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Protein

Volume-regulated anion channel subunit LRRC8E

Gene

Lrrc8e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8E
Alternative name(s):
Leucine-rich repeat-containing protein 8E
Gene namesi
Name:Lrrc8e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1919517. Lrrc8e.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity

  • Note: In the absence of LRRC8A, resides primarily in a cytoplasmic compartment, probably the endoplasmic reticulum. Requires LRRC8A for expression at the cell membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Topological domaini44 – 11673ExtracellularSequence analysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence analysisAdd
BLAST
Topological domaini138 – 264127CytoplasmicSequence analysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence analysisAdd
BLAST
Topological domaini286 – 31227ExtracellularSequence analysisAdd
BLAST
Transmembranei313 – 33321HelicalSequence analysisAdd
BLAST
Topological domaini334 – 795462CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Volume-regulated anion channel subunit LRRC8EPRO_0000076251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence analysis
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ66JT1.
PeptideAtlasiQ66JT1.
PRIDEiQ66JT1.

PTM databases

iPTMnetiQ66JT1.
PhosphoSiteiQ66JT1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000046589.
CleanExiMM_LRRC8E.
GenevisibleiQ66JT1. MM.

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8B), possibly to form a heterohexamer. In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052055.

Structurei

3D structure databases

ProteinModelPortaliQ66JT1.
SMRiQ66JT1. Positions 396-788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati535 – 55622LRR 1Add
BLAST
Repeati558 – 57821LRR 2Add
BLAST
Repeati582 – 60322LRR 3Add
BLAST
Repeati605 – 62622LRR 4Add
BLAST
Repeati630 – 65122LRR 5Add
BLAST
Repeati653 – 67422LRR 6Add
BLAST
Repeati676 – 69722LRR 7Add
BLAST
Repeati699 – 72022LRR 8Add
BLAST
Repeati722 – 74423LRR 9Add
BLAST
Repeati745 – 76622LRR 10Add
BLAST

Sequence similaritiesi

Belongs to the LRRC8 family.Curated
Contains 10 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ66JT1.
OMAiFINQLCY.
OrthoDBiEOG091G01ER.
TreeFamiTF331443.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin_like.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 7 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66JT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVAEFKQF TEQQPAFKVL KPWWDVLAEY LTVAMLMIGV FGCTLQVTQD
60 70 80 90 100
KIICLPSHES RENISGAPCQ QLLPQGISEQ MGGLRELSGL KNNLDLQQYS
110 120 130 140 150
FINQLCYETA LHWYAKYFPY LVVIHTLIFM VCTSFWFKFP GTSSKIEHFI
160 170 180 190 200
SILGKCFDSP WTTRALSEVS GENHKGPASG RAMVTTVTTT GAGSGKVGEG
210 220 230 240 250
EKEKVLIEPE KVVSEPPVVT LLDKKEGEQA KALFEKVKKF RVHVEEGDIL
260 270 280 290 300
YSMYIRQTVL KVCKFFAILV YNLIYVEKIS FLVACRVETS EITGYASFCC
310 320 330 340 350
NHTKAHLFSK LAFCYISFVC VYGITCLYTL YWLFHRPLKE YSFRSVREET
360 370 380 390 400
GMNDIPDVKN DFAFMLHLID QYDSLYSKRF AVFLSEVSES RLKQLNLNHE
410 420 430 440 450
WTPEKLRQKL QRNMRGRLEL SLCMLPGLPD TVFELSEVEA LRLEAICDIS
460 470 480 490 500
FPPGLSQLVN LQELSLLHSP ARLPFSSQIF LRDRLKVICV KFEELREVPL
510 520 530 540 550
WVFGLRGLEE LHLEGLFPPE MARGATLESL RELKQLKVLS LRSNAGKVPA
560 570 580 590 600
SVTDVAGHLQ RLSLHNDGAR LLALNSLKKL AVLRELELVA CGLERIPHAI
610 620 630 640 650
FSLGALQELD LKDNHLRSIE EILSFQHCRK LVTLRLWHNQ IAYVPEHVRK
660 670 680 690 700
LRSLEQLYLS HNKLETLPTQ LGQCFGLRLL DLSHNGLRSL PPELGLLQSL
710 720 730 740 750
QHLALSYNAL ESLPDELFFC HKLRTLLLGY NHLTQLSPDV AALQALSRLE
760 770 780 790
LKGNRLETLP EELGDCKGLK KSGLLVEDTL YEGLPAEVRE KMEEE
Length:795
Mass (Da):90,501
Last modified:July 27, 2011 - v2
Checksum:iCC78623A3FC75DB7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331V → M in AAH80783 (PubMed:15489334).Curated
Sequence conflicti731 – 7311N → D in AAH80783 (PubMed:15489334).Curated
Sequence conflicti750 – 7501E → G in AAH80783 (PubMed:15489334).Curated
Sequence conflicti776 – 7761V → A in AAH80783 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123029 Genomic DNA. No translation available.
BC080783 mRNA. Translation: AAH80783.1.
CCDSiCCDS22079.1.
RefSeqiNP_082451.2. NM_028175.2.
XP_006508969.1. XM_006508906.2.
XP_006508970.1. XM_006508907.2.
XP_011240388.1. XM_011242086.1.
UniGeneiMm.25479.

Genome annotation databases

EnsembliENSMUST00000053035; ENSMUSP00000052055; ENSMUSG00000046589.
GeneIDi72267.
KEGGimmu:72267.
UCSCiuc009kth.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123029 Genomic DNA. No translation available.
BC080783 mRNA. Translation: AAH80783.1.
CCDSiCCDS22079.1.
RefSeqiNP_082451.2. NM_028175.2.
XP_006508969.1. XM_006508906.2.
XP_006508970.1. XM_006508907.2.
XP_011240388.1. XM_011242086.1.
UniGeneiMm.25479.

3D structure databases

ProteinModelPortaliQ66JT1.
SMRiQ66JT1. Positions 396-788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052055.

PTM databases

iPTMnetiQ66JT1.
PhosphoSiteiQ66JT1.

Proteomic databases

PaxDbiQ66JT1.
PeptideAtlasiQ66JT1.
PRIDEiQ66JT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053035; ENSMUSP00000052055; ENSMUSG00000046589.
GeneIDi72267.
KEGGimmu:72267.
UCSCiuc009kth.2. mouse.

Organism-specific databases

CTDi80131.
MGIiMGI:1919517. Lrrc8e.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ66JT1.
OMAiFINQLCY.
OrthoDBiEOG091G01ER.
TreeFamiTF331443.

Miscellaneous databases

PROiQ66JT1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046589.
CleanExiMM_LRRC8E.
GenevisibleiQ66JT1. MM.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin_like.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 7 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 8 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRC8E_MOUSE
AccessioniPrimary (citable) accession number: Q66JT1
Secondary accession number(s): E9QNV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.