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Q66JT0

- WEE2_MOUSE

UniProt

Q66JT0 - WEE2_MOUSE

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Protein

Wee1-like protein kinase 2

Gene

Wee2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.3 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei237 – 2371ATPCurated
Active sitei334 – 3341Proton acceptorPROSITE-ProRule annotation
Metal bindingi339 – 3391Magnesium; via carbonyl oxygenBy similarity
Metal bindingi375 – 3751Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi214 – 2229ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro
  5. protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. female meiotic division Source: UniProtKB
  2. female pronucleus assembly Source: UniProtKB
  3. mitotic nuclear division Source: InterPro
  4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  5. negative regulation of oocyte maturation Source: UniProtKB
  6. peptidyl-tyrosine phosphorylation Source: GOC
  7. regulation of meiosis I Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Short name:
mWee1B
Gene namesi
Name:Wee2
Synonyms:Wee1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:3027899. Wee2.

Subcellular locationi

Cytoplasm. Nucleus
Note: Localizes mainly in the nucleus. Exported from the nucleus to the cytoplasm before germinal vesicle breakdown (GVBD), allowing meiosis resumption.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151S → A: Abolishes phosphorylation and impairs ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage and metaphase II exit during egg activation. 2 Publications
Mutagenesisi15 – 151S → D: Mimicks phosphorylation state, lesading to enhance kinase activity and promote pronuclear formation. 2 Publications
Mutagenesisi167 – 1693Missing: Abolishes nuclear localization. 1 Publication
Mutagenesisi237 – 2371K → M: Loss of function. 2 Publications
Mutagenesisi311 – 3111L → A: Abolishes the cytoplasmic localization; when associated with A-316; A-320 and A-322. 1 Publication
Mutagenesisi316 – 3161L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-320 and A-322. 1 Publication
Mutagenesisi320 – 3201L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-316 and A-322. 1 Publication
Mutagenesisi322 – 3221L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-316 and A-320. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Wee1-like protein kinase 2PRO_0000248080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphoserine; by CaMK2 and PKA2 Publications
Modified residuei71 – 711PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PKA at Ser-15 in vitro, leading to activate kinase activity. Phosphorylation at Ser-15 by CaMK2, leading to increase its activity and promote metaphase II exit during egg activation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ66JT0.

Expressioni

Tissue specificityi

Ovary-specific.1 Publication

Developmental stagei

Detected only in the oocytes at all developmental stages of the follicle including primary, secondary, preantral, and antral follicles with an increase in signal during development. Readily detectable at the mature oocyte, but disappears at E2.5. Detected in germinal vesicle (GV) and metaphase II-stage oocyte (at protein level).2 Publications

Gene expression databases

BgeeiQ66JT0.
CleanExiMM_WEE2.
GenevestigatoriQ66JT0.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038754.

Structurei

3D structure databases

ProteinModelPortaliQ66JT0.
SMRiQ66JT0. Positions 100-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 485278Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili488 – 51427Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1693Nuclear localization signal
Motifi310 – 32415Nuclear export signalAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiQ66JT0.
KOiK06632.
OMAiIHELHIS.
OrthoDBiEOG7N63M9.
PhylomeDBiQ66JT0.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66JT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADTETDQGL NKKLSFSFCE EDTESEGQMT AQDIGGAQSQ KPGREESEEL
60 70 80 90 100
EGPVPPTRDE LHTSLSRDKE SPGPDLWKSV SGPPKCPETP VLHSKSKLPV
110 120 130 140 150
PTNFSTPKNS LGQPEISLLE KLSSRSSRHM RLTPASLMDE KTSLSLVNIN
160 170 180 190 200
PFTPETYRKL LLQSKGKRKT RDYLEETGEE ESKSVQWLPA KRSILQETNM
210 220 230 240 250
ASRYEKEFFE IEKIGVGEFG TVYKCIKRLD GCIYAIKRSA KSFSGLSNEL
260 270 280 290 300
DLHEVYAHAV LGHHPHVVRY YSSWIEDDHV VIQNEYCNGG SLQAAISENT
310 320 330 340 350
ASNNHFQEPK LKDILLQISL GLKYIHNSGM VHLDIKPSNI FICHKMQCDS
360 370 380 390 400
PVGPEEAESE ADWFLNASVM YKIGDLGHAT SISKPKVEEG DTRFLANEIL
410 420 430 440 450
QENYQHLPKA DIFALGLTIA VAAGAESLPI NGDMWHHIRK GNFPEISQEL
460 470 480 490 500
SDDFYGLLKN MIHPAPKERP SAAALARSRI LWPFLEKTDE LQKQLNLEKS
510 520 530 540 550
KTATLKRELK KARHIQTPQR EVHHCYYQIC KGSRHLLVGG RRKAPSSFTR

