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Q66JW3

- TOX_MOUSE

UniProt

Q66JW3 - TOX_MOUSE

Protein

Thymocyte selection-associated high mobility group box protein TOX

Gene

Tox

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    May play a role in regulating T-cell development.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi261 – 32969HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymocyte selection-associated high mobility group box protein TOX
    Alternative name(s):
    Thymus high mobility group box protein TOX
    Gene namesi
    Name:Tox
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2181659. Tox.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Thymocyte selection-associated high mobility group box protein TOXPRO_0000244570Add
    BLAST

    Proteomic databases

    PaxDbiQ66JW3.
    PRIDEiQ66JW3.

    PTM databases

    PhosphoSiteiQ66JW3.

    Expressioni

    Tissue specificityi

    Detected in brain, liver, small intestine, spleen, stomach, testis and thymus.1 Publication

    Inductioni

    Up-regulated during the early stages of T-cell differentiation. Down-regulated during later stages of maturation of CD4 and CD8 T-cells. Up-regulated in immature thymocytes upon activation of pre-T-cell receptor or T-cell receptor. Activation of T-cell receptor of mature thymocytes has no effect.1 Publication

    Gene expression databases

    BgeeiQ66JW3.
    GenevestigatoriQ66JW3.

    Structurei

    Secondary structure

    1
    526
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi267 – 2704
    Helixi272 – 28211
    Helixi288 – 29912
    Helixi304 – 33229

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CO9NMR-A251-339[»]
    ProteinModelPortaliQ66JW3.
    SMRiQ66JW3. Positions 257-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ66JW3.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi237 – 25620Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi417 – 4204Poly-Pro

    Sequence similaritiesi

    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG284736.
    GeneTreeiENSGT00560000076898.
    HOGENOMiHOG000230949.
    HOVERGENiHBG051183.
    InParanoidiQ66JW3.
    OMAiCRNPPAQ.
    OrthoDBiEOG7R834J.
    PhylomeDBiQ66JW3.
    TreeFamiTF106481.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q66JW3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDVRFYPPPA QPAAAPAAPC LGPSPCLDPY YCNKFDGENM YMSMTEPSQD    50
    YVPASQSYPG PSLESEDFNI PPITPPSLPD HSLVHLNEVE SGYHSLCHPM 100
    NHNGLLPFHP QTMDLPEITV SNMLGQDGAL LSNSISVMQE IGNAEGAQYS 150
    SHPQMAAMRP RGQPTDIRQQ ASMMQPGQLT TINQSQLSAQ LGLNMGGTNV 200
    AHNSPSPPGS KSATPSPSSS VHEDECEDAS KINGGEKRPA SDMGKKPKTP 250
    KKKKKKDPNE PQKPVSAYAL FFRDTQAAIK GQNPNATFGE VSKIVASMWD 300
    GLGEEQKQVY KKKTEAAKKE YLKQLAAYRA SLVSKSYTDP VDVKTSQPPQ 350
    LVNSKPSVFH GPSQAHSALY LSSHYHQQPG MTPQLTAMHP SLPRNIAPKP 400
    NNQMPVTVSI ANMAVSPPPP LQISPPLHQH LSMQQHQSLA MQQPLGSQLP 450
    MQVQTALHSP TMQQGFTLQP DYQTIINPTS TAAQVVTQAM EYVRSGCRNP 500
    PPQPVDWSTD YCSSGGMQRD KALYLT 526
    Length:526
    Mass (Da):57,203
    Last modified:June 27, 2006 - v2
    Checksum:iF6E7EE5EFCC6CC03
    GO
    Isoform 2 (identifier: Q66JW3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         232-232: I → V
         233-526: Missing.

