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Protein

Volume-regulated anion channel subunit LRRC8E

Gene

Lrrc8e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5223345 Miscellaneous transport and binding events

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8E
Alternative name(s):
Leucine-rich repeat-containing protein 8E
Gene namesi
Name:Lrrc8e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1919517 Lrrc8e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 116ExtracellularSequence analysisAdd BLAST73
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 264CytoplasmicSequence analysisAdd BLAST127
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 312ExtracellularSequence analysisAdd BLAST27
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 795CytoplasmicSequence analysisAdd BLAST462

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762511 – 795Volume-regulated anion channel subunit LRRC8EAdd BLAST795

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ66JT1
PaxDbiQ66JT1
PeptideAtlasiQ66JT1
PRIDEiQ66JT1

PTM databases

iPTMnetiQ66JT1
PhosphoSitePlusiQ66JT1

Expressioni

Gene expression databases

BgeeiENSMUSG00000046589
CleanExiMM_LRRC8E
ExpressionAtlasiQ66JT1 baseline and differential
GenevisibleiQ66JT1 MM

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8B), possibly to form a heterohexamer. In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052055

Structurei

3D structure databases

ProteinModelPortaliQ66JT1
SMRiQ66JT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati535 – 556LRR 1Add BLAST22
Repeati558 – 578LRR 2Add BLAST21
Repeati582 – 603LRR 3Add BLAST22
Repeati605 – 626LRR 4Add BLAST22
Repeati630 – 651LRR 5Add BLAST22
Repeati653 – 674LRR 6Add BLAST22
Repeati676 – 697LRR 7Add BLAST22
Repeati699 – 720LRR 8Add BLAST22
Repeati722 – 744LRR 9Add BLAST23
Repeati745 – 766LRR 10Add BLAST22

Sequence similaritiesi

Belongs to the LRRC8 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148937
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ66JT1
KOiK22038
OMAiINQLCYE
OrthoDBiEOG091G01ER
TreeFamiTF331443

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 1 hit
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 8 hits

Sequencei

Sequence statusi: Complete.

Q66JT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVAEFKQF TEQQPAFKVL KPWWDVLAEY LTVAMLMIGV FGCTLQVTQD
60 70 80 90 100
KIICLPSHES RENISGAPCQ QLLPQGISEQ MGGLRELSGL KNNLDLQQYS
110 120 130 140 150
FINQLCYETA LHWYAKYFPY LVVIHTLIFM VCTSFWFKFP GTSSKIEHFI
160 170 180 190 200
SILGKCFDSP WTTRALSEVS GENHKGPASG RAMVTTVTTT GAGSGKVGEG
210 220 230 240 250
EKEKVLIEPE KVVSEPPVVT LLDKKEGEQA KALFEKVKKF RVHVEEGDIL
260 270 280 290 300
YSMYIRQTVL KVCKFFAILV YNLIYVEKIS FLVACRVETS EITGYASFCC
310 320 330 340 350
NHTKAHLFSK LAFCYISFVC VYGITCLYTL YWLFHRPLKE YSFRSVREET
360 370 380 390 400
GMNDIPDVKN DFAFMLHLID QYDSLYSKRF AVFLSEVSES RLKQLNLNHE
410 420 430 440 450
WTPEKLRQKL QRNMRGRLEL SLCMLPGLPD TVFELSEVEA LRLEAICDIS
460 470 480 490 500
FPPGLSQLVN LQELSLLHSP ARLPFSSQIF LRDRLKVICV KFEELREVPL
510 520 530 540 550
WVFGLRGLEE LHLEGLFPPE MARGATLESL RELKQLKVLS LRSNAGKVPA
560 570 580 590 600
SVTDVAGHLQ RLSLHNDGAR LLALNSLKKL AVLRELELVA CGLERIPHAI
610 620 630 640 650
FSLGALQELD LKDNHLRSIE EILSFQHCRK LVTLRLWHNQ IAYVPEHVRK
660 670 680 690 700
LRSLEQLYLS HNKLETLPTQ LGQCFGLRLL DLSHNGLRSL PPELGLLQSL
710 720 730 740 750
QHLALSYNAL ESLPDELFFC HKLRTLLLGY NHLTQLSPDV AALQALSRLE
760 770 780 790
LKGNRLETLP EELGDCKGLK KSGLLVEDTL YEGLPAEVRE KMEEE
Length:795
Mass (Da):90,501
Last modified:July 27, 2011 - v2
Checksum:iCC78623A3FC75DB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33V → M in AAH80783 (PubMed:15489334).Curated1
Sequence conflicti731N → D in AAH80783 (PubMed:15489334).Curated1
Sequence conflicti750E → G in AAH80783 (PubMed:15489334).Curated1
Sequence conflicti776V → A in AAH80783 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123029 Genomic DNA No translation available.
BC080783 mRNA Translation: AAH80783.1
CCDSiCCDS22079.1
RefSeqiNP_082451.2, NM_028175.2
XP_006508969.1, XM_006508906.3
XP_006508970.1, XM_006508907.3
XP_011240388.1, XM_011242086.2
UniGeneiMm.25479

Genome annotation databases

EnsembliENSMUST00000053035; ENSMUSP00000052055; ENSMUSG00000046589
GeneIDi72267
KEGGimmu:72267
UCSCiuc009kth.2 mouse

Similar proteinsi

Entry informationi

Entry nameiLRC8E_MOUSE
AccessioniPrimary (citable) accession number: Q66JT1
Secondary accession number(s): E9QNV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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