GTSSV
Length:555
Mass (Da):62,343
Last modified:September 5, 2006 - v2
Checksum:i0D3FA3972C626693
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211E → K in AAH80784. (PubMed:15489334)Curated
Sequence conflicti70 – 701E → G in AAH52883. (PubMed:15489334)Curated
Sequence conflicti98 – 981L → P in AAH52883. (PubMed:15489334)Curated
Sequence conflicti118 – 1181L → R in AAH80784. (PubMed:15489334)Curated
Sequence conflicti138 – 1381M → V in AAH52883. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ011691 mRNA. Translation: AAY44075.1.
AK163344 mRNA. Translation: BAE37310.1.
AK143336 mRNA. Translation: BAE25345.1.
AK139573 mRNA. Translation: BAE24069.1.
BC052883 mRNA. Translation: AAH52883.1.
BC080784 mRNA. Translation: AAH80784.1.
CCDSiCCDS20029.1.
RefSeqiNP_958758.2. NM_201370.2.
XP_006506389.1. XM_006506326.1.
UniGeneiMm.20593.

Genome annotation databases

EnsembliENSMUST00000038907; ENSMUSP00000038754; ENSMUSG00000037159.
GeneIDi381759.
KEGGimmu:381759.
UCSCiuc009bmq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ011691 mRNA. Translation: AAY44075.1 .
AK163344 mRNA. Translation: BAE37310.1 .
AK143336 mRNA. Translation: BAE25345.1 .
AK139573 mRNA. Translation: BAE24069.1 .
BC052883 mRNA. Translation: AAH52883.1 .
BC080784 mRNA. Translation: AAH80784.1 .
CCDSi CCDS20029.1.
RefSeqi NP_958758.2. NM_201370.2.
XP_006506389.1. XM_006506326.1.
UniGenei Mm.20593.

3D structure databases

ProteinModelPortali Q66JT0.
SMRi Q66JT0. Positions 100-510.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000038754.

Proteomic databases

PRIDEi Q66JT0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038907 ; ENSMUSP00000038754 ; ENSMUSG00000037159 .
GeneIDi 381759.
KEGGi mmu:381759.
UCSCi uc009bmq.2. mouse.

Organism-specific databases

CTDi 494551.
MGIi MGI:3027899. Wee2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063230.
HOGENOMi HOG000004824.
HOVERGENi HBG005050.
InParanoidi Q66JT0.
KOi K06632.
OMAi IHELHIS.
OrthoDBi EOG7N63M9.
PhylomeDBi Q66JT0.
TreeFami TF101088.

Miscellaneous databases

NextBioi 402507.
PROi Q66JT0.
SOURCEi Search...

Gene expression databases

Bgeei Q66JT0.
CleanExi MM_WEE2.
Genevestigatori Q66JT0.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Wee1B is an oocyte-specific kinase involved in the control of meiotic arrest in the mouse."
    Han S.J., Chen R., Paronetto M.P., Conti M.
    Curr. Biol. 15:1670-1676(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION AT SER-15, MUTAGENESIS OF SER-15.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg and Ovary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg.
  4. "Identification and characterization of human Wee1B, a new member of the Wee1 family of Cdk-inhibitory kinases."
    Nakanishi M., Ando H., Watanabe N., Kitamura K., Ito K., Okayama H., Miyamoto T., Agui T., Sasaki M.
    Genes Cells 5:839-847(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumption."
    Oh J.S., Han S.J., Conti M.
    J. Cell Biol. 188:199-207(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 167-LYS--LYS-169; LYS-237; LEU-311; LEU-316; LEU-320 AND LEU-322.
  6. "Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes."
    Oh J.S., Susor A., Conti M.
    Science 332:462-465(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, PHOSPHORYLATION AT SER-15, MUTAGENESIS OF SER-15 AND LYS-237.

Entry informationi

Entry nameiWEE2_MOUSE
AccessioniPrimary (citable) accession number: Q66JT0
Secondary accession number(s): Q4U4S4, Q7TPV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: November 26, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3