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Protein

Eukaryotic translation initiation factor 3 subunit J-B

Gene

Eif3j2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.IEP:

GO - Molecular functioni

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit J-BIEP:
Short name:
eIF3j-BIEP:
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 1-BIEP:
eIF-3-alpha-BIEP:
eIF3 p35IEP:
Gene namesi
Name:Eif3j2
Synonyms:Eif3s1-2, Gm9781
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:3704486. Eif3j2.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedND:
ChainiPRO_00001235072 – 263Eukaryotic translation initiation factor 3 subunit J-BAdd BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineND:1
Modified residuei16PhosphoserineIEP:HMP:1
Modified residuei18PhosphoserineIEP:HMP:1
Modified residuei25PhosphoserineIEP:HMP:1
Cross-linki111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)HMP:
Modified residuei114PhosphothreonineIEP:HMP:1
Modified residuei132PhosphoserineIEP:HMP:1
Modified residuei259PhosphotyrosineHMP:1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.IEP:

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ66JS6.
PaxDbiQ66JS6.
PeptideAtlasiQ66JS6.
PRIDEiQ66JS6.

PTM databases

iPTMnetiQ66JS6.
PhosphoSitePlusiQ66JS6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000043424.
GenevisibleiQ66JS6. MM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.IEP:

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054421.

Structurei

3D structure databases

ProteinModelPortaliQ66JS6.
SMRiQ66JS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 74Sufficient for interaction with EIF3BIEP:Add BLAST69
Regioni248 – 263Promotes stable association with the 40S ribosomeIEP:Add BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili75 – 140IEP:Add BLAST66

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.IEP:

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4813. Eukaryota.
ENOG4111U5G. LUCA.
GeneTreeiENSGT00390000018400.
HOGENOMiHOG000238746.
HOVERGENiHBG066230.
InParanoidiQ66JS6.
KOiK03245.
OMAiNTICGID.
OrthoDBiEOG091G141O.
PhylomeDBiQ66JS6.
TreeFamiTF101514.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j. 1 hit.
InterProiView protein in InterPro
IPR023194. eIF3-like_dom_sf.
IPR013906. eIF3j.
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiView protein in Pfam
PF08597. eIF3_subunit. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66JS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAAAAA AAAGDSDSWD ADTFSMEDPV RKVAGGGTAG GDRWEGEDED
60 70 80 90 100
EDVKDNWDDD DDENKEEAEV KPEVKISEKK KIAEKIKEKE RQQKKRQEEI
110 120 130 140 150
KKRLEEPEES KVLTPEEQLA DKLRLKKLQE ESDLELAKET FGVNNTVYGI
160 170 180 190 200
DAMNPSSRDD FTEFGKLLKD KITQYEKSLY YASFLEALVR DVCISLEIDD
210 220 230 240 250
LKKITNSLTV LCSEKQKQEK QSKAKKKKKG VVPGGGLKAT MKDDLADYGG
260
YEGGYVQDYE DFM
Length:263
Mass (Da):29,486
Last modified:October 11, 2004 - v1
Checksum:i7040416E40650960
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191D → N in BAC38399 (PubMed:16141072).IKR:1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC080788 mRNA. Translation: AAH80788.1.
AK082048 mRNA. Translation: BAC38399.1.
CCDSiCCDS57122.1.
RefSeqiNP_001242984.1. NM_001256055.1.
UniGeneiMm.329403.
Mm.458184.

Genome annotation databases

EnsembliENSMUST00000057110; ENSMUSP00000054421; ENSMUSG00000043424.
GeneIDi100042807.
KEGGimmu:100042807.
UCSCiuc008mab.3. mouse.

Similar proteinsi

Entry informationi

Entry nameiEI3JB_MOUSE
AccessioniPrimary (citable) accession number: Q66JS6
Secondary accession number(s): Q8BUW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: November 22, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families