKITKT KFAPIMITMP KTSLKSATIN
310 320 330 340 350
AEFVRAETPY IVMNPEMKAR RAGQSVSLCC KATGKPSPDK YFWYHNNTLL
360 370 380 390 400
DPSLYKHESK LVLRNLQQDQ AGEYFCKAQS DAGAVKSKVT QLTVIAHDET
410 420 430 440 450
PCNPTPESYL IRLPHDCFQN ASNSFYYDVG RCPIKTCAGQ QDNGIRCRDA
460 470 480 490 500
VENCCGISRT EEREIQCSGY TLPTKVAVEC SCQRCAETRS IVRGRVTATD
510 520 530 540 550
NGEPMRFGHV YMGNNRVSMT GYKGTFTLHI PQDTERLVLT FVDRLQKFVN
560 570 580 590 600
TTKVLPFNKK GSAVFHEIKM LRQKEPITLE AMETNIIPLG EVIGEDPVAE
610 620 630 640 650
LEIPSKSFYR QNGEPFTGKV KASVTFLDPR NISTATAAQS DLNFINDEGD
660 670 680 690 700
TFPLRTYGMF SVDFRDEATS ESLNAGKVKV HLDSTQVKMP EHVPAMKLWS
710 720 730 740 750
LNPDTGLWEE EGDFKFESQR RNKREERTFL VGNMEIRERR LFNLDVPESR
760 770 780 790 800
RCFIKVRTYR SERFLPSEQI QGVVVSVINL EPRTGFSSNP RAWGRFDSVI
810 820 830 840 850
TGPNGACLPA FCDDQSPDAY SVYVLASLSG EELEAVESSP KFNPNAIGVP
860 870 880 890 900
QPYLNKLKYR RTDHEDPRVK KTAFQISMAK PRPNSAEESN GPIYAFENLR
910 920 930 940 950
ACEEAPPSAA HFRFYQIEGD RYDYNTVPFN EDDPMSWTED YLAWWPKPME
960 970 980 990 1000
FRACYIKVKI VGPLEVNVRS RNMGGTHRQT VGKLYGIRDV KSTRDRDQPN
1010 1020 1030 1040 1050
VSSACLEFKC SGMLYDQDRV DRTLVKVIPQ GSCHRASVNS MLHEYLVNHL
1060 1070 1080 1090 1100
PLAVNNDTSE YTMLAPLDPL GHNYGIYTVT DQDPRTAKEI ALGRCFDGTS
1110 1120 1130 1140 1150
DGSSRIMKSN VGVALTFNCA ERQVGRQSAF QYLQSTPARS PATGTVQGRV
1160 1170 1180
PAMRQQRASR GGLRRRGSMA PLRFSGVAQQ PLSN
Length:1,184
Mass (Da):132,334
Last modified:October 11, 2004 - v1
Checksum:iC83B97AC0D0DC9D3
GO

Sequence cautioni

The sequence BAC38252.1 differs from that shown. Reason: Frameshift at position 1170. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti499 – 4991T → A (PubMed:16141072).Curated
Sequence conflicti499 – 4991T → A (Ref. 3) Curated
Sequence conflicti734 – 7341M → L in AAM92572 (Ref. 3) Curated
Sequence conflicti1155 – 11551Q → E in AAM92572 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081544 mRNA. Translation: BAC38252.1. Frameshift.
BC080666 mRNA. Translation: AAH80666.1.
AY116589 Genomic DNA. Translation: AAM92571.1.
AY116591, AY116590 Genomic DNA. Translation: AAM92572.1.
RefSeqiNP_775561.1. NM_173385.2.
UniGeneiMm.138455.

Genome annotation databases

GeneIDi214425.
KEGGimmu:214425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081544 mRNA. Translation: BAC38252.1. Frameshift.
BC080666 mRNA. Translation: AAH80666.1.
AY116589 Genomic DNA. Translation: AAM92571.1.
AY116591, AY116590 Genomic DNA. Translation: AAM92572.1.
RefSeqiNP_775561.1. NM_173385.2.
UniGeneiMm.138455.

3D structure databases

ProteinModelPortaliQ66K08.
SMRiQ66K08. Positions 269-394.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ66K08.

Proteomic databases

PaxDbiQ66K08.
PRIDEiQ66K08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi214425.
KEGGimmu:214425.

Organism-specific databases

CTDi8483.
MGIiMGI:2444507. Cilp.

Phylogenomic databases

eggNOGiNOG38857.
HOGENOMiHOG000111676.
HOVERGENiHBG081175.
InParanoidiQ66K08.
OrthoDBiEOG780RKM.
PhylomeDBiQ66K08.
TreeFamiTF330132.

Miscellaneous databases

NextBioi374308.
PROiQ66K08.
SOURCEiSearch...

Gene expression databases

BgeeiQ66K08.
ExpressionAtlasiQ66K08. baseline and differential.
GenevestigatoriQ66K08.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR000884. Thrombospondin_1_rpt.
IPR025155. WxxW_domain.
[Graphical view]
PfamiPF13330. Mucin2_WxxW. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary tumor.
  3. "The mouse Cilp gene."
    Lorenzo P., Heinegaard D.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51 AND 344-1184.
    Strain: 129/SvJ.

Entry informationi

Entry nameiCILP1_MOUSE
AccessioniPrimary (citable) accession number: Q66K08
Secondary accession number(s): Q7TSS0, Q7TSS1, Q8BV01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: March 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.