    Show »
    Length:232
    Mass (Da):24,844
    Checksum:iEBEED93E558EB600
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271L → Q in BAC30091. (PubMed:16141072)Curated
    Sequence conflicti140 – 1401E → D in AAH80732. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei232 – 2321I → V in isoform 2. 1 PublicationVSP_019598
    Alternative sequencei233 – 526294Missing in isoform 2. 1 PublicationVSP_019599Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF472514 mRNA. Translation: AAL78656.1.
    AK029694 mRNA. Translation: BAC26568.1.
    AK038671 mRNA. Translation: BAC30091.1.
    AK051947 mRNA. Translation: BAC34818.1.
    BC080732 mRNA. Translation: AAH80732.1.
    CCDSiCCDS17953.1. [Q66JW3-1]
    RefSeqiNP_663757.3. NM_145711.4. [Q66JW3-1]
    XP_006538007.1. XM_006537944.1. [Q66JW3-1]
    XP_006538008.1. XM_006537945.1. [Q66JW3-1]
    UniGeneiMm.435828.
    Mm.488241.

    Genome annotation databases

    EnsembliENSMUST00000039987; ENSMUSP00000037966; ENSMUSG00000041272. [Q66JW3-1]
    GeneIDi252838.
    KEGGimmu:252838.
    UCSCiuc008rxs.2. mouse. [Q66JW3-1]
    uc008rxt.1. mouse. [Q66JW3-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF472514 mRNA. Translation: AAL78656.1 .
    AK029694 mRNA. Translation: BAC26568.1 .
    AK038671 mRNA. Translation: BAC30091.1 .
    AK051947 mRNA. Translation: BAC34818.1 .
    BC080732 mRNA. Translation: AAH80732.1 .
    CCDSi CCDS17953.1. [Q66JW3-1 ]
    RefSeqi NP_663757.3. NM_145711.4. [Q66JW3-1 ]
    XP_006538007.1. XM_006537944.1. [Q66JW3-1 ]
    XP_006538008.1. XM_006537945.1. [Q66JW3-1 ]
    UniGenei Mm.435828.
    Mm.488241.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CO9 NMR - A 251-339 [» ]
    ProteinModelPortali Q66JW3.
    SMRi Q66JW3. Positions 257-339.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q66JW3.

    Proteomic databases

    PaxDbi Q66JW3.
    PRIDEi Q66JW3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039987 ; ENSMUSP00000037966 ; ENSMUSG00000041272 . [Q66JW3-1 ]
    GeneIDi 252838.
    KEGGi mmu:252838.
    UCSCi uc008rxs.2. mouse. [Q66JW3-1 ]
    uc008rxt.1. mouse. [Q66JW3-2 ]

    Organism-specific databases

    CTDi 9760.
    MGIi MGI:2181659. Tox.

    Phylogenomic databases

    eggNOGi NOG284736.
    GeneTreei ENSGT00560000076898.
    HOGENOMi HOG000230949.
    HOVERGENi HBG051183.
    InParanoidi Q66JW3.
    OMAi CRNPPAQ.
    OrthoDBi EOG7R834J.
    PhylomeDBi Q66JW3.
    TreeFami TF106481.

    Miscellaneous databases

    ChiTaRSi TOX. mouse.
    EvolutionaryTracei Q66JW3.
    NextBioi 387345.
    PROi Q66JW3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q66JW3.
    Genevestigatori Q66JW3.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TOX: an HMG box protein implicated in the regulation of thymocyte selection."
      Wilkinson B., Chen J.-Y., Han P., Rufner K.M., Goularte O.D., Kaye J.
      Nat. Immunol. 3:272-280(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6 X 129S2.
      Tissue: Thymus.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryonic eye, Hypothalamus and Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Embryonic limb.
    4. "Solution structure of the HMG-box domain of thymus high mobility group box protein TOX from mouse."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 251-339.

    Entry informationi

    Entry nameiTOX_MOUSE
    AccessioniPrimary (citable) accession number: Q66JW3
    Secondary accession number(s): Q8BKH9, Q8BYQ5, Q8R4H0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3