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Q66JQ7 (CASC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CASC5
Alternative name(s):
Cancer susceptibility candidate gene 5 protein homolog
Kinetochore-null protein 1
Gene names
Name:Casc5
Synonyms:Knl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1612 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore By similarity.

Subunit structure

Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromerekinetochore By similarity. Note: Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle By similarity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16121612Protein CASC5
PRO_0000235983

Regions

Repeat723 – 8271051
Repeat923 – 10271052
Region723 – 10273052 X 104 AA approximate repeats
Motif1577 – 159014Nuclear localization signal Potential

Amino acid modifications

Modified residue321Phosphoserine By similarity
Modified residue601Phosphoserine By similarity
Modified residue7941Phosphoserine By similarity

Experimental info

Sequence conflict1281D → G in AAH80815. Ref.2
Sequence conflict3751H → R in AAH80815. Ref.2
Sequence conflict11511P → L in AAH80815. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q66JQ7 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: A9BE2BB20E008621

FASTA1,612179,337
        10         20         30         40         50         60 
MDGVYSEANE ENDNTQRPVR RQHSSILKPP RSPLQDLKCG NQTNQEPNPP RKRKSSRRVS 

        70         80         90        100        110        120 
FADTIKVFQT ESHMKTERNS EISGMNTLLC APIQTQMQQK EFSITDCNHE RKHANDQTVI 

       130        140        150        160        170        180 
FSDENQMDLT ASHTVMITKG LSDCTKNENS TKIDTTSFLE NLKHHAANSR IKKDLACSTV 

       190        200        210        220        230        240 
SLSQNIFSEK INSDNFIKRL KTGKHISSST ELDKENAEIP VYSKDSNSAS STYQMHASLG 

       250        260        270        280        290        300 
VDENSSNRTR IFREQDDGMN LTQCHTACIK TWIPPSTEAK IGEFKGDKTI YGNECMELTT 

       310        320        330        340        350        360 
NYTIQVLSSE NNLSERETQT QNGMNVTTVD GATAPAPEKK TALKDKLNAA FQGSFPNPEN 

       370        380        390        400        410        420 
KIHIIKCHPI ESETHTVTQI SSQSASTLAV TSKSICSSPA IEGYKTIFHS SSNDAMELTK 

       430        440        450        460        470        480 
CLSAMEEEKK LLKADDKYSK ICTNPDAGPL REKTIYLEED SMDITKSHTV AIDNKIFKHD 

       490        500        510        520        530        540 
QENIKKEIIA IPIFEKEMVL RNLMPMSKDE KRDVNYISVP QVSKESLQRS QTNTLSVSLT 

       550        560        570        580        590        600 
DKKMEFLADE DMDLTKSHTT KLSQVIPTTF DLASKNVTKS YSHSKSPLNE WESLDKQVVL 

       610        620        630        640        650        660 
GQHSKLPLPQ RKDRDDPDCS HHKIMYSEEL QTMDLTKSHT IVIGFGPSEV QEHSKINLEH 

       670        680        690        700        710        720 
KNSQLTAESI QTAVNVPAAN SRVVTTNDMD MLKDRSTHKP ELLKEKQNIK IYGRKSIGRL 

       730        740        750        760        770        780 
KIDKTILFSE GNEGDMDITK SCTVKINHRS LLDKHDSHLV SLAGTSKTIL HARGQVEMEI 

       790        800        810        820        830        840 
NRSHTTPLEC KIISPSDITP GDLDKTMMSI DDHEELDMTK SHTVFIDYQA EAKGVLPDRL 

       850        860        870        880        890        900 
DFQLSKKESL QKPKVTSLAE EIYISKNSES NHLPAKGSQL TILEEGSNSG LGEETNDAQK 

       910        920        930        940        950        960 
PGFLNELLSG KTQRRKSLSL KNKSITFPEN DKSYREIPQS SAVEINNETR LEDRKGFSFV 

       970        980        990       1000       1010       1020 
PLAGTSKPVL SAYGPEDMEI SIGQTTASEY KTVPPEEITT IPMDKTVMFV DNFGDLDVTR 

      1030       1040       1050       1060       1070       1080 
SHTVFIDCQA KEKVLDEYTN LGIQKTKTLS GSEGDTHIQE ITKNPAAQHK HHMTTVIPSS 

      1090       1100       1110       1120       1130       1140 
TVVSDQSSMK IKFHKADRDE EVKGKEVEAN MLKQTKPESC LLNITDGKNV DFTSSYTADV 

      1150       1160       1170       1180       1190       1200 
CRSSDKYSSL PNISSSDNSG GNTMSLCDKN KEKAYNCQVP NEFTYAAILP STYHMDSKKL 

      1210       1220       1230       1240       1250       1260 
SVFPPCPSKE VTQTESAIAL LKDEDPVEEP LGEMATFNSK HVSLNLAKDQ TEAFVDVSVA 

      1270       1280       1290       1300       1310       1320 
SQPHLSAQQS PSTQKGQDVA RRDEGILAKA GKKALPFLLE NVAASTWENE SKIPTNVEHF 

      1330       1340       1350       1360       1370       1380 
AVTYEKELSI SIQTDKCNTN VQSPSNSALT TQVIQTHANA EGALDFLVPS TVSCFSSTKP 

      1390       1400       1410       1420       1430       1440 
SLSNLNRKTE EVLDFQTVNL LPPAEQLLEE GSQAHSMSIV QATEIYRLGS RNDRDEESKT 

      1450       1460       1470       1480       1490       1500 
FCNEAETTSV PLKTAVKDKT RRCSLGIFLP KLPSKRSCSI TGVDDLEQIL ADAADLTQLE 

      1510       1520       1530       1540       1550       1560 
TQPVCSKDPG IGSVAAKLNL SPSQFINEEN LPVYPGEILS SDSVSLDIEE SVLIDTSQRE 

      1570       1580       1590       1600       1610 
SLPSENKTEN CRAQKRTRVE ENDVTNEKKI RTHDSAQDQE VSCLEPKNVL EF 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryonic germ cell.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-507.
Strain: C57BL/6J.
Tissue: Testis and Tongue.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL772264 Genomic DNA. Translation: CAM45955.1.
BC080815 mRNA. Translation: AAH80815.1.
AK009772 mRNA. Translation: BAB26494.1.
AK033132 mRNA. Translation: BAC28167.2.
UniGeneMm.202518.
Mm.392877.

3D structure databases

ProteinModelPortalQ66JQ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ66JQ7. 10 interactions.
MINTMINT-8174094.

PTM databases

PhosphoSiteQ66JQ7.

Proteomic databases

PaxDbQ66JQ7.
PRIDEQ66JQ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028799; ENSMUSP00000028799; ENSMUSG00000027326.
UCSCuc008ltb.1. mouse.

Organism-specific databases

MGIMGI:1923714. Casc5.

Phylogenomic databases

eggNOGNOG116910.
GeneTreeENSGT00410000025918.
HOGENOMHOG000246954.
HOVERGENHBG080986.
OrthoDBEOG7DNNV8.

Gene expression databases

ArrayExpressQ66JQ7.
BgeeQ66JQ7.
CleanExMM_CASC5.
GenevestigatorQ66JQ7.

Family and domain databases

ProtoNetSearch...

Other

PROQ66JQ7.
SOURCESearch...

Entry information

Entry nameCASC5_MOUSE
AccessionPrimary (citable) accession number: Q66JQ7
Secondary accession number(s): A3KGI4, Q8CCH6, Q9CV38
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot