Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q66K08 (CILP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cartilage intermediate layer protein 1

Short name=CILP-1
Gene names
Name:Cilp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1184 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage By similarity.

Subunit structure

Monomer. Interacts with TGFB1 By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Post-translational modification

Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion By similarity.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 TSP type-1 domain.

Sequence caution

The sequence BAC38252.1 differs from that shown. Reason: Frameshift at position 1170.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 11841163Cartilage intermediate layer protein 1
PRO_0000014674
Chain22 – ?724703Cartilage intermediate layer protein 1 C1
PRO_0000014675
Chain?725 – 1184460Cartilage intermediate layer protein 1 C2
PRO_0000014676

Regions

Domain150 – 20152TSP type-1
Domain309 – 39385Ig-like C2-type

Amino acid modifications

Glycosylation1291N-linked (GlcNAc...) Potential
Glycosylation1321N-linked (GlcNAc...) Potential
Glycosylation3461N-linked (GlcNAc...) By similarity
Glycosylation4201N-linked (GlcNAc...) Potential
Glycosylation5501N-linked (GlcNAc...) Potential
Glycosylation6311N-linked (GlcNAc...) Potential
Glycosylation10001N-linked (GlcNAc...) Potential
Glycosylation10561N-linked (GlcNAc...) Potential
Disulfide bond161 ↔ 195 By similarity
Disulfide bond165 ↔ 200 By similarity
Disulfide bond177 ↔ 185 By similarity
Disulfide bond330 ↔ 376 By similarity

Experimental info

Sequence conflict4991T → A Ref.1
Sequence conflict4991T → A Ref.3
Sequence conflict7341M → L in AAM92572. Ref.3
Sequence conflict11551Q → E in AAM92572. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q66K08 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: C83B97AC0D0DC9D3

FASTA1,184132,334
        10         20         30         40         50         60 
MAAIKTWVFS FLVLEVTTVL GRQTMLAQSV RRVQPVKRTP KTLAKPADSQ ESPGEWTTWF 

        70         80         90        100        110        120 
NIDHPGGQGD YERLDAIRFY YGERVCARPL RLEARTTDWM PAGSTGQVVH GSPREGFWCL 

       130        140        150        160        170        180 
NREQRPGQNC SNYTVRFLCP PGSLRGDAEH IWSSWSPWSK CSAACGHTGV QTRTRTCLAQ 

       190        200        210        220        230        240 
TVSLCSEATE EGQLCMSQAC TACDLTCPMG QVNADCDACM CQDFMLHGAI SLPGGGPAPG 

       250        260        270        280        290        300 
AAVYLLAKAP KMLTRTDSSG RFRVPGLCPD GKTILKITKT KFAPIMITMP KTSLKSATIN 

       310        320        330        340        350        360 
AEFVRAETPY IVMNPEMKAR RAGQSVSLCC KATGKPSPDK YFWYHNNTLL DPSLYKHESK 

       370        380        390        400        410        420 
LVLRNLQQDQ AGEYFCKAQS DAGAVKSKVT QLTVIAHDET PCNPTPESYL IRLPHDCFQN 

       430        440        450        460        470        480 
ASNSFYYDVG RCPIKTCAGQ QDNGIRCRDA VENCCGISRT EEREIQCSGY TLPTKVAVEC 

       490        500        510        520        530        540 
SCQRCAETRS IVRGRVTATD NGEPMRFGHV YMGNNRVSMT GYKGTFTLHI PQDTERLVLT 

       550        560        570        580        590        600 
FVDRLQKFVN TTKVLPFNKK GSAVFHEIKM LRQKEPITLE AMETNIIPLG EVIGEDPVAE 

       610        620        630        640        650        660 
LEIPSKSFYR QNGEPFTGKV KASVTFLDPR NISTATAAQS DLNFINDEGD TFPLRTYGMF 

       670        680        690        700        710        720 
SVDFRDEATS ESLNAGKVKV HLDSTQVKMP EHVPAMKLWS LNPDTGLWEE EGDFKFESQR 

       730        740        750        760        770        780 
RNKREERTFL VGNMEIRERR LFNLDVPESR RCFIKVRTYR SERFLPSEQI QGVVVSVINL 

       790        800        810        820        830        840 
EPRTGFSSNP RAWGRFDSVI TGPNGACLPA FCDDQSPDAY SVYVLASLSG EELEAVESSP 

       850        860        870        880        890        900 
KFNPNAIGVP QPYLNKLKYR RTDHEDPRVK KTAFQISMAK PRPNSAEESN GPIYAFENLR 

       910        920        930        940        950        960 
ACEEAPPSAA HFRFYQIEGD RYDYNTVPFN EDDPMSWTED YLAWWPKPME FRACYIKVKI 

       970        980        990       1000       1010       1020 
VGPLEVNVRS RNMGGTHRQT VGKLYGIRDV KSTRDRDQPN VSSACLEFKC SGMLYDQDRV 

      1030       1040       1050       1060       1070       1080 
DRTLVKVIPQ GSCHRASVNS MLHEYLVNHL PLAVNNDTSE YTMLAPLDPL GHNYGIYTVT 

      1090       1100       1110       1120       1130       1140 
DQDPRTAKEI ALGRCFDGTS DGSSRIMKSN VGVALTFNCA ERQVGRQSAF QYLQSTPARS 

      1150       1160       1170       1180 
PATGTVQGRV PAMRQQRASR GGLRRRGSMA PLRFSGVAQQ PLSN 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary tumor.
[3]"The mouse Cilp gene."
Lorenzo P., Heinegaard D.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51 AND 344-1184.
Strain: 129/SvJ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK081544 mRNA. Translation: BAC38252.1. Frameshift.
BC080666 mRNA. Translation: AAH80666.1.
AY116589 Genomic DNA. Translation: AAM92571.1.
AY116591, AY116590 Genomic DNA. Translation: AAM92572.1.
RefSeqNP_775561.1. NM_173385.2.
UniGeneMm.138455.

3D structure databases

ProteinModelPortalQ66K08.
SMRQ66K08. Positions 269-394.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ66K08.

Proteomic databases

PaxDbQ66K08.
PRIDEQ66K08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048762; ENSMUSP00000036631; ENSMUSG00000042254.
GeneID214425.
KEGGmmu:214425.

Organism-specific databases

CTD8483.
MGIMGI:2444507. Cilp.

Phylogenomic databases

eggNOGNOG38857.
GeneTreeENSGT00390000008152.
HOGENOMHOG000111676.
HOVERGENHBG081175.
InParanoidQ66K08.
OrthoDBEOG780RKM.
PhylomeDBQ66K08.
TreeFamTF330132.

Gene expression databases

ArrayExpressQ66K08.
BgeeQ66K08.
GenevestigatorQ66K08.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR000884. Thrombospondin_1_rpt.
IPR025155. WxxW_domain.
[Graphical view]
PfamPF13330. Mucin2_WxxW. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMSSF82895. SSF82895. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio374308.
PROQ66K08.
SOURCESearch...

Entry information

Entry nameCILP1_MOUSE
AccessionPrimary (citable) accession number: Q66K08
Secondary accession number(s): Q7TSS0, Q7TSS1, Q8BV01
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot