Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q66JW3 (TOX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymocyte selection-associated high mobility group box protein TOX
Alternative name(s):
Thymus high mobility group box protein TOX
Gene names
Name:Tox
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in regulating T-cell development. Ref.1

Subcellular location

Nucleus Ref.1.

Tissue specificity

Detected in brain, liver, small intestine, spleen, stomach, testis and thymus. Ref.1

Induction

Up-regulated during the early stages of T-cell differentiation. Down-regulated during later stages of maturation of CD4 and CD8 T-cells. Up-regulated in immature thymocytes upon activation of pre-T-cell receptor or T-cell receptor. Activation of T-cell receptor of mature thymocytes has no effect. Ref.1

Sequence similarities

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q66JW3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q66JW3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     232-232: I → V
     233-526: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Thymocyte selection-associated high mobility group box protein TOX
PRO_0000244570

Regions

DNA binding261 – 32969HMG box
Motif237 – 25620Nuclear localization signal Potential
Compositional bias417 – 4204Poly-Pro

Natural variations

Alternative sequence2321I → V in isoform 2.
VSP_019598
Alternative sequence233 – 526294Missing in isoform 2.
VSP_019599

Experimental info

Sequence conflict271L → Q in BAC30091. Ref.2
Sequence conflict1401E → D in AAH80732. Ref.3

Secondary structure

......... 526
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: F6E7EE5EFCC6CC03

FASTA52657,203
        10         20         30         40         50         60 
MDVRFYPPPA QPAAAPAAPC LGPSPCLDPY YCNKFDGENM YMSMTEPSQD YVPASQSYPG 

        70         80         90        100        110        120 
PSLESEDFNI PPITPPSLPD HSLVHLNEVE SGYHSLCHPM NHNGLLPFHP QTMDLPEITV 

       130        140        150        160        170        180 
SNMLGQDGAL LSNSISVMQE IGNAEGAQYS SHPQMAAMRP RGQPTDIRQQ ASMMQPGQLT 

       190        200        210        220        230        240 
TINQSQLSAQ LGLNMGGTNV AHNSPSPPGS KSATPSPSSS VHEDECEDAS KINGGEKRPA 

       250        260        270        280        290        300 
SDMGKKPKTP KKKKKKDPNE PQKPVSAYAL FFRDTQAAIK GQNPNATFGE VSKIVASMWD 

       310        320        330        340        350        360 
GLGEEQKQVY KKKTEAAKKE YLKQLAAYRA SLVSKSYTDP VDVKTSQPPQ LVNSKPSVFH 

       370        380        390        400        410        420 
GPSQAHSALY LSSHYHQQPG MTPQLTAMHP SLPRNIAPKP NNQMPVTVSI ANMAVSPPPP 

       430        440        450        460        470        480 
LQISPPLHQH LSMQQHQSLA MQQPLGSQLP MQVQTALHSP TMQQGFTLQP DYQTIINPTS 

       490        500        510        520 
TAAQVVTQAM EYVRSGCRNP PPQPVDWSTD YCSSGGMQRD KALYLT 

« Hide

Isoform 2 [UniParc].

Checksum: EBEED93E558EB600
Show »

FASTA23224,844

References

« Hide 'large scale' references
[1]"TOX: an HMG box protein implicated in the regulation of thymocyte selection."
Wilkinson B., Chen J.-Y., Han P., Rufner K.M., Goularte O.D., Kaye J.
Nat. Immunol. 3:272-280(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
Strain: C57BL/6 X 129S2.
Tissue: Thymus.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryonic eye, Hypothalamus and Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryonic limb.
[4]"Solution structure of the HMG-box domain of thymus high mobility group box protein TOX from mouse."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 251-339.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF472514 mRNA. Translation: AAL78656.1.
AK029694 mRNA. Translation: BAC26568.1.
AK038671 mRNA. Translation: BAC30091.1.
AK051947 mRNA. Translation: BAC34818.1.
BC080732 mRNA. Translation: AAH80732.1.
CCDSCCDS17953.1. [Q66JW3-1]
RefSeqNP_663757.3. NM_145711.4. [Q66JW3-1]
XP_006538007.1. XM_006537944.1. [Q66JW3-1]
XP_006538008.1. XM_006537945.1. [Q66JW3-1]
UniGeneMm.435828.
Mm.488241.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CO9NMR-A251-339[»]
ProteinModelPortalQ66JW3.
SMRQ66JW3. Positions 257-339.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ66JW3.

Proteomic databases

PaxDbQ66JW3.
PRIDEQ66JW3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039987; ENSMUSP00000037966; ENSMUSG00000041272. [Q66JW3-1]
GeneID252838.
KEGGmmu:252838.
UCSCuc008rxs.2. mouse. [Q66JW3-1]
uc008rxt.1. mouse. [Q66JW3-2]

Organism-specific databases

CTD9760.
MGIMGI:2181659. Tox.

Phylogenomic databases

eggNOGNOG284736.
GeneTreeENSGT00560000076898.
HOGENOMHOG000230949.
HOVERGENHBG051183.
InParanoidQ66JW3.
OMACRNPPAQ.
OrthoDBEOG7R834J.
PhylomeDBQ66JW3.
TreeFamTF106481.

Gene expression databases

BgeeQ66JW3.
GenevestigatorQ66JW3.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTOX. mouse.
EvolutionaryTraceQ66JW3.
NextBio387345.
PROQ66JW3.
SOURCESearch...

Entry information

Entry nameTOX_MOUSE
AccessionPrimary (citable) accession number: Q66JW3
Secondary accession number(s): Q8BKH9, Q8BYQ5, Q8R4H0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot