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Protein

Thymocyte selection-associated high mobility group box protein TOX

Gene

Tox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in regulating T-cell development.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi261 – 32969HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: GO_Central
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chromatin remodeling Source: GO_Central
  2. regulation of transcription, DNA-templated Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymocyte selection-associated high mobility group box protein TOX
Alternative name(s):
Thymus high mobility group box protein TOX
Gene namesi
Name:Tox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2181659. Tox.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Thymocyte selection-associated high mobility group box protein TOXPRO_0000244570Add
BLAST

Proteomic databases

PaxDbiQ66JW3.
PRIDEiQ66JW3.

PTM databases

PhosphoSiteiQ66JW3.

Expressioni

Tissue specificityi

Detected in brain, liver, small intestine, spleen, stomach, testis and thymus.1 Publication

Inductioni

Up-regulated during the early stages of T-cell differentiation. Down-regulated during later stages of maturation of CD4 and CD8 T-cells. Up-regulated in immature thymocytes upon activation of pre-T-cell receptor or T-cell receptor. Activation of T-cell receptor of mature thymocytes has no effect.1 Publication

Gene expression databases

BgeeiQ66JW3.
GenevestigatoriQ66JW3.

Structurei

Secondary structure

1
526
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi267 – 2704Combined sources
Helixi272 – 28211Combined sources
Helixi288 – 29912Combined sources
Helixi304 – 33229Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CO9NMR-A251-339[»]
ProteinModelPortaliQ66JW3.
SMRiQ66JW3. Positions 257-339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ66JW3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi237 – 25620Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi417 – 4204Poly-Pro

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG284736.
GeneTreeiENSGT00560000076898.
HOGENOMiHOG000230949.
HOVERGENiHBG051183.
InParanoidiQ66JW3.
OMAiCRNPPAQ.
OrthoDBiEOG7R834J.
PhylomeDBiQ66JW3.
TreeFamiTF106481.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q66JW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVRFYPPPA QPAAAPAAPC LGPSPCLDPY YCNKFDGENM YMSMTEPSQD
60 70 80 90 100
YVPASQSYPG PSLESEDFNI PPITPPSLPD HSLVHLNEVE SGYHSLCHPM
110 120 130 140 150
NHNGLLPFHP QTMDLPEITV SNMLGQDGAL LSNSISVMQE IGNAEGAQYS
160 170 180 190 200
SHPQMAAMRP RGQPTDIRQQ ASMMQPGQLT TINQSQLSAQ LGLNMGGTNV
210 220 230 240 250
AHNSPSPPGS KSATPSPSSS VHEDECEDAS KINGGEKRPA SDMGKKPKTP
260 270 280 290 300
KKKKKKDPNE PQKPVSAYAL FFRDTQAAIK GQNPNATFGE VSKIVASMWD
310 320 330 340 350
GLGEEQKQVY KKKTEAAKKE YLKQLAAYRA SLVSKSYTDP VDVKTSQPPQ
360 370 380 390 400
LVNSKPSVFH GPSQAHSALY LSSHYHQQPG MTPQLTAMHP SLPRNIAPKP
410 420 430 440 450
NNQMPVTVSI ANMAVSPPPP LQISPPLHQH LSMQQHQSLA MQQPLGSQLP
460 470 480 490 500
MQVQTALHSP TMQQGFTLQP DYQTIINPTS TAAQVVTQAM EYVRSGCRNP
510 520
PPQPVDWSTD YCSSGGMQRD KALYLT
Length:526
Mass (Da):57,203
Last modified:June 27, 2006 - v2
Checksum:iF6E7EE5EFCC6CC03
GO
Isoform 2 (identifier: Q66JW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     232-232: I → V
     233-526: Missing.

Show »
Length:232
Mass (Da):24,844
Checksum:iEBEED93E558EB600
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271L → Q in BAC30091 (PubMed:16141072).Curated
Sequence conflicti140 – 1401E → D in AAH80732 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei232 – 2321I → V in isoform 2. 1 PublicationVSP_019598
Alternative sequencei233 – 526294Missing in isoform 2. 1 PublicationVSP_019599Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472514 mRNA. Translation: AAL78656.1.
AK029694 mRNA. Translation: BAC26568.1.
AK038671 mRNA. Translation: BAC30091.1.
AK051947 mRNA. Translation: BAC34818.1.
BC080732 mRNA. Translation: AAH80732.1.
CCDSiCCDS17953.1. [Q66JW3-1]
RefSeqiNP_663757.3. NM_145711.4. [Q66JW3-1]
XP_006538007.1. XM_006537944.2. [Q66JW3-1]
XP_006538008.1. XM_006537945.1. [Q66JW3-1]
UniGeneiMm.435828.
Mm.488241.

Genome annotation databases

EnsembliENSMUST00000039987; ENSMUSP00000037966; ENSMUSG00000041272. [Q66JW3-1]
GeneIDi252838.
KEGGimmu:252838.
UCSCiuc008rxs.2. mouse. [Q66JW3-1]
uc008rxt.1. mouse. [Q66JW3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF472514 mRNA. Translation: AAL78656.1.
AK029694 mRNA. Translation: BAC26568.1.
AK038671 mRNA. Translation: BAC30091.1.
AK051947 mRNA. Translation: BAC34818.1.
BC080732 mRNA. Translation: AAH80732.1.
CCDSiCCDS17953.1. [Q66JW3-1]
RefSeqiNP_663757.3. NM_145711.4. [Q66JW3-1]
XP_006538007.1. XM_006537944.2. [Q66JW3-1]
XP_006538008.1. XM_006537945.1. [Q66JW3-1]
UniGeneiMm.435828.
Mm.488241.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CO9NMR-A251-339[»]
ProteinModelPortaliQ66JW3.
SMRiQ66JW3. Positions 257-339.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ66JW3.

Proteomic databases

PaxDbiQ66JW3.
PRIDEiQ66JW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039987; ENSMUSP00000037966; ENSMUSG00000041272. [Q66JW3-1]
GeneIDi252838.
KEGGimmu:252838.
UCSCiuc008rxs.2. mouse. [Q66JW3-1]
uc008rxt.1. mouse. [Q66JW3-2]

Organism-specific databases

CTDi9760.
MGIiMGI:2181659. Tox.

Phylogenomic databases

eggNOGiNOG284736.
GeneTreeiENSGT00560000076898.
HOGENOMiHOG000230949.
HOVERGENiHBG051183.
InParanoidiQ66JW3.
OMAiCRNPPAQ.
OrthoDBiEOG7R834J.
PhylomeDBiQ66JW3.
TreeFamiTF106481.

Miscellaneous databases

ChiTaRSiTox. mouse.
EvolutionaryTraceiQ66JW3.
NextBioi387345.
PROiQ66JW3.
SOURCEiSearch...

Gene expression databases

BgeeiQ66JW3.
GenevestigatoriQ66JW3.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TOX: an HMG box protein implicated in the regulation of thymocyte selection."
    Wilkinson B., Chen J.-Y., Han P., Rufner K.M., Goularte O.D., Kaye J.
    Nat. Immunol. 3:272-280(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6 X 129S2.
    Tissue: Thymus.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic eye, Hypothalamus and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic limb.
  4. "Solution structure of the HMG-box domain of thymus high mobility group box protein TOX from mouse."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 251-339.

Entry informationi

Entry nameiTOX_MOUSE
AccessioniPrimary (citable) accession number: Q66JW3
Secondary accession number(s): Q8BKH9, Q8BYQ5, Q8R4H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: April 1, